The influence of anesthetics on myocardial tissue ultrastructural integrity after ischemic preconditioning.

麻醉剂对缺血预处理后心肌组织超微结构完整性的影响。

• 批准号: 11671502
• 负责人: KITAHATA Hiroshi
• 金额: $ 2.3万
• 依托单位: Tokushima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671502/
• 关键词: Ischemic preconditioning; Stunned myocardium; Myocardial tissue characterization; Integrate backscatter; Cardiac function; Volatile anesthetics; Sevoflurane

Twenty-five mongrel dogs were divided into three groups : fentanyl group (n=8), sevoflurane group (n=9), and sevoflurane-IP group (n=8). Anesthesia was maintained with continuous infusion of fentanyl (0.3 μg/kg/min) in fentanyl group, and with 1 MAC sevoflurane in sevoflurane and sevoflurane-IP groups. The volume conductance catheter and the pressure-transducer-tipped catheter were placed in the left ventricle. Integrated backscatter (IBS) images of left ventricular short-axis view at the mid-papillary muscle level were obtained from the heart surface using a 12-MHz ultrasonic transducer. Regions of interest were designated in the midseptum, supplied by the left descending coronary artery (LAD), and the inferior wall supplied by the right coronary artery. The magnitudes of cyclic variation of IBS and % systolic wall thickening (%WT) in each region were determined. The ratio of stroke work to pressure volume area (SW/PVA) was used to evaluate the left ventricular work efficiency. Both f … More entanyl and sevoflurane groups underwent 20 min of LAD occlusion, followed by 120 min of reperfusion. The sevoflurane-IP group was pretreated with two 5-min LAD occlusions interspersed with 15-min periods of reperfusion. Hemodynamic and echocardiographic variables were recorded simultaneously before LAD occlusion (baseline), 20 min after occlusion (immediately before reperfusion), 20, 60, and 120 min after reperfusion.In all groups, %WT in the LAD area decreased significantly following LAD occlusion, however, %WT 20 min after occlusion in the IP group was significantly higher than those in fentanyl and sevoflurane groups (13±8%, -2±13%, -7±13%, respectively). In fentanyl and sevoflurane groups, IBS cyclic variations in the LAD area and SW/PVA significantly decreased immediately before and, 20, 60, and 120 min after reperfusion. In sevoflurane-IP group, IBS cyclic variations did not decrease significantly at the same time intervals, and SW/PVA was maintained.Ischemic preconditioning under sevoflurane anesthesia preserved regional systolic function, myocardial tissue ultrastructural integrity, and left ventriculo-arterial coupling, but did not sevoflurane alone. Less

将25只杂种狗分为三组：芬太尼组（n=8）、七氟烷组（n=9）和七氟烷-IP组（n=8）。芬太尼组持续输注芬太尼（0.3 μg/kg/min），七氟醚组和七氟醚-IP组持续输注1 MAC七氟醚维持麻醉。将容量电导导管和带有压力传感器尖端的导管放置在左心室中。使用 12 MHz 超声换能器从心脏表面获得中乳头肌水平左心室短轴视图的集成反向散射 (IBS) 图像。感兴趣的区域被指定在由左冠状动脉降支（LAD）供应的中隔，以及由右冠状动脉供应的下壁。确定每个区域的 IBS 周期性变化幅度和收缩期壁增厚百分比 (%WT)。每搏做功与压力容积面积之比（SW/PVA）用于评价左心室做功效率。恩太尼组和七氟醚组均接受 20 分钟的 LAD 闭塞，然后进行 120 分钟的再灌注。七氟烷-IP 组接受两次 5 分钟的 LAD 闭塞术，中间进行 15 分钟的再灌注。在 LAD 封堵前（基线）、封堵后 20 分钟（再灌注前）、再灌注后 20、60 和 120 分钟同时记录血流动力学和超声心动图变量。 在所有组中，LAD 封堵后 LAD 区域的 %WT 显着下降，然而，IP 组封堵后 20 分钟的 %WT 显着高于芬太尼和七氟醚组 （分别为13±8%、-2±13%、-7±13%）。在芬太尼和七氟醚组中，LAD 区域和 SW/PVA 的 IBS 周期性变化在再灌注前以及再灌注后 20、60 和 120 分钟显着降低。在七氟烷-IP组中，IBS周期变化在相同的时间间隔内没有显着减少，并且SW/PVA得以维持。七氟烷麻醉下的缺血预处理保留了局部收缩功能、心肌组织超微结构完整性和左心室-动脉耦合，但单独七氟烷则没有。较少的

项目成果

Kazuhiro Mori, et al.: "Cyclic variation of integrated ultrasound backscatter in the left ventricle during the early neonatal period."American Heart Journal. 140. 463-468 (2000)

Kazuhiro Mori 等人：“新生儿早期左心室综合超声反向散射的循环变化。”美国心脏杂志。

Hiroshi kitahata: "Recent advances in transesophageal echocardiography:Myocardial perfusion.Chapter 8.Part 3"Shindan-to-Chiryosya,Tokyo,edited by Ryozo Omoto and Yasu Oka (in press). (2000)

Hiroshi kitahata：“经食管超声心动图的最新进展：心肌灌注。第 8 章第 3 部分”Shindan-to-Chiryosya，东京，由 Ryozo Omoto 和 Yasu Oka 编辑（出版中）。

Kazuhiro Mori, et al.: "Cyclic variation of integrated ultrasound backscatter in the left ventricle during the early neonatal period."American Heart Journal. 140・(3). 463-468 (2000)

Kazuhiro Mori 等人：“新生儿早期左心室中的综合超声反向散射的循环变化。”美国心脏杂志 140・(3) (2000)。

Hiroshi Kitahata: "Myocardial perfusion with contrast material : Transesophageal Echocardiography, Part III, Chapter 21"Edited by Ryozo Omoto and Yasu Oka, Shindan-to-Chiryosya, Tokyo. 360 (2000)

Hiroshi Kitahata：“造影剂心肌灌注：经食管超声心动图，第三部分，第 21 章”由 Ryozo Omoto 和 Yasu Oka 编辑，Shindan-to-Chiryosya，东京。