Development of Dual-Tandem-Mass Spectrometer with a New Ion-trap and Its Application to Analysis of Molecular Nano-dynamics of a Cell

新型离子阱双串联质谱仪的研制及其在细胞分子纳米动力学分析中的应用

• 批准号: 18390014
• 负责人: MASUJIMA Tsutomu
• 金额: $ 10.27万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390014/
• 关键词: Mass Spectrometer; Ion Trap; Cell Molecular Analysis; Time of Flight Mass Spectrometer; Quadrupole Mass Spectroteter

1. Development of Dual-Tandem-Mass Spectrometer with New Ion-trapA new concept of tripole was simulated by computer and it was found that this new tripole can be used not only for mass filter but also for a new gap ion trap which consists of two tripoles. With these new ion optics, a new dual tandem mass spectrometer which has two mass filter, one collision cell and one time-of-flight mass spectrometer, by which we can analyze molecules in quantitative and qualitative ways. The test machine was developed and its performance was checked.2. Live Single Cell Mass Spectrometric AnalysisWith such sensitive and versatile new mass spectrometer, we can apply this type of mass spectrometer for live single cell analysis. However, the molecular amount of a single cell is very tiny with a million molecules for each molecular species. Thus it has not been succeeded before. We finally found a new super sensitive ionization methodology in which we inserted a nano-electro spray tip into a single cell under video-microscope and suck the contents of a cell and the tip was directly used as a nano-electro spray ionization source. This method is so successful to introduce the molecular contents of a cell and to detect hundreds of molecular peaks.This new method will be used in every aspect of bio-sciences and will accelerate the speed of analyses of life sciences.

1.新型离子阱双串联质谱仪的研制对一种新的三极概念进行了计算机模拟，发现这种新的三极不仅可以用于滤质器，而且可以用于由两个三极组成的新型间隙离子阱。利用这些新的离子光学器件，我们设计了一种新型的双串联质谱仪，它包括两个滤质器、一个碰撞池和一个飞行时间质谱仪，可以对分子进行定量和定性分析。研制了试验机，并对其性能进行了检验.活单细胞质谱分析有了这种灵敏度高、功能多的新型质谱仪，我们可以将这种类型的质谱仪应用于活单细胞分析。然而，单个细胞的分子量非常小，每个分子种类有一百万个分子。因此以前没有成功过。我们最终发现了一种新的超灵敏电离方法，即在视频显微镜下将纳米电喷雾尖端插入单个细胞中，吸取细胞内容物，并将尖端直接用作纳米电喷雾电离源。该方法成功地引入了细胞的分子组成，检测了数百个分子峰，将应用于生命科学的各个方面，加快了生命科学分析的速度。

项目成果

ビデオマススコープ法の開発:主成分分析によるダイレクトMS1細胞分子表現形分析

视频质量范围界定方法的发展：使用主成分分析直接进行 MS1 细胞分子表型分析

• 发表时间: 2008
• 作者: 関根 勇一;他;津山 尚宏
• 通讯作者: 津山 尚宏

Development of Video-mass scope ; application for single cell sub-cellular metabolomics

视频质量范围的开发；

• 发表时间: 2008
• 作者: H.;Mizuno;T.;Masujima;et. al.
• 通讯作者: et. al.

ディファレンシャルMSディスプレイ法による細胞内低分子変動分析

利用微分MS显示法分析细胞内小分子波动

• 发表时间: 2008
• 作者: Morita;Y.;et al.;山中 祐介;森山芳則;堀江 洋平
• 通讯作者: 堀江 洋平

Sensitive peptide sequencing for video-mass scope method

用于视频质谱法的灵敏肽测序

• 发表时间: 2007
• 作者: R.;Manabe;T.;Masujima;et. al.
• 通讯作者: et. al.

nano ESI-MS/MSによる低分子及びペプチド同定法の開発

使用纳米 ESI-MS/MS 开发小分子和肽鉴定方法

• 发表时间: 2007
• 作者: 紙谷浩之;福永賢輝;大山隆;原島秀吉;真鍋 領
• 通讯作者: 真鍋 領