Experimental studies of the microangioarchitecture of tumors using monochromatic synchrotron radiation

单色同步辐射肿瘤微血管结构的实验研究

• 批准号: 12670913
• 负责人: IMAI Sigeki
• 金额: $ 2.18万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670913/
• 关键词: Synchrotron radiation; Monochromatic X-ray; Microangiography; Malingnant tumor; Tumor microangioarchitecture; 腫瘍血管; 新生血管

PURPOSE : To compare differences in the depiction of small vessels in tumors seen on microangiograms from a conventional soft X-ray system with those from a synchrotron radiation system and to evaluate the microangioarchitecture of these tumors and the growth of ne ovascularization.METHOD AND MATERIALS : VX2 carcinomas transplanted to the auricles of 15 rabbits randomized into three groups were investigated after one, three, and seven days. Five normal rabbits were the controls. Barium sulfate was used for fixation specimens. Microangiograms of auricle specimens were obtained both with a conventional soft X-ray system and a synchrotron radiation system. Non-ionic contrast media was used for in vivo study and microangiograms were obtained with a synchrotron radiation system.RESULTS : The conventional X-ray system could detect vessels with diameters of approximately l OOum, whereas the monochromatic synchrotron radiation system allowed depiction of microvessel of approximately 30um in di … More ameter. The size of the 1 -day-old tumor is approximately 2 mm. On the microangiogram, arteriovenous shunts and many radiating veins can be seen in the transplantation area. The 3-day-old tumor is approximately 5 mm. On the microangiograms, many irregular veins are present within tumor. The number of vessels is markedly increased. The size of the 7-day-old tumor is approximately 10 mm. On the microangiogram, a few irregular veins 30 um in diameter are present and many irregular ones can be seen along the periphery. Arteriovenous shunts are more numerous in the peripheral area than at day three. Tumor vessels displayed predominantly of venous flow.CONCLUSION : Synchrotron radiation microangiography is therefore considered a satisfactory method for observing the microangioarchitecture of malignant tumors in vivo. Synchrotron radiation microangiography in vivo offers the possibility of evaluating changes in tumor microvessel as small as 30 um in diameter after administration of anticancer drugs or angiogenesis inhibitors. Less

目得：为了比较从传统的软X射线系统与那些从同步辐射系统的微血管造影上看到的肿瘤中的小血管的描述的差异，并评估这些肿瘤的微血管结构和neovascularization.Method和MATERIALS的生长：VX 2癌移植到耳廓的15只兔子随机分为三组进行了研究后，一，三，七天。5只正常家兔为对照组。用硫酸钡固定标本。用常规软X线系统和同步辐射系统对耳廓标本进行微血管造影。结果：常规X线系统可显示直径约为100 μ m的微血管，而单色同步辐射系统可显示直径约为30 μ m的微血管。 ...更多信息 - 是的1天大的肿瘤大小约为2 mm。在微血管造影上，移植区可见动静脉分流和许多放射状静脉。3日龄的肿瘤约5毫米。在微血管造影上，肿瘤内有许多不规则的静脉。船只数量明显增加。7日龄肿瘤大小约10 mm，微血管造影可见少量直径约30 μ m的不规则静脉，周围沿着可见许多不规则静脉。周围区域的动静脉分流比第三天更多。结论：同步辐射微血管造影是一种较好的观察肿瘤血管构筑的方法。同步辐射微血管造影术在体内提供了评价肿瘤微血管的变化，直径小至30微米后，抗癌药物或血管生成抑制剂的管理。少

项目成果

Yamashita T: "Evaluation of the microangio archite cture of tumors by use of monochromatic x-rays"Investigative Radiology. 36(12). 713-720 (2001)

Yamashita T：“使用单色 X 射线评估肿瘤的微血管结构”研究放射学。

T.Yamashita: "Evaluation of the microangioarchitecture of tumors by use of monochromatic x-rays"Investigative Radiology. 36・12. 713-720 (2001)

T. Yamashita：“使用单色 X 射线评估肿瘤的微血管结构”36・12（2001 年）。

Umetani K et: "Synchrotron radiation microangiography using x-ray SATICON for observation of tumor"The Journal of The Institute of Image Information and television Engineers. 56(3). 492-494 (2002)

Umetani K 等人：“使用 X 射线 SATICON 观察肿瘤的同步辐射微血管造影”图像信息和电视工程师研究所杂志。

T.Yamashita: "Experimental studies of the microangioarchitecture of tumors using synchrotron radiation at Spring-8"Nuclear Instruments and Methods in Physics Research A. 467/468. 1346-1348 (2001)

T.Yamashita：“使用 Spring-8 同步加速器辐射进行肿瘤微血管结构的实验研究”物理研究中的核仪器和方法 A. 467/468。

梅谷啓二: "X線サチコンを使った放射光微小血管造影での腫瘍観察"映像情報メディア学会誌. 56・3. 492-494 (2002)

Keiji Umetani：“使用X射线同步辐射显微血管造影观察肿瘤”图像信息和电视技术研究所杂志56・3（2002年）。