Investigation into the roles of autoantibodies in the pathogenesis of central nervous system lupus erythematosus.

我们检测了抗核糖体P蛋白抗体（anti-P）在人免疫能力细胞表面识别的表位的存在，以描述其在中枢神经系统狼疮发病机制中的作用。用亲和纯化的抗p IgG对正常健康人CD4+和GD8+ T细胞、单核细胞和B细胞进行反应，并用fitc偶联的F（ab’）_2山羊抗人IgG片段进行染色，用碘化丙烯染色进行流式细胞术检测活细胞。在新鲜cd4 +和CD8+ T细胞、单核细胞或新鲜B细胞表面未发现与核糖体P蛋白耳甲末端22氨基酸序列具有抗原性相关的表位。然而，核糖体P表位在CD4+和CD8+ T细胞上被固定化抗cd3激活后，在单核细胞上被不含IFN-γ的on孵卵24小时后诱导表达，而在活化的B细胞上不表达。这些结果表明，抗p是一种抗淋巴细胞抗体，可与活化的T细胞和单核细胞发生反应，而不与静止的T细胞、单核细胞或B细胞发生反应，提示抗p可能对系统性红斑狼疮免疫失调有直接作用。狼疮精神病患者脑脊液中抗谷氨酸受体抗体未见明显升高，提示其为抗神经元抗体识别。

We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells in order to delineate its role in the pathogenesis of CNS lupus. Highly purified CD4+ and GD8+ T cells, monocytes, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab')_2, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the earboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface offreshCD4+ and CD8+ T cells, monocytes, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4+ and CD8+ T cells after activation with immobilized anti-CD3 and on monocytes after 24h incubation with on without IFN-γ, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte' antibody, which reacts with activated T cells and monocytes but not with resting T cells, monocytes or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus. We could not detect significant elevation of anti-glutamate receptor antibody in cerebrospinal fluid from patients with lupus psychosis, indicating that recognized by anti-neuronal antibody.