Studies on free radical scavengers of microbial origin

微生物来源的自由基清除剂的研究

• 批准号: 02660129
• 负责人: HAYAKAWA Yoichi
• 金额: $ 1.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660129/
• 关键词: Free radicals; Lipid peroxidation; Streptomyces; Benthocyanin; Phenazine; benthocyanin

Oxygen-derived free radicals have been known to play a role in a variety of diseases such as inflammation, atherosclerosis, cerebral ischemia and cardiovascular diseases. In the course of a screening for free radical scavengers of microbial origin, an actinomycete identified as Streptomyces prunicolor was found to produce three new active substances, designated benthocyanins A, B and C. The structures of the benthocyanins were elucidated to consist of a modified phenazine chromophore with a phenyl group and a geranyl side chain based on NNM and mass spectral analysis.Benthocyanins A, B and C inhibited lipid peroxidation in rat liver microsomes at the IC_<50>s of 0.29, 0.16 and 0.26 mug/ml, respectively. The benthocyanins also inhibited hemolysis of rat erythrocytes by a radical producer, AAPH. The IC_<50>s were 0.42 mug/ml for benthocyanin A, 0.92 mug/ml for B and 0.64 mug/ml for C.

氧自由基在炎症、动脉粥样硬化、脑缺血和心血管疾病等多种疾病中发挥重要作用。在筛选微生物来源的自由基清除剂的过程中，发现一种被鉴定为Prunicolor链霉菌的放线菌产生三种新的活性物质，命名为底花青素A、B和C。根据NNM和质谱分析，确定了底花青苷的结构为含苯基和香叶基侧链的吩嗪修饰发色团，底花青苷A、B和C抑制大鼠肝微粒体脂质过氧化作用的IC_<50>s分别为0.29、0.16和0.26 μ g/ml。底花青素还能抑制自由基产生者AAPH对大鼠红细胞的溶血作用。对<50>底花青A、B和C的IC_ s分别为0.42 μ g/ml、0.92 μ g/ml和0.64 μ g/ml。

项目成果

Kazuo Shin-ya: "Benthocyanins B and C,new free radical scavengers from Streptomyces prunicolor." Journal of Organic Chemistry.

Kazuo Shin-ya：“Benthocyanins B 和 C，来自 Streptomyces prunicolor 的新型自由基清除剂。”

Kazuo Shin-ya: "The structure of benthocyanin A,a new free radical scavenger of microbial origin" Tetrahedron Letters. 32. 943-946 (1991)

Kazuo Shin-ya：“微生物来源的新型自由基清除剂底色青素 A 的结构”四面体快报。

Kazuo Shin-ya: "The structure of benthocyanin A, a new free radical scavenger of microbial origin." Tetrahedron Letters. 32. 943-946 (1991)

Kazuo Shin-ya：“微生物来源的新型自由基清除剂 Benthocyanin A 的结构。”

Kazuo Shin-ya: "Benthocyanins B and C, new free radical scavengers from Streptomyces prunicolor." Journal of Organic Chemistry.

Kazuo Shin-ya：“Benthocyanins B 和 C，来自 Streptomyces prunicolor 的新型自由基清除剂。”

Kazuo Shinーya: "The structure of benthocyanin A,a new free radical scavenger of microbial" Tetrahedron Letters. 32. 943-946 (1991)

Kazuo Shin-ya：“微生物自由基清除剂 Benthoyanin A 的结构”Tetrahedron Letters 32. 943-946 (1991)。