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Tissue Engineering of the Periodontal Ligament Stimulated by Orthodontic Treatment

正畸治疗刺激牙周膜的组织工程

基本信息

批准号：
14370688
负责人：
SOMA Kunimichi
金额：
$ 8.83万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370688/
关键词：
occlusal hypofunction periodontal ligament orthodontic force recovery regeneration transplantation root resorption

项目摘要

AIM: In our study using rat occlusal hypofunction model, we have reported the changes in width of the periodontal ligament (PDL), the number and the size of blood vessels, distribution of the extracellular matrix, and the distribution and the morphology of mechanoreceptors. In this study, we applied various mechanical stresses on hypofunctional tooth in this model to clarify the mechanism of recovery in the function of periodontal ligament. Further, we also examined the possibility to regenerate the periodontal ligament stimulated by orthodontic forces.MATERIALS AND METHODS: Occlusal hypofunctional rat model was prepared by extracting molars or setting bite-plate on incisors. Occlusal force or 2-10 gf orthodontic force was continuously or intermittently applied to the hypofunctional teeth. Distribution of the extracellular matrix and Nitric Oxide synthase, tooth movement and root resorption was observed histochemically and immunohistochemically.RESULTS: The distance of the tooth movement in the hypofunctional model was different from normal model. Root resorption was induced when the strong force were applied to the hypofunctional teeth. It was suggested that lighter force could move teeth smoothly without inducing pathological changes. Distribution and synthesis of the extracellular matrix and nitric oxide synthase was influenced by mechanical stress, to maintain the function of the PDLCONCLUSION: The effect of mechanical stress on periodontal ligament of the. hypofunctional tooth was different from the normal tissue. Therefore strength, type and/or duration of the force must be changed to avoid pathological change such as root resorption. However, mechanical stress such as occlusal force or orthodontic force can recover atrophically changed PDL, which suggest that appropriate mechanical stimuli are useful in recovering and regenerating PDL.

目的：采用大鼠咬合功能减退模型，观察大鼠牙周韧带宽度、血管数量和大小、细胞外基质分布、机械感受器分布和形态的变化。在本研究中，我们将不同的机械应力施加于功能缺失牙的模型上，以阐明牙周韧带功能恢复的机制。此外，我们也研究了在正畸力刺激下牙周韧带再生的可能性。材料与方法：拔除磨牙或在门牙上设置咬合板制备咬合功能低下大鼠模型。对功能障碍牙连续或间歇施加咬合力或2 ~ 10gf正畸力。细胞外基质和一氧化氮合酶的分布、牙齿运动和牙根吸收的组织化学和免疫组织化学观察。结果：功能障碍模型牙体运动距离与正常模型有明显差异。对功能不全的牙施加强力，可诱导牙根吸收。提示较轻的力可以使牙齿运动平稳而不引起病变。机械应力影响牙周膜细胞外基质和一氧化氮合酶的分布和合成，维持牙周膜的功能。功能缺损牙与正常组织不同。因此，必须改变力的强度、类型和/或持续时间，以避免病理性变化，如牙根吸收。然而，机械应力如咬合力或正畸力可以恢复萎缩改变的PDL，这表明适当的机械刺激对恢复和再生PDL是有用的。