The influence of anesthetics on myocardial tissue ultrastructural integrity after ischemic preconditioning.

麻醉剂对缺血预处理后心肌组织超微结构完整性的影响。

• 批准号: 11671502
• 负责人: KITAHATA Hiroshi
• 金额: $ 2.3万
• 依托单位: Tokushima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671502/
• 关键词: Ischemic preconditioning; Stunned myocardium; Myocardial tissue characterization; Integrate backscatter; Cardiac function; Volatile anesthetics; Sevoflurane

Twenty-five mongrel dogs were divided into three groups : fentanyl group (n=8), sevoflurane group (n=9), and sevoflurane-IP group (n=8). Anesthesia was maintained with continuous infusion of fentanyl (0.3 μg/kg/min) in fentanyl group, and with 1 MAC sevoflurane in sevoflurane and sevoflurane-IP groups. The volume conductance catheter and the pressure-transducer-tipped catheter were placed in the left ventricle. Integrated backscatter (IBS) images of left ventricular short-axis view at the mid-papillary muscle level were obtained from the heart surface using a 12-MHz ultrasonic transducer. Regions of interest were designated in the midseptum, supplied by the left descending coronary artery (LAD), and the inferior wall supplied by the right coronary artery. The magnitudes of cyclic variation of IBS and % systolic wall thickening (%WT) in each region were determined. The ratio of stroke work to pressure volume area (SW/PVA) was used to evaluate the left ventricular work efficiency. Both f … More entanyl and sevoflurane groups underwent 20 min of LAD occlusion, followed by 120 min of reperfusion. The sevoflurane-IP group was pretreated with two 5-min LAD occlusions interspersed with 15-min periods of reperfusion. Hemodynamic and echocardiographic variables were recorded simultaneously before LAD occlusion (baseline), 20 min after occlusion (immediately before reperfusion), 20, 60, and 120 min after reperfusion.In all groups, %WT in the LAD area decreased significantly following LAD occlusion, however, %WT 20 min after occlusion in the IP group was significantly higher than those in fentanyl and sevoflurane groups (13±8%, -2±13%, -7±13%, respectively). In fentanyl and sevoflurane groups, IBS cyclic variations in the LAD area and SW/PVA significantly decreased immediately before and, 20, 60, and 120 min after reperfusion. In sevoflurane-IP group, IBS cyclic variations did not decrease significantly at the same time intervals, and SW/PVA was maintained.Ischemic preconditioning under sevoflurane anesthesia preserved regional systolic function, myocardial tissue ultrastructural integrity, and left ventriculo-arterial coupling, but did not sevoflurane alone. Less

25只杂种犬随机分为芬太尼组（n=8）、七氟醚组（n=9）和七氟醚IP组（n=8）。芬太尼组以芬太尼0.3 μg/kg/min持续输注维持麻醉，七氟醚组和七氟醚IP组以1 MAC七氟醚维持麻醉。将容量传导导管和压力传感器头端导管置于左心室。使用12 MHz超声换能器从心脏表面获得中乳头肌水平左心室短轴视图的背向散射积分（IBS）图像。感兴趣区域被指定在由左冠状动脉降支（LAD）供血的中间隔和由右冠状动脉供血的下壁中。测定每个区域IBS和收缩期室壁增厚%（%WT）的周期性变化幅度。以每搏作功与压力容积面积比值（SW/PVA）评价左室作功效率。两个f ...更多信息 芬太尼组和七氟醚组分别行LAD阻断20 min，再灌注120 min。七氟醚IP组预处理两个5分钟的LAD闭塞，间隔15分钟的再灌注期。封堵前同步记录血流动力学和超声心动图参数（基线），闭塞后20分钟在所有组中，LAD区域的%WT在LAD阻断后显著降低，然而，IP组阻断后20 min的%WT显著高于芬太尼组和七氟醚组（分别为13± 8%，-2 ± 13%，-7 ± 13%）。芬太尼组和七氟醚组在再灌注前即刻、再灌注后20、60和120 min，LAD区IBS周期性变化和SW/PVA均显著降低。七氟醚-IP组在相同的时间间隔内IBS周期变化没有显著降低，SW/PVA保持不变。七氟醚麻醉下的缺血预处理保留了局部收缩功能、心肌组织超微结构完整性和左室-动脉耦联，但单独使用七氟醚则没有。少

项目成果

Kazuhiro Mori, et al.: "Cyclic variation of integrated ultrasound backscatter in the left ventricle during the early neonatal period."American Heart Journal. 140. 463-468 (2000)

Kazuhiro Mori 等人：“新生儿早期左心室综合超声反向散射的循环变化。”美国心脏杂志。

Kazuhiro Mori, et al.: "Cyclic variation of integrated ultrasound backscatter in the left ventricle during the early neonatal period."American Heart Journal. 140・(3). 463-468 (2000)

Kazuhiro Mori 等人：“新生儿早期左心室中的综合超声反向散射的循环变化。”美国心脏杂志 140・(3) (2000)。

Hiroshi Kitahata: "Myocardial perfusion with contrast material : Transesophageal Echocardiography, Part III, Chapter 21"Edited by Ryozo Omoto and Yasu Oka, Shindan-to-Chiryosya, Tokyo. 360 (2000)

Hiroshi Kitahata：“造影剂心肌灌注：经食管超声心动图，第三部分，第 21 章”由 Ryozo Omoto 和 Yasu Oka 编辑，Shindan-to-Chiryosya，东京。

Hiroshi kitahata: "Recent advances in transesophageal echocardiography:Myocardial perfusion.Chapter 8.Part 3"Shindan-to-Chiryosya,Tokyo,edited by Ryozo Omoto and Yasu Oka (in press). (2000)

Hiroshi kitahata：“经食管超声心动图的最新进展：心肌灌注。第 8 章第 3 部分”Shindan-to-Chiryosya，东京，由 Ryozo Omoto 和 Yasu Oka 编辑（出版中）。