Horticultural utilization of self-incompatibility gene and its products in the Japanese pear.

日本梨自交不亲和基因及其产物的园艺利用.

• 批准号: 12660023
• 负责人: HIRATSUKA Shin
• 金额: $ 2.3万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660023/
• 关键词: Japanese pear; self-incompatibility; S-RNase; S-gene; Sタンパク質

Results on relationships between self-incompatibility expression and S-RNase concentrations in the style of Japanese pear cultivars were summarized and presented at "The International Symposium on Asian Pears" held in the summer of 2001. The content of the Symposium is in press as a review in Acta Horticulturae, and detailed data are in press in Sci. Hort.S-RNase was purified from pear styles and subjected to pollen assay in vitro ; S-RNase selectively inhibited the incompatible pollen germination and tube growth, and the inhibition was dose-dependent. These results support the hypothesis described above that cultivar difference in self-incompatibility expression is regulated by the S-RNase concentration in the style of each cultivar.Specific amplification of S_<1->, S_<2->, S_<4->, and S_5-gene fragments was succeeded. By using this technique, S-genotypes of several cultivars were re-examined, and plants with new S-genotypes were identified in F_1 plants produced in our laboratory. These results were published in J. Japan. Soc. Hort. Sci. Meanwhile, S_2-gene fragment was used as a probe and S_2-gene expression was compared among several cultivars possessing S_2-gene. Gene expression was considerably different among the cultivars, and the difference seemed parallel with S_2-RNase concentration in each cultivar. These results suggest that cultivar differences in self-incompatibility expression are due to different expression of S-gene in respective cultivars.

总结了日本梨品种自交不亲和性表达与花柱中S-RNase浓度关系的结果，并在2001年夏季举行的“亚洲梨国际研讨会”上发表。研讨会的内容作为评论发表在《园艺学报》上，详细的数据发表在《科学》上。霍特.从梨花柱中分离纯化S-RNase，并进行离体花粉测定，结果表明S-RNase选择性抑制不亲和花粉萌发和花粉管生长，且抑制作用呈剂量依赖性。这些结果支持了品种间自交不亲和性表达的差异受品种花柱中S-RNase浓度的调节的假说<1-><2-><4->。利用该技术对几个品种的S基因型进行了重新检测，并在本实验室生产的F_1植株中鉴定出了新的S基因型植株。这些结果发表在J. Japan上。霍特科学社同时，以S_2基因片段为探针，比较了几个具有S_2基因的品种的S_2基因表达。不同品种间的基因表达差异较大，且这种差异与S_2-RNase浓度的高低呈平行关系。这些结果表明，自交不亲和性表达的品种差异是由于S基因在各自品种中的不同表达。

项目成果

S.Hiratsuka, S-L.Zhang: "Cultivar differences in the expression of self-incompatibility in Japanese pears"Acta. Hort.. (in press).

S.Hiratsuka，S-L.Zhang：“日本梨自交不亲和性表达的品种差异”学报。

S.Hiratsuka, S-L.Zhang: "Relationships between fruit set, Pollen-tube growth, and S-RNase concentration in the self-incompatible Japanese pear"Sci. Hort.. (in press).

S.Hiratsuka，S-L.Zhang：“自交不亲和日本梨的坐果、花粉管生长和 S-RNase 浓度之间的关系”Sci。

平塚伸 他4名: "ニホンナシ'長寿','君塚早生','明月'および'市原早生'の自家不和合性遺伝子型の再考"園芸学会雑誌. 70・3(印刷中). (2001)

Shin Hiratsuka 等 4 人：“重新考虑日本梨‘长寿’、‘Kimizuka Wase’、‘Meigetsu’和‘Ichihara Wase’的自交不亲和基因型”，日本园艺学会杂志 70, 3（出版中）。 （2001）

S.Hiratsuka et al.: "Selective inhibition of the growth of incompatible Pollen tubes by s-protein in the Japanese pear"Sex. Plant Reprod.. 13. 209-215 (2001)

S.Hiratsuka 等人：“日本梨中 s 蛋白对不相容花粉管生长的选择性抑制”。

S-L.Zhang, S.Hiratsuka: "Cultivar and developmental differences in s-protein concentration and self-incompatibility in the Japanese pears"Hort Science. 35. 917-920 (2000)

S-L.Zhang，S.Hiratsuka：“日本梨中 s 蛋白浓度和自交不亲和性的品种和发育差异”Hort Science。