Autologe Translokation von Irispigmentepithel auf resorbierbaren Unterlagen als neue therapeutische Methode bei altersbedingter Makuladegeneration (Autologous iris pigment epithelium (IPE) translocation on biodegradable supports as new therapeutic modalit

虹膜色素上皮(IPE)细胞移位是治疗老年性黄斑变性的一种新的治疗方法，作为手术切除新生血管复合体以替代退化的色素细胞后的辅助治疗。IPE细胞移植必须具有与原始RPE细胞相同的形态结构才能具有完全的功能，即它们必须形成一个完整的单层。因此，将IPE细胞作为已在单层中的一层细胞进行移植，或者通过共移植基底膜材料来促进IPE细胞悬液在老化和损伤的Bruchs膜存在下的细胞黏附是至关重要的。尽管移植一片细胞在手术上是困难的，但这不仅可以确保细胞处于单层，而且可以更好地在黄斑下移位和定位，并提供新的基底膜材料。这项提案有两个主要部分，将作为四个研究中心的合作努力开发：一个部分涉及开发自然支持和将这些片材上的IPE单层移植到视网膜下空间。这一部分介绍了一种开发生物可降解膜的程序，该膜将支持自体IPE的单层生长和功能。第二部分将重点介绍液体人工可生物降解聚合物凝胶的发展。这些富含细胞外基质蛋白的凝胶将用于培养自体IPE细胞，然后将其注入视网膜下腔。这将促进色素上皮对裸露或缺失的Bruch膜的再生。将进行广泛的体外和体内测试，以确定最适合未来人类应用的技术。

Iris pigment epithelial (IPE) cell translocation represents a new treatment modality of age related macular degeneration as adjuvant therapy after surgical removal of the neovascular complex to replace the degenerated pigment cells. IPE cell transplants must assume the same morphological structure as the original RPE cells to be fully functional, namely they must form an intact monolayer. Therefore, it is critical that IPE cells are transplanted either as a sheet of cells already in a monolayer or that cell adhesion of IPE cell suspensions is facilitated in the presence of aged and injured Bruch's membrane by co-transplantation of basal membrane material. Although transplantation of a sheet of cells is surgically difficult, this will not only assure that the cells will be in a monolayer, but it will allow better translocation and positioning under the macula and prvides new basal membrane material. This proposal has two main parts which will be developed as the collaborative effort of four study centers: One part involves the development of natural supports and transplantation of IPE monolayers on these sheets into the subretinal space. This part lead to a procedure for developing biodegradable membranes which will support the monolayer growth and function of autologous IPE. The second part will focus on the development of liquid artificial biodegradable polymeric gels. These gels enriched with extracellular matrix proteins will be used to culture autologous IPE cells which will then be injected into the subretinal space. This should enhance repopulation of denuded or missing Bruch's membrane by pigment epithelium. Extensive in vitro and in vivo testing will be performed in order to determine the most sutable technique for future human application.