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General investigation of malocclusion in the aspect of age-related expression mechanism

错牙合畸形年龄相关表达机制的普查

基本信息

批准号：
12307050
负责人：
SOMA Kunimichi
金额：
$ 23.17万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307050/
关键词：
aging malocclusion periodontium alveolar bone

项目摘要

Extracellular matrixOcclusal hypofunction of rat unilateral mandibular molars was induced by extraction of the opposing teeth, and immunohistochemical analyzes were performed to clarify the influence of occlusal hypofunction on the changes of proteoglycan content in the periodontal ligament.proteoglycan decreased in the periodontal ligament due to loss of occlusal function, and these results suggest that proteoglycans are closely related to occlusal function and play important roles in tissue homeostasis or tissue remodeling.MicrovasculatureWe examined the effect of occlusal stimuli on the microvasculature of the periodontal ligament using a model of occlusal hypofunction in rat molars. The occlusal hypofunction caused narrowing of the periodontal membrane and reduction in the vessel size with decrease in the number of blood vessles. In this study, a mechanism is revealed that hypofunction would result in increase of the expression of endothelin, a potent vasoconstrictive factor, furth … More ermore it also suggested the relation to expression of nitric oxide synthase(NOS), a vasodilator factor.Periodontal mechanoreceptorsThe neurophysiological properties of the periodontal mechanoreceptors were studied using an in vitro jaw-nerve preparation. Results of this study have demonstrated the effects of experimental occlusal hypofunction on the periodontal mechanical signaling. Moreover, changes related to growth and development have also been investigatedActivity of bone formationIn order to clarify the mechanism of bone remodeling under mechanical stress, cultured osteoblastic-cells were subjected to shear stress, in which we have revealed that the enhancement of intracellular Ca^<2+> depended on the property of stretch-activated channel and were modulated by the cytoskeleton such as actin filament.We also elucidated the hypofunctional condition of the root, remodeling of the alveolar bone and the physiological tooth movement in rats. And, the experimental tooth movements under hypofunctional condition have made it more obvious that light orthodontic forces contribute to the conservation of sound periodontal tissue. Less

细胞外基质拔除大鼠一侧下颌磨牙后，建立咬合功能低下模型，应用免疫组织化学方法观察咬合功能低下对牙周膜蛋白多糖含量变化的影响。咬合功能丧失导致牙周膜蛋白多糖含量下降，提示蛋白多糖与咬合功能密切相关，在组织动态平衡或组织重建中发挥重要作用。咬合功能减退导致牙周膜变窄，血管管径缩小，血管数量减少。本研究揭示了功能减退导致强大的血管收缩因子…表达增加的机制。此外，它还提示与血管扩张因子一氧化氮合酶(NOS)的表达有关。牙周机械感受器用体外颌神经标本研究牙周机械感受器的神经生理学特性。本研究结果证实了实验性咬合功能减退对牙周力学信号的影响。此外，我们还研究了与生长发育相关的变化和骨形成的活性。为了阐明机械应力下骨重建的机制，我们对培养的成骨细胞施加剪应力，揭示了细胞内钙的增强依赖于拉伸激活通道的特性，并受到肌动蛋白微丝等细胞骨架的调节。我们还阐明了大鼠牙根功能减退、牙槽骨改建和生理性牙齿移动。而且，在功能低下的情况下进行的实验性牙齿移动更加明显地表明，轻微的正畸力有助于保护健康的牙周组织。较少