Regulation of the S-phase CDK activity required for the DNA replication by Nik1 and Swe1 kinases in S.cerevisiae
酿酒酵母中 Nik1 和 Swe1 激酶对 DNA 复制所需的 S 期 CDK 活性的调节
基本信息
- 批准号:11680698
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In this research program, we have searched for novel cell cycle regulators in the budding yeast S.cerevisiae by cloning multicopy suppressors of the cdc2-L7 mutation of the fission yeast, S.pombe. One of the isolated genes encodes a putative protein kinase similar to Nim1 of S.pombe and was termed NIK1 (Nim1-like kinase 1). NIK1 is identical to HSL1, and the results obtained so far suggests that NIK1 is a nim1+homolog of S.cerevisiae. NIK1 has unique features as follows that have not been reported on nim1+ ; (1) the transcription of NIK1 was periodic with peaked at the G1/S boundary. (2) Ejnik1 cells showed HU and MMS sensitivity. (3) DNA was aberrantly distributed in the Ejnik1cdc28 double mutant. (4) Nik1 interacts with the Cdc28 complex genetically and biochemically. (5) NIK1 seemed to have genetic interaction with CDC6. These results suggests that Nik1 interacts with the Cdc28 complex and functions not only at the G2/M transition but also at S phase of the cell cycle. We analyzed on the phenotypes of nik1 Ej which were considered to be caused by some defects in DNA replication, i.e. HU sensitivity, higher plasmid instability and increased heat sensitivity in S-phase related ts mutant strains. These phenotypes were suppressed by swe1 Ej and Nik1 can phosphorylate Swe1, therefore, it was prospected that they were caused by the lowered activity of S phase Cdc28. Indeed, in vitro analysis of FLAG-tagged Clb5-dependent kinase activity revealed that the kinase activity of htis was inhibited by Swe1 as in the case of Clb2-dependent kinase. Moreover, cdc28Y19E mutant showed delayed initiation and progression of the DNA replication. Tyrosine-phosphorylated Cdc28 appears at S phase. These data indicate S-phase CDK complexes, at least including the Clb5-Cdc28 complex, is regulated by phosphorylation of conserved Tyrosine.
Please try later.
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ohtani K.et al.: "Cell-growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F"Oncogene. 18. 2299-2309 (1999)
Ohtani K.等人:“转录因子 E2F 介导的哺乳动物 MCM5 和 MCM6 基因的细胞生长调节表达”癌基因。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Fukushima,K., et al.: "Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination."Nuc.Acids Res.. 28. 2709-2714 (2000)
Fukushima,K., et al.:“粟酒裂殖酵母的 Dmc1 在减数分裂重组中发挥作用。”Nuc.Acids Res.. 28. 2709-2714 (2000)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Iguchi N.et al.: "Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis"FEBS Lett.. 456. 315-321 (1999)
Iguchi N.等人:“单倍体生殖细胞特异性 tektin cDNA 的分子克隆和小鼠睾丸中蛋白质的分析”FEBS Lett.. 456. 315-321 (1999)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tanaka,S., et al.: "Nik1 : a protein kinase of S.cerevisiae interacts with the the Cdc28 complex and regulates the cell cycle progression."Genes Cells. 1. 905-921 (1996)
Tanaka,S. 等人:“Nik1:酿酒酵母的蛋白激酶与 Cdc28 复合物相互作用并调节细胞周期进程。”Genes Cells。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Okuzaki,D., et al.: "Kcc4 associates with septin proteins of Saccharomyces cerevisiae."FEBS Lett.. 24555. 1-5 (2001)
Okuzaki,D., et al.:“Kcc4 与酿酒酵母的脓毒蛋白相关。”FEBS Lett.. 24555. 1-5 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
NOJIMA Hiroshi其他文献
NOJIMA Hiroshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('NOJIMA Hiroshi', 18)}}的其他基金
Development and application of a novel technique that allows analysis on the gene expression of a single cell.
开发和应用一种新技术,可以分析单个细胞的基因表达。
- 批准号:
21651085 - 财政年份:2009
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Functional analysis of the kinase complex that regulates the connection between the centrosome cycle and M phase.
