Study on Escherichia coli O-157-induced renal damage

脂多糖（LPS）和维罗毒素-2 （VT2）参与了大肠杆菌o -157诱导的肾损害。然而，在注射每种药物后，病理生理特征并没有得到充分的评估。进行这项研究就是为了解决这个问题。分别于9:00和21:00向Wistar大鼠静脉注射LPS （5 mg/kg）对大鼠slps的时间毒性，并观察其器官损伤情况。21:00组器官损伤程度明显大于9:00组。在21:00的试验中，血清白细胞介素-6 （IL-6，一种炎症细胞因子）的升高更大，其在器官中的合成也更增强。这些数据表明，LPS的毒性取决于其给药时间，可能是通过IL-6反应的时间依赖性差异。抗中性粒细胞抗体对vt_2诱导的小鼠器官损伤的预防作用。分别于VT_2后0和24 h腹腔注射VT_2 (100 ng)，同时和不同时注射抗中性粒细胞小鼠抗体。单独注射VT_2后，血浆中性粒细胞显著升高，所有动物均在注射VT_2后5 d内死亡。抗中性粒细胞抗体小鼠的死亡率显著降低。这些数据表明中性粒细胞在vt_2诱导的器官损伤中起一定作用。

Lipopolysaccharide (LPS) and verotoxin-2 (VT2) are involved in the Escherichia coli O-157-induced renal damage. However, pathophysiologic profiles are not fully evaluated after an injection of each agent. This study was undertaken to address this issue.1. Chronotoxicity of LPS in ratsLPS (5 mg/kg) was injected intravenously to Wistar rats at 9:00 or 21:00, and organ damages were evaluated. The degree of organ damages in the 21:00 trial were significantly greater than those in the 9:00 trial. The elevation in serum interleukin-6 (IL-6), an inflammatory cytokine, was greater and its synthesis in organs was more enhanced in the 21:00 trial. These data indicate that the toxicity of LPS depends on its dosing time, probably through the time-dependent difference in the IL-6 response.2. Preventive effect of anti-neutrophil antibody against the VT_2-induced organ damage in mice.VT_2 (100 ng) was injected intraperitoneally to C57BL/6 mice with and without the co-administration of anti-neutrophil mouse antibody at 0 and 24 hours following VT_2. After the injection of VT_2 alone, plasma neutrophil remarkably elevated and all animals died within 5 days after the VT_2 injection. Death rate was significantly reduced in mice with the anti-neutrophil antibody. These data suggest that neutrophil plays some role in the VT_2-induced organ damage.