Effects of endocrine disruptors on female rat reproductive function

内分泌干​​扰物对雌性大鼠生殖功能的影响

• 批准号: 12836009
• 负责人: OHTA Yasuhiko
• 金额: $ 1.54万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12836009/
• 关键词: Endocrine disruptors; Rat; Uterus; Inplantation; Hormone receptor

Progesterone and estrogen receptor (PR and ER) expressions were examined immunohistochemically in female rats exposed to andogen and bisphenol-A (BPA) to evaluate effect of endocrine disrupter on female reproductive function.PR and ER expressions following a series of hormonal regime after ovariectomy as adults were affected in persistent estrous rats given andogen neonatally. Changes in expressions of EGF, TGF-α and EGF receptor (EGF-R) in the endometrial cells after the treatment were less evident compared with those in the PR and ER expressions.Adult rats given BPA for 7 day from the day of bith showed regular estrous cycles on day 60 and their ovaries contained follicles and corpora lutea. In BPA-treated rats, however, the ovaries were significantly smaller than in the controls. The uteri were markedly heavier in these BPA-treated rats than in the controls. Uterine response to the ovarian hormones was essentially the same between BPA-treated and control rats.These findings indicate that steroid hormone receptor is a potent marker for evaluation of endocrine disruptor in female reproduction, and that neonatal exposure of the female rat to BPA has some effects on both the central nervous system controlling female reproduction and peripheral organs.

为探讨内分泌干扰物对雌性大鼠生殖功能的影响，用免疫组织化学方法检测了雌激素受体（PR和ER）的表达，结果表明，雌激素受体（PR和ER）的表达与雌激素受体（PR和ER）的表达有关。BPA处理后子宫内膜细胞EGF、TGF-α和EGF受体（EGF-R）表达的变化较PR和ER表达的变化小，BPA处理7 d后，60 d时大鼠的动情周期较正常，卵巢中有卵泡和黄体。然而，在BPA处理的大鼠中，卵巢明显小于对照组。这些BPA处理的大鼠的子宫明显比对照组重。这些结果表明，类固醇激素受体是评价雌鼠生殖内分泌干扰物的有效标志物，新生期暴露于BPA的雌鼠对控制雌鼠生殖的中枢神经系统和外周器官均有一定的影响。

项目成果

Yamamura, Y. et al.: "Metallothionein expression and tumor progression in transplantable pregnancy-independent mouse mammary tumors"J. Vet. Med. Sci.. 63. 687-689 (2001)

Yamamura, Y. 等人：“可移植的妊娠非依赖性小鼠乳腺肿瘤中的金属硫蛋白表达和肿瘤进展”J.

Yamamura, Y. et al.: "Metallothionein expression and apoptosis in pregnancy dependent and -independent mouse mammary tumors"Anticancer Res.. 21. 1145-1150 (2001)

Yamamura，Y.等人：“妊娠依赖性和非依赖性小鼠乳腺肿瘤中的金属硫蛋白表达和细胞凋亡”Anticancer Res.. 21. 1145-1150 (2001)

Yamamura,Y et al.: "Metallothionein expression in transplantable mouse mammary tumors."Anticancer Res.. 20. 397-384 (2000)

Yamamura,Y 等人：“可移植小鼠乳腺肿瘤中的金属硫蛋白表达。”Anticancer Res.. 20. 397-384 (2000)

Okada, A. et al.: "Effect of diethylstilbestrol on cell proliferation and expression of epidermal growth factor in the developing female rat reproductive tract"J.Endocri.. 170. 539-554 (2001)

Okada, A. 等人：“己烯雌酚对雌性大鼠生殖道发育中细胞增殖和表皮生长因子表达的影响”J. Endocri.. 170. 539-554 (2001)

Yamamura,Y et al.: "Metallothionein expression and apeplosis in pregnancy-dependent and-independent mouse mammary tumors"Anticancer Res.. (in press).

Yamamura,Y 等人：“妊娠依赖性和独立性小鼠乳腺肿瘤中的金属硫蛋白表达和细胞凋亡”抗癌研究（正在出版）。