Effects of ageing on localizations of immunocompetent cells in periapical lesions in the rat mandibular molar

衰老对大鼠下颌磨牙根尖周病变中免疫活性细胞定位的影响

• 批准号: 11671914
• 负责人: YAMAZAKI Yasushi
• 金额: $ 2.18万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671914/
• 关键词: periapical lesions; ageing; immunocompetent cells; 免疫組織学的染色; 根尖病巣; 免疫組織学的深色; 加齢

Periapical lesions have been shown to be induced mainly by local immune defense reactions against bacterial infection and inflammatory reactions. It has also been shown that the immune system and tissue repair are impaired in older animals : especially, T-cell dependent, cell mediated immunity reacts less efficiently with increasing age. Thus, ageing may be one of factors influencing the process of periapical lesions. Our aims here were to compare localizations of immunocompetent cells after experimental periapical lesions in molar teeth between young and old rats, and to elucidate the effects of ageing on the processes of periapical lesions.Eighteen old (16 month-old) and eighteen young (2 month-old) male Wistar rats were used. To induce periapical lesions, a pulpectomy was performed on the mandlbular first molar. Then, their own excrement dissolved in a sterile broth medium was infused into the root canal. At 4, 7, 14 and 28 days after pulp treatment, the animals were anaesthetized w … More ith pentobarbital and perfused with 4% parafolmaldehyde solution. In each animal, the mandibles were dissected free, immersed in the same fixative solution for a day, and then decalcified in 14% EDTA solution at 4℃ for 2 months. The mandibles were embedded in paraffin wax. Mesio-distal, longitudinal sections were cut serially at a thickness of 5 μm. Immunoperoxidase staining was done on sections by the use of monoclonal antibodies OX-6 (anti-class II molecules) , ED1 (a pan-macrophage antibody, reactive also with dendritic cells), and ED2 (anti-tissue macrophages).The present observation indicated that the processes of experimental periapical lesions were different between young and old rats. In the old rats, the inflammatory reactions around the periapical lesions appeared to be less severe, and to become chronic, and the repair tended to delay, as compared with the young rats. (1) localization of ED1-positive cells : In 16 month-old rats, there appeared to be many ED1-positive mononuclear and polynuclear cells in periapical lesions and surrounding tissues at 4 and 7 days ; at 14 and 28 days, ED1-positive polynuclear cells were still seen adjacent to bone surfaces from apical to apex. In 2 month-old rats, at 28 days ED1-positive cells appeared to decrease. (2) localizations of ED2 and 0X6-positive cells : In 16 month-old rats, distributions and numbers of ED2 and 0X6-positive cells appeared to be similar from 4 to 14 days, but the positive cells tended to increase at 28 days in the periodontal ligament at apical and mid-root levels. In 2 month-old rats, there appeared to be no differences in localizations of those positive cells between 7 and 28 days. We assume that the chronic inflammatory reactions in the old rats may be due to the impairment with ageing of the immune mechanisms where macrophages, lymphocytes, and plasma cells play main roles. Less

根尖周病变主要是由针对细菌感染和炎症反应的局部免疫防御反应引起的。研究还表明，免疫系统和组织修复在老年动物中受损：特别是，T细胞依赖性细胞介导的免疫反应随着年龄的增长而降低。因此，年龄可能是影响根尖周病变过程的因素之一。本实验采用18只老年（16月龄）和18只青年（2月龄）雄性Wistar大鼠，比较老年和青年大鼠实验性根尖周病变后免疫活性细胞的分布，并探讨年龄对根尖周病变过程的影响。为了诱导根尖周病变，在下颌第一磨牙上进行牙髓切除术。然后，将他们自己的排泄物溶解在无菌肉汤培养基中注入根管。在牙髓处理后4、7、14和28天，动物被麻醉， ...更多信息 用戊巴比妥钠灌胃，4%对甲醛溶液灌胃。在每只动物中，将下颌骨游离解剖，浸入相同的固定液中1天，然后在4℃下在14%EDTA溶液中脱钙2个月。下颌骨被埋在石蜡中。以5 μm的厚度连续切割近远中纵向切片。用单克隆抗体OX-6（抗Ⅱ类分子）、ED 1（泛巨噬细胞抗体，也与树突状细胞反应）和ED 2（抗组织巨噬细胞）对切片进行免疫过氧化物酶染色，结果表明，青年和老年大鼠根尖周病变的过程不同。与年轻大鼠相比，老年大鼠根尖周病变周围的炎症反应似乎不那么严重，并且变得慢性，修复倾向于延迟。(1)ED 1阳性细胞的定位：在16个月大的大鼠中，在第4天和第7天，在根尖周病变和周围组织中似乎有许多ED 1阳性单核细胞和多核细胞;在第14天和第28天，仍然可以看到ED 1阳性多核细胞从根尖到根尖邻近骨表面。在2个月大的大鼠中，在28天时，ED 1阳性细胞似乎减少。(2)ED 2和OX 6阳性细胞的定位：在16个月大的大鼠中，ED 2和OX 6阳性细胞的分布和数量从4到14天似乎是相似的，但是在根尖和中根水平的牙周膜中，阳性细胞在28天趋于增加。在2个月大的大鼠中，这些阳性细胞在7天和28天之间的定位似乎没有差异。我们推测，老年大鼠的慢性炎症反应可能是由于免疫机制的损伤，其中巨噬细胞，淋巴细胞和浆细胞发挥主要作用。少

项目成果

土田眞美, 滝澤 久, 他: "根尖部病変の発現における老化の影響"日本歯科保存学雑誌. 43. 1074-1082 (2000)

Mami Tsuchida、Hisashi Takizawa 等人：“衰老对根尖周病变发展的影响”日本保守牙科杂志 43. 1074-1082 (2000)。

Mami Tsuchida, Hisashi Takizawa, Yukiko Kaneda, Yasushi Yamazaki, Atsuko Seno, Takayuki Koshino, Shu Tanaka, Kenzo Kawasaki, Jiro Nakamura, Takashi Aral: "The Effect of Aging on Experimental Periapical Lesions"The Japanese Journal of Conservative Dentistr

Mami Tsuchida、Hisashi Takizawa、Yukiko Kaneda、Yasushi Yamazaki、Atsuko Seno、Takayuki Koshino、Shu Tanaka、Kenzo Kawasaki、Jiro Nakamura、Takashi Aral：“老化对实验性根尖周病变的影响”日本保守牙科杂志

Y.Yamazaki, K.Komatsu, et al.: "The effects of a high-cerbohydrate diet on the stress-strain behavior of the periodontal ligament of the distal root of the mandibular first molar in hamsters"J Periodontal Res.. 36. 301-308 (2001)

Y.Yamazaki、K.Komatsu 等人：“高碳水化合物饮食对仓鼠下颌第一磨牙远端根部牙周韧带应力应变行为的影响”J 牙周研究 36。

Y. Yamazaki, K. Komatsu, T. Arai, M. Chiba: "The effects of a high-carbohydrate diet on the stress-strain behavior of the periodontal ligament of the distal root of the mandibular first molar in hamsters"J Periodontal Res. Vol. 36. 301-308 (2001)

Y. Yamazaki、K. Komatsu、T. Arai、M. Chiba：“高碳水化合物饮食对仓鼠下颌第一磨牙远端根部牙周韧带应力应变行为的影响”J 牙周研究