Nucletide sequencing of DNA topoisomerase II gene for diagnosis of fungal infection

DNA 拓扑异构酶 II 基因的核苷酸测序用于诊断真菌感染

• 批准号: 11670262
• 负责人: KANBE Toshio
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670262/
• 关键词: Candida; Aspergillus; Cryptococcus; DNA topoisomerase II; molecular diagnosus; identification; PCR; phylogenetic relationship; 塩基配列; プライマー; 混合感染; 病原性真菌; クリプトコッカサス; トポイソメラーゼII; 診断

We have determined the nucleotide sequences of about 55 % region of DNA topoisomerase II gene (approximately 2.3 kb) isolated from the pathogenic Candida species, C.dubliniensis, C.parapsilosis, C.tropicalis, C.krusei, C.kefyr, C.guilliermondii and C.lusitaniae. Evolutionary relationships among nine Candida species including those of C.albicans and C.glabrata were studied based on the DNA topoisomerase II gene. The nucleotide sequences of 2192 bp, that covered two catalytic domains ; ATPase and cutting/resealing, were subjected for phylogenetic analysis. Sequence comparison and evolutionary analysis have revealed that the Candida species tested here are not monophyletic, and the two strains within the species C.tropicalis and C.parapsilosis are too diverse to be in a single species. Wide variety of the divergence was observed among the functional domains of DNA topoisomerase II, suggesting that Candida species were in the different evolutionary paths at least as regarding DNA topoisomerase II gene. Sequence information and the observation on species-specific manner of molecular evolution of DNA topoisomerase II in Candida will be applied for developing a method of identification and characterization of the Candida species in both natural and clinical isolates.

我们测定了从致病念珠菌、杜氏假丝酵母菌、近缘假丝酵母菌、热带假丝酵母菌、克鲁斯假丝酵母菌、克氏假丝酵母菌、克氏假丝酵母菌、假丝酵母菌和卢氏假丝酵母菌分离的DNA拓扑异构酶II基因约55%的核苷酸序列(约2.3kb)。基于DNA拓扑异构酶II基因，对白念珠菌和光滑念珠菌等9种念珠菌的进化关系进行了研究。2192bp的核苷酸序列覆盖了ATPase和切割/再密封两个催化区，用于系统发育分析。序列比较和进化分析表明，这里测试的念珠菌不是单系种，热带假丝酵母菌和近缘假丝酵母菌的两个菌株太过多样化，不可能属于一个物种。DNA拓扑异构酶II的功能域之间存在着很大的差异，这表明念珠菌至少在DNA拓扑异构酶II基因上处于不同的进化路径。通过对念珠菌DNA拓扑异构酶II的序列信息和物种特异性分子进化方式的观察，将建立一种鉴定和鉴定自然分离株和临床分离株中念珠菌种类的方法。

项目成果

Yuko Yoshikawa et al: "Formation of a giant troid from long duplex DNA"Langmuir. 15. 4085-4088 (1999)

Yuko Yoshikawa 等人：“从长双链 DNA 形成巨型 troid”Langmuir。

Yuko Yoshikawa, Shin-ichirou M.Nimura, Toshio Kanbe and Kenichi Yoshikawa: "Controlling the folding/unfolding transition of the DNA-histone H1 complex by direct optical manipulation."Chem.Phys.Lett.. 330. 77-82 (2000)

Yuko Yoshikawa、Shin-ichirou M.Nimura、Toshio Kanbe 和 Kenichi Yoshikawa：“通过直接光学操作控制 DNA-组蛋白 H1 复合物的折叠/展开转变。”Chem.Phys.Lett.. 330. 77-82 (2000

Yukiko Matsuzawa et al.: "Visualization and optical trapping of an individual submicrometer-sized assembly in aqueous solution : Aminated polyethylene glycol (PEG-A) complexed with palmitic acid and DNA in plovethyleneglycol (PEG) solution"J.AM.,Che.,Soc.

Yukiko Matsuzawa 等人：“水溶液中单个亚微米尺寸组装体的可视化和光学捕获：胺化聚乙二醇 (PEG-A) 与聚乙二醇 (PEG) 溶液中的棕榈酸和 DNA 复合”J.AM.，Che。

Yukiko Matsuzawa et al.: "Visualization and optical trapping of an individual submicrometer-sized assembly in aqueous solution : Aminated polyethylene glycol (PEG-A) complexed with palmitic acid and DNA in ployethyleneglycol (PEG) solution"J.Am.,Che.,Soc.

Yukiko Matsuzawa 等人：“水溶液中单个亚微米尺寸组装体的可视化和光学捕获：氨基化聚乙二醇 (PEG-A) 与棕榈酸和 DNA 在聚乙二醇 (PEG) 溶液中复合”J.Am.，Che。

S.-I.Iwaguchi, T.Kanbe, T.Tohne, P.T.Magee, and T.Suzuki: "High-frequency occurrence of chromosome translocation in a mutant strain of Candida albicans by a suppressor mutation of ploidy shift."Yeast. 16. 411-422 (2000)

S.-I.Iwaguchi、T.Kanbe、T.Tohne、P.T.Magee 和 T.Suzuki：“通过倍性转移的抑制突变，在白色念珠菌突变株中高频发生染色体易位。”酵母。