Molecular basis for highly pathogenesis of avian influenza virus in humans.

禽流感病毒在人类中高度致病的分子基础。

• 批准号: 12307008
• 负责人: KAWAOKA Yoshihiro
• 金额: $ 31.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307008/
• 关键词: avian influenza virus; H5N1; reverse genetics; インフルエンザウイルス; リバース・ジェネティクス; 病原性; 香港; ヘマグルチニン; HA; 宿主域; 組織指向性

Influenza is an example of a disease for which the viral pathogen has emerged into the human population many times over past centuries, sometimes with devastating consequencesIn 1997, an H5N1 influenza A virus was transmitted from birds to humans in Hong Kong, killing 6 out of the 18 people infected. When mice were infected with human isolates, two virulence groups became apparent. Using reverse genetics, we showed that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice. Moreover, high cleavability of the hemagglutinin glycoprotein was essential requirement for lethal infection.

流感是一种病毒病原体在过去几个世纪中多次出现在人类中的疾病，有时会造成毁灭性的后果。1997年，H5N1甲型流感病毒在香港由鸟类传播给人类，18名感染者中有6人死亡。当小鼠感染人类分离株时，两个毒力组变得明显。使用反向遗传学，我们发现PB2蛋白中627位的突变影响了小鼠感染的结果。此外，血凝素糖蛋白的高裂解性是致死性感染的基本要求。

项目成果

Human influenza A viral genes responsible for the restriction of its replication in duck intestine

• DOI: 10.1006/viro.2002.1358
• 发表时间: 2002-04-10
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Hatta, M;Halfmann, P;Kawaoka, Y
• 通讯作者: Kawaoka, Y

Hatta M, Halfmann P, Wells K, Kawaoka Y.: "Human influenza a viral genes responsible for the restriction of its replication in duck intestine"Virology. 295(2). 250-255 (2002)

Hatta M、Halfmann P、Wells K、Kawaoka Y.：“人类流感病毒基因负责限制其在鸭肠中的复制”病毒学。

Hughes MT., McGregor M., Suzuki T., Suzuki Y., Kawaoka Y.: "Adaptation of influenza A viruses to cells expressing low levels of sialic acid leads to loss of neuraminidase activity"Journal of Virology. 75. 3766-3770 (2001)

Hughes MT.、McGregor M.、Suzuki T.、Suzuki Y.、Kawaoka Y.：“甲型流感病毒适应表达低水平唾液酸的细胞会导致神经氨酸酶活性丧失”《病毒学杂志》。

Schultz-Cherry S., Dybdahl-Sissoko N., Neumann G., Kawaoka Y., Hinshaw VS.: "Influenza Virus NS1 Protein Induces Apoptosis in Cultured Cells"Journal of Virology. 75. 7875-7881 (2001)

Schultz-Cherry S.、Dybdahl-Sissoko N.、Neumann G.、Kawaoka Y.、Hinshaw VS.：“流感病毒 NS1 蛋白诱导培养细胞凋亡”病毒学杂志。

Kobasa D., Wells K., Kawaoka Y.: "Amino Acids Responsible for the Absolute Sialidase Activity of the Influenza Virus Neuraminidase : Relationship to Growth in Duck Intestine"Journal of Virology. 75. 11773-11780 (2001)

Kobasa D.、Wells K.、Kawaoka Y.：“负责流感病毒神经氨酸酶绝对唾液酸酶活性的氨基酸：与鸭肠生长的关系”病毒学杂志。