Cell Cycle Dependence of Boron Uptake in Boron Compounds Used for Clinical Neutron Capture Therapy

用于临床中子捕获治疗的硼化合物中硼吸收的细胞周期依赖性

• 批准号: 12670852
• 负责人: YOSHIDA Fumiyo
• 金额: $ 1.09万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670852/
• 关键词: cell cycle; flow cytometry; sodium borocaptate; boronophenylalanine; neutron capture therapy; cell cycle

In neutron capture therapy, it is important to incorporate boron in tumor cells selectively. The present study used flow cytometry to sort the cells by G0/G1 phase and G2/M phase, and the boron concentration of each fraction was measured with ICP-AES. The results revealed that BSH and BPA, had higher rates of boron uptake in the G2/M than in the G0/G1. However, the difference was more prominent with BPA. The G2/M : G0/G1 ratio decreased as a function of time in culture in BPA, thereby indicating the cell cycle dependency of BPA uptake. Such heterogeneity of boron uptake in tumor cells should be considered for microdosimetry.

在中子俘获治疗中，选择性地将硼掺入肿瘤细胞中是重要的。本研究采用流式细胞仪对细胞进行G 0/G1期和G2/M期分选，并采用ICP-AES测定各组分中硼浓度。结果表明，BSH和BPA在G2/M中的硼吸收速率高于G 0/G1。然而，BPA的差异更为突出。G2/M：G 0/G1比值随着BPA培养时间的变化而降低，从而表明BPA摄取的细胞周期依赖性。肿瘤细胞硼吸收的这种异质性应考虑微剂量学。

项目成果

Yoshida, F.Matsumura, A., Shibata, Y., Okumura, M., et al.: "Cell cycle dependence of boron uptake in two boron compounds used clinical neutron capture therapy"Cancer Letters. (submitted).

Yoshida, F.Matsumura, A.、Shibata, Y.、Okumura, M.等人：“临床中子捕获疗法中使用的两种硼化合物中硼摄取的细胞周期依赖性”癌症快报。

Matsumura, A., Shibata, Y., Yamamoto, T., Yoshida, F., et al.: "In vivo and in vitro uptake study of boronated porphyrins for neutron capture therapy"Frontiers in Neutron Capture Therapy. 1015-1020 (2001)

Matsumura, A.、Shibata, Y.、Yamamoto, T.、Yoshida, F.等人：“用于中子捕获疗法的硼化卟啉的体内和体外摄取研究”中子捕获疗法的前沿。

Yamamoto, T., Matsumura, A., Shibata, Y., Yoshida, F., et al.: "Apoptosis of tumor cells in response to boron neutron capture therapy"Frontiers in Neutron Capture Therapy. 1293-1297 (2001)

Yamamoto, T.、Matsumura, A.、Shibata, Y.、Yoshida, F.等人：“硼中子捕获疗法响应肿瘤细胞凋亡”中子捕获疗法前沿。

Yoshida, F., Matsumura, A, Shibata, Y., et al.: "Cell cycle dependence of boron uptake in various boron compounds used for neutron capture therapy"Ninth International Symposium on neutron capture therapy for cancer Program and Abstracts. 57-58 (2000)

Yoshida, F.、Matsumura, A、Shibata, Y.等人：“用于中子捕获疗法的各种硼化合物中硼摄取的细胞周期依赖性”第九届国际癌症中子捕获疗法研讨会计划和摘要。

Shibata, Y., Matsumura, A., Yoshida, F., et al.: "Competitive uptake of porphyrin and LDL via the LDL receptor in glioma cell lines: flow cytometric analysis"Cancer Letters. 166. 79-87 (2001)

Shibata, Y.、Matsumura, A.、Yoshida, F. 等人：“神经胶质瘤细胞系中通过 LDL 受体竞争性摄取卟啉和 LDL：流式细胞术分析”Cancer Letters。