Development of A Next Generation Injector by Near IR Video-Bioanalysis

通过近红外视频生物分析开发下一代注射器

• 批准号: 11794033
• 负责人: MASUJIMA Tsutomu
• 金额: $ 4.61万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794033/
• 关键词: near IR video-scope; Injector; Injector Needle; Medical Devices; Injector for Next Generation; 近赤外; 画像解析

1. Analyses of the needle injection into the skin by near IRvideo-scopeCurrent shape of the injection needles was investigated and the process of injection of the needle into the skin was analyzed by image analysis using near IR video-scope. It was found that the needle top of the injector cut off the skin in flat way and then the tube of the needle go into this space which will make pain.2. Analysis of silicon oil attachment on to the skin by observation of video-mass scope.Video-mass scope was developed and it was applied to investigate the attachment of silicon oil onto the skin where the needle was injected. The silicon oil which was coated on the needle of the injector, was transferred onto the injection point of the skin and stayed there even after the taking out.3. The analysis of injection wound recovery using a time lapse video-scope methodThe process of the recovery of injection wound was investigated using model cell layer of confluent 3T3 cells. Time lapse video scope showed that the recovery was accelerated by the presence of inside components of mast cell which was destroyed by the injection of the needle through the skin layer.4. Laser processing of the needle top using Nd : YAG laserA Nd : YAG laser was found to be used to make the surface of the needle top to be smooth in order to decrease the pain on insertion of the needle and also used to make the hole in the top.5. Development of the next generation injector and patent authorizationBased on these results, some types of the next generation injectors were proposed and be applied for patents. 1) the processing of the top sharp which will make less-painful injection, 2) the smoothing of the internal face of the needle top to make less pain, 3) oval shape needle to make less pain, 4) the needle with pain scattering ring or adaptor, 5) small needle with separated blood vacuum correction tube, and these idea were applied for patents.

1.近红外显微镜对皮肤注射过程的分析利用近红外显微镜对注射针的电流形态进行了研究，并对注射针的注射过程进行了图像分析。发现注射器针尖与皮肤平切，针管进入该间隙，产生疼痛.应用电视包块镜观察硅油在皮肤上的附着情况，研制了电视包块镜，并将其应用于硅油在注射部位皮肤上的附着情况的观察。将涂在注射器针头上的硅油转移到皮肤的注射点上，即使在取出后仍留在那里。应用时间推移电视显微镜观察注射创面愈合过程用3T3细胞融合模型细胞层观察注射创面愈合过程。时间推移视频显示，通过皮肤层注射针头破坏肥大细胞的内部成分的存在加速了恢复.使用Nd：YAG激光器对针头进行激光处理发现Nd：YAG激光器可用于使针头表面光滑，以减少针头插入时的疼痛，也可用于在针头上打孔。下一代喷油器的开发与专利授权在此基础上，提出了下一代喷油器的几种类型，并申请了专利。1)针尖的加工使注射疼痛减轻，2）针尖内表面的光滑使注射疼痛减轻，3）椭圆形针使注射疼痛减轻，4）带有疼痛分散环或适配器的针，5）带有分离式血液真空校正管的小针，这些构思已申请专利。

项目成果

T.Masujima, N.Ojima: "Scientific Experiments --Guarding life from invader--"Maruzen T.Tuda Ed.. 160 (2001)

T.Masujima、N.Ojima：“科学实验——保护生命免受入侵者——”Maruzen T.Tuda Ed.. 160 (2001)

S.Inagaki, T.Shingaki, T.Masujima et. al: "Analysis of molecular talk between cells by video-microscope"Proceeding of PSJ 121 congress. 121(4). 149 (2000)

S.Inagaki、T.Shingaki、T.Masujima 等。

N.Ojima,T.Shingaki,T.Masujima et.al.: "Droplet Electro-Coupling Between Capillary Electrophoresis and Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectroscopy and Its Application"Electrophoresis. 22(6)(In Press). (2001)

N.Ojima、T.Shingaki、T.Masujima 等人：“毛细管电泳与基质辅助激光解吸电离飞行时间质谱之间的液滴电耦合及其应用”电泳。

稲垣祥子, 新垣知輝, 尾島典行, 升島 努, 他: "ビデオ-マイクロスコープ法による細胞間分子トーク解析"日本薬学会第121年会要旨集. 121(4). 149 (2000)

Shoko Inagaki、Tomoteru Aragaki、Noriyuki Ojima、Tsutomu Masushima 等：“使用视频显微镜法分析细胞间分子对话”日本药学会第 121 届年会记录 121(4)。

Y.Sugawara, T.Masujima et.al: "Odor distinctiveness between enantiomers of linalool : Difference in perception and responses elicited by sensory test and forehead surface potentical wave measurement"Chem.Senses. 25. 77-84 (2000)

Y.Sukawara、T.Masujima 等人：“芳樟醇对映体之间的气味独特性：感官测试和前额表面电位波测量引起的感知和反应差异”Chem.Senses。