Investigation into the roles of autoantibodies in the pathogenesis of central nervous system lupus erythematosus.

自身抗体在中枢神经系统红斑狼疮发病机制中作用的研究。

基本信息

批准号：
12670438
负责人：
HIROHATA Shunsei
金额：
$ 2.18万
依托单位：
Teikyo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells in order to delineate its role in the pathogenesis of CNS lupus. Highly purified CD4+ and GD8+ T cells, monocytes, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab')_2, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the earboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface offreshCD4+ and CD8+ T cells, monocytes, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4+ and CD8+ T cells after activation with immobilized anti-CD3 and on monocytes after 24h incubation with on without IFN-γ, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte' antibody, which reacts with activated T cells and monocytes but not with resting T cells, monocytes or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus. We could not detect significant elevation of anti-glutamate receptor antibody in cerebrospinal fluid from patients with lupus psychosis, indicating that recognized by anti-neuronal antibody.

我们检查了人类免疫能力细胞的细胞表面上通过抗体P蛋白抗体（抗P）识别的表位，以描绘出其在CNS狼疮的发病机理中的作用。高度纯化的CD4+和GD8+ T细胞，来自正常健康个体的单核细胞和B细胞与亲和纯化的IgG抗P反应，并用FITC结合的F（AB'2（AB'）染色，碎片山羊goat抗抗人类IgG，然后对可为卵形细胞的流动性分析，可通过pepatiim iim imodim iodsed s sepans s s s s sepans s s s seplate cyterry，并在裂开中进行分析。与核糖体P蛋白的早期末端22-氨基酸序列相关的发作的存在未在表面OffRESHCD4+和CD8+ T细胞，单核细胞或新鲜B细胞上证明。然而，用固定的抗CD3激活后，核糖体P发作的表达在CD4+和CD8+ T细胞上诱导，并在24小时与无IFN-γ孵育后的单核细胞上诱导了单核细胞，而单核细胞在没有IFN-γ的情况下进行，而表位并未在活化的B细胞上表达。这些结果表明，抗P是一种抗大细胞抗体，它与活化的T细胞和单核细胞反应，但与静息T细胞，单核细胞或B细胞无反应，这表明抗P对全身性红lupus lupus eRythematosus中免疫失调的直接影响可能直接影响。我们无法检测到狼疮性精神病患者的脑脊液中抗谷氨酸受体抗体的显着升高，这表明抗神经元抗体识别。