对调节中心体周期和 M 期之间连接的激酶复合物进行功能分析。
- 批准号:
20370081 - 财政年份:2008
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development and application of nano-subtraction technique
纳米减影技术的发展及应用
- 批准号:
15101006 - 财政年份:2003
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
EFFECT OF PLANT HORMONE ON SEED DEVELOPMENT IN PEANUT (Arachis hypogaea L.)
植物激素对花生种子发育的影响(ArachishypogaeaL.)
- 批准号:
12660011 - 财政年份:2000
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Comprehensive isolation and functional analysis of the genes whose expressions are specifically induced upon entry into the Go phase of cell cycle
对进入细胞周期 Go 期时特异性诱导表达的基因进行全面分离和功能分析
- 批准号:
12794010 - 财政年份:2000
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for University and Society Collaboration
Systematic isolation and analysis of novel genes that control cell cycle and cell growth.
系统分离和分析控制细胞周期和细胞生长的新基因。
- 批准号:
08458220 - 财政年份:1996
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development and application of ESS method
ESS方法的开发与应用
- 批准号:
07558216 - 财政年份:1995
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Cloning and functional analusis of cell-cycle regulatory genes by complementation cloning.
通过互补克隆进行细胞周期调控基因的克隆和功能分析。
- 批准号:
05454647 - 财政年份:1993
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Cloning and analysis of the genes involved in the regulation of cell cycle and growth by complementation cloning.
通过互补克隆对参与细胞周期和生长调节的基因进行克隆和分析。
- 批准号:
02454537 - 财政年份:1990
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Development and application of a new vector system for the preparation of the whole catalog of a human cDNA library.
一种新载体系统的开发和应用,用于制备人类 cDNA 文库的整个目录。
- 批准号:
02557098 - 财政年份:1990
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
相似国自然基金
外源氨基酸干预H.uvarum与S.cerevisiae混菌发酵产乙酸高级醇酯的机制研究
- 批准号:32001717
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Epigenetic transmission in S.cerevisiae: links to DNA replication and cell cycle regulation
酿酒酵母的表观遗传传递:与 DNA 复制和细胞周期调控的联系
- 批准号:
RGPIN-2020-03874 - 财政年份:2022
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Epigenetic transmission in S.cerevisiae: links to DNA replication and cell cycle regulation
酿酒酵母的表观遗传传递:与 DNA 复制和细胞周期调控的联系
- 批准号:
RGPIN-2020-03874 - 财政年份:2021
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Epigenetic transmission in S.cerevisiae: links to DNA replication and cell cycle regulation
酿酒酵母的表观遗传传递:与 DNA 复制和细胞周期调控的联系
- 批准号:
RGPIN-2020-03874 - 财政年份:2020
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms of epigenetic conversions in S.cerevisiae
酿酒酵母表观遗传转化机制
- 批准号:
RGPIN-2015-06727 - 财政年份:2019
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms of epigenetic conversions in S.cerevisiae
酿酒酵母表观遗传转化机制
- 批准号:
RGPIN-2015-06727 - 财政年份:2018
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms of epigenetic conversions in S.cerevisiae
酿酒酵母表观遗传转化机制
- 批准号:
RGPIN-2015-06727 - 财政年份:2017
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms of epigenetic conversions in S.cerevisiae
酿酒酵母表观遗传转化机制
- 批准号:
RGPIN-2015-06727 - 财政年份:2016
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Mechanisms of epigenetic conversions in S.cerevisiae
酿酒酵母表观遗传转化机制
- 批准号:
RGPIN-2015-06727 - 财政年份:2015
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual
Epigenetic effects at the telomeres of S.cerevisiae
酿酒酵母端粒的表观遗传效应
- 批准号:
466905-2014 - 财政年份:2014
- 资助金额:
$ 2.37万 - 项目类别:
University Undergraduate Student Research Awards
Wine yeast genomics: adaptation of S.cerevisiae to fermentation stress
葡萄酒酵母基因组学:酿酒酵母对发酵应激的适应
- 批准号:
217271-2009 - 财政年份:2014
- 资助金额:
$ 2.37万 - 项目类别:
Discovery Grants Program - Individual