Development of Capillary Electrophoresis under Crossed Super Magnetic Field

交叉超磁场下毛细管电泳的研究进展

• 批准号: 06452377
• 负责人: MASUJIMA Tsutomu
• 金额: $ 4.03万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06452377/
• 关键词: Magnetic Field; Capillary Electrophoresis; Separation; Video Analysis; ビデオ分析

1) Development of Capillary Electrophoresis under Crossed Super Magnetic FieldA coiled capillary was inserted into the gap of a super-strong permanent magnet. When the electric field was applied to this capillary, it was found that the current showed less value than that without magnetic field. The positional isomers, i.e.p-aminosalicylic acid, p-aminobenzoic acid, 1,8-antheaquinon disulfonic acid, which were hard to resolve, were separated under the magnet with much higher resolution. Similar effect can be obtained even with lowering of temperature of capillary.2) Video Monitoring of Separating Process for Capillary ElectrophoresisSeparating process was successful monitored by our developed chromato-videoscope. The band shows rise up for the leading side of band but shows tailing for the rear side according to the migration distance. With evidences of many aspects of separating process, new applications and improvements were innovated.3) New Methods for Capillary Electrophoresis(1) De … More termination of Dissolved Oxygen in Water Samples Using Capillary ElectrophoresisDissolved oxygen in water samples was analyzed by CE for the first time. Each analysis was performed within 5 min and as low as 50ppb dissolved oxygen can be determined by this method and this method was successfully applied to the analysis of river water.(2) Improvement in the Determination of Food Additive Dye stuffs by Capillary Electrophoresis using B-Cyclodextrin. It was found that the inclusion of B-cyclodextrin into solvent for separation improves the reproducibility and peak shape. Taking this advantage, seven food dye stuffs were successfully separated. It was shown that this method is simple and rapid.(3) Development of Non-aqueous Capillary Electrophoresis (NACE) SystemIn contrast to the usual aqueous phase CE,we charanged to develop the method of non-aqueous CE.The NACE has many advantages in the reproducibility and applicability to various kinds of organic solutes. We discussed the future potentials of these newly developed CE methods in each publication. Less

1)交叉超强磁场下毛细管电泳法的发展将螺旋毛细管插入超强永磁体的缝隙中。当电场作用于该毛细管时，其电流表现出比不加磁场时更小的值。在磁铁的作用下，对氨基水杨酸、对氨基苯甲酸、1，8-苯喹酮二磺酸等难于拆分的位置异构体得到了较高的分离。2)毛细管电泳色谱分离过程的视频监控我们研制的色谱仪成功地对分离过程进行了监控。根据迁徙距离的不同，乐队表演在乐队的前部上升，但在后部表现为尾部。3)毛细管电泳法新方法(1)De…毛细管电泳法测定水样中的溶解氧首次用毛细管电泳法测定了水样中的溶解氧。每次分析在5分钟内完成，最低可测定50 ppb的溶解氧，并成功应用于河水的分析。(2)改进了B-环糊精毛细管电泳法测定食品添加剂染料的方法。研究发现，将B-环糊精包合到溶剂中进行分离，提高了分离的重现性和峰形。利用这一优势，成功分离了7种食用染料。非水相毛细管电泳法(NACE)的建立与常规的水相毛细管电泳法相比，我们发展了非水相毛细管电泳法，NACE在重现性和对各种有机溶质的适用性方面具有许多优点。我们在每一篇文章中都讨论了这些新开发的CE方法的未来潜力。较少

项目成果

S.Razee: "The Effect of a Crossed Magnetic Field on Capillary Electrophoresis" Chem.Pharm.Bull.42. 2376-2378 (1994)

S.Razee：“交叉磁场对毛细管电泳的影响”Chem.Pharm.Bull.42。

S.Razee, A.Tamura, T.Masujima: ""The effect of a Crossed Magnet Field on Capillary Electrophoresis"" Chem.Pharm.Bull.42. 2376-2378 (1994)

S.Razee、A.Tamura、T.Masujima：“交叉磁场对毛细管电泳的影响”Chem.Pharm.Bull.42。

S.Emara ; S.Razee, A.N.El-Shorbagi, T.Masujima: "Flow Injection Method for the Determination of Methotrexate with a Column-Packed Oxidizing Agent" Analyst. 121 (in press). (1996)

S.埃马拉；

A.Tamura, T.Masujima: ""Development of Chromato-Videoscopy-the concepts and foundamental applications" (Japanese)" Jasco Report. 37. 1-6 (1995)

A.Tamura、T.Masujima：““色谱视频检查的发展 - 概念和基础应用”（日语）”Jasco 报告。

S.Razee, A.Tamura, T.Masujima: ""Direct Observation of Capillary Electrophoresis Phenomena Using a Video Image Analysis system"" Chem.Lett.1996. 93-94 (1996)

S.Razee、A.Tamura、T.Masujima：“使用视频图像分析系统直接观察毛细管电泳现象”Chem.Lett.1996。