Expression of NF-Y in cancer tissue and its relation to cell proliferation

癌组织中NF-Y的表达及其与细胞增殖的关系

• 批准号: 12670185
• 负责人: TANIMOTO Akihide
• 金额: $ 1.34万
• 依托单位: Okinaka Memorial Institute for Medical Research (2002)University of Occupational and Environmental Health, Japan (2000-2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670185/
• 关键词: cancer; NF-Y; Cell proliferation

The expression of NF-Y protein was detected in the cancer tissues of the breast, prostate, and stomach using a catalyzed signal amplification system for immunohistochemistry. Especially, gastric cancer cells showed a higher ratio of NF-Y expression in their nuclei, and also those of normal gastric epithelial in proliferative phase. The immunolocalization of proliferating nuclear antigen and MIB-1 were very consist with that of NF-Y. This suggested that the NF-Y is a potential new maker for gastric cancer cell proliferative activity. The cdc2 expression, which is regulated by NF-Y, was not detected in the cancer cells, possibly due to the antibody specificity we used for immunostain.The cdc2 gene promoter activity in cultured human gastric cancer cells was reduced by dominant negative NF-Y.Taken together, we showed that the NF-Y regulates the cdc2 gene promoter activity and that its expression is high in gastric cancer cell.

采用免疫组化催化信号放大系统检测乳腺癌、前列腺癌和胃癌组织中NF-Y蛋白的表达。尤其是胃癌细胞核中NF-Y的表达比例较高，增殖期正常胃上皮细胞核中NF-Y的表达比例也较高。增殖核抗原和MIB-1的免疫定位与NF-Y的免疫定位非常一致。提示NF-Y是一种潜在的胃癌细胞增殖活性的新标记物。在胃癌细胞中未检测到受NF-Y调控的cdc 2基因的表达，这可能是由于我们用抗体特异性进行免疫染色的缘故，而在培养的人胃癌细胞中，cdc 2基因启动子的活性被显性负性NF-Y抑制。