The mechanism by which parasitoid wasp Asobara japaonoca

寄生蜂 Asobara japaonoca 的机制

• 批准号: 24658053
• 负责人: HAYAKAWA Yoichi
• 金额: $ 2.66万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658053/
• 关键词: 寄生蜂; 毒液; 細胞性免疫; 体液性免疫; セリンプロテアーゼ; 宿主昆虫; ショウジョウバエ; 殺虫成分; 側輸卵管成分; 解毒成分; 自然免疫; 細胞生免疫

Endoparasitoid wasp Asobara japonica utilizes Drosophila melanogaster and the other broadly related Drosophila species as habitual hosts by using a notable feature of parasitism strategy. A. japonica females possess highly toxic venom and they inject the venom components with a lethal dose for the hosts at parasitism. A few seconds after the venom injection, they introduce eggs together with the lateral oviduct components that neutralize the detrimental effects of the venom toxin. In the present study, We demonstrated that the venom components impaired the host cellular immunity but did not affect the humoral immunity. Furthermore, injection of the venom components activated a serine protease-like enzyme activity approximately 100 times in the plasma 4 h after the injection. The neutralizing factor in the oviduct blocked the protease-like enzyme activation, while it was unable to neutralize the venom-induced detriment of the host hemocytes.

内寄生蜂Asobara japonica利用黑腹果蝇（Drosophila melanogaster）和其他广泛相关的果蝇属物种作为习惯性宿主，其寄生策略具有显著特征。A.日本雌虫具有剧毒的毒液，在寄生时注入对寄主有致死剂量的毒液成分。在注射毒液后几秒钟，它们将卵与侧输卵管成分一起引入，以中和毒液毒素的有害影响。本研究表明，蛇毒成分对宿主的细胞免疫功能有一定的损害作用，但对体液免疫功能无明显影响。此外，注射的毒液成分激活了丝氨酸蛋白酶样酶的活性约100倍，在血浆中注射后4小时。输卵管中的中和因子阻断了蛋白酶样酶的激活，但不能中和毒液对宿主血细胞的损害。

项目成果

Stimulation of Vitellogenin gene expression by permethrin in the brown planthopper, Nilaparvata lugens (Hemiptera : Auchenorrhyncha : Delphacidae)

氯菊酯对褐飞虱 (Nilaparvata lugens) 中卵黄蛋白原基因表达的刺激（半翅目：Aucheno rhyncha：飞虱科）

• 发表时间: 2014
• 期刊: J. Pesticide Sci.
• 作者: Khaeso,K.;Matsumoto;H.;Hayakawa;Y.;and Tojo;S.
• 通讯作者: S.

寄生婆知asobara japonica毒液殺虫成分が宿主ショウジョウバエに及ぼす生理機能

寄生竹螈毒液杀虫成分对宿主果蝇的生理功能

• 发表时间: 2013
• 作者: 降幡駿介;早川洋一
• 通讯作者: 早川洋一

Biological functions of insect cytokine growth-blocking peptide (GBP)

昆虫细胞因子生长阻断肽（GBP）的生物学功能

• 发表时间: 2012
• 作者: 降幡駿介;早川洋一;Yoichi Hayakawa
• 通讯作者: Yoichi Hayakawa

Gain of long tonic immobility behavioral trait causes the red flour beetle to reduce anti-stress capacity

• DOI: 10.1016/j.jinsphys.2013.11.008
• 发表时间: 2014-01-01
• 期刊: JOURNAL OF INSECT PHYSIOLOGY
• 影响因子: 2.2
• 作者: Kiyotake, Hikaru;Matsumoto, Hitoshi;Hayakawa, Yoichi
• 通讯作者: Hayakawa, Yoichi

Characteristics common to a cytokine family spanning five orders of insects

• DOI: 10.1016/j.ibmb.2012.03.001
• 发表时间: 2012-06-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Matsumoto, Hitoshi;Tsuzuki, Seiji;Hayakawa, Yoichi
• 通讯作者: Hayakawa, Yoichi