Long term stability of titanimum with hydroxyapatite layer precipitated by anodic oxidation and hydrothermal treatment

通过高压蒸汽水热处理(SA处理)，在阳极氧化的钛表面沉积了结晶度高、与钛基材结合力强的羟基磷灰石涂层，改善了化学纯钛的表面性能。报道了SA治疗的有效骨传导能力。SA处理后的羟基磷灰石层在口腔环境下的稳定性在临床上具有重要意义。因此，它检查了SA处理层的长期稳定性。采用纯钛圆盘。在含有0.01mol/Lβ-甘油磷酸二钠五水溶液和0.15mol/L一水醋酸钙的溶液中进行阳极氧化。施加350V的电解电压，阳极氧化后，用蒸馏水清洗并烘干。水热处理在300℃下进行2小时。将这些样品溶解到0.9%的氯化钠溶液中，以获得…，以备pH=6.0时更多的醋酸和Tris-HCl。用扫描电子显微镜和X射线衍射仪对样品进行了分析。第1天和第7天的扫描电子显微镜观察结果显示，溶解后的HA结构没有发生变化。14天后，HA晶体内可见溶解，但未承认HA晶体全部溶解的图像。经X射线衍射分析，不允许因溶解天数和溶液的不同而发生变化。从大鼠骨髓细胞中获得的成骨细胞和破骨细胞在样品表面进行培养。培养物用卡尔诺夫斯基的S法和四氧化三铯固定，用分级系列乙醇脱水，用叔丁醇冷冻干燥。扫描电子显微镜下观察标本。在培养过程中，细胞贴附于所有标本表面并产生大量骨结节。浸渍样品和未浸渍样品的密度和形成速度没有差别。在形态上，所有样本的破骨细胞都没有差异。提示SA处理的羟基磷灰石涂层对体外培养细胞具有长期稳定性。较少

The surface property of chemically pure titanium was improved depositing a hydroxyapatite layer which exhibited high crystallinity and strong adhesion to the titanium substrate on an anodically oxidized titanium surface through the hydrothermal treatment with high pressure steam (SA treatment). The effective bone conduction ability of SA treatment was reported. The stability of SA treated hydroxyapatite layer under oral environment is important in the clinical case. Therefore, it examined a long term stability of the SA treated layer. Pure titanium discs were employed. They were anodized in a solution containing 0.01mol/l β-glycerophosphate disodium salt pentahydrate and 0.15mol/l calcium acetate monohydrate. An electrolytic voltage of 350V was applied, and after anodic oxidation the discs were washed with distilled water and dried. Hydrothermal treatment was the conducted at 300 ℃ for 2 hours. These samples were dissolved to the 0.9%NaCl solution to have prepared for pH=6.0 by gaining … More acetic acid and Tris-HCl. The samples were analyzed by SEM and X-rays diffraction analysis. The result of the observation in the SEM of the 1st and 7th day's samples, the change of the HA structure was not admitted after dissolution. After 14 days, dissolution was seen within the HA crystals, but the image that all the HA crystals are dissolving, was not admitted. As a result of X-rays diffraction analysis, the change by the difference of the dissolution number of days and solution were not admitted.Osteoblasts and osteoclasts that obtained from rat bone marrow cells, were cultured on the surface of samples. Cultures were fixed with Karnovsky' s method and osmium tetroxide, dehydrated through a graded series of ethanol, and freeze dried with t-butyl alcohol. Specimens were observed under SEM.During the culture period, the cells adhered to all samples surfaces and produced an abundant amount of bone nodules. The density and speed of formation were not different between dipping samples and non-dipping samples. Morphologically, there was no difference concerning osteoclasts for all samples. It was suggested that hydroxyapatite layer of SA treatment has long term stability for culture cells in vitro. Less