The study for analgesic effects of Magnesium

镁的镇痛作用研究

• 批准号: 12671491
• 负责人: SUZUKI Masao
• 金额: $ 2.05万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671491/
• 关键词: Magnesium; Postoperative pain; Morphine; c-fos; Spinal cord dorsal horn; Histamine; Current Perception Threshold; 炎症性疼痛; ホリマリン

The effects of Magnesium on the postoperative pain in human, current perception threshold (CPT) in rat, and expression of c-fos protein in the spinal cord in rat evoked by histamine injection were studied.The changes in perioperative serum ionized Magnesium were investigated in 210 patients underwent elective operations. Serum ionized Magnesium was gradually decreased during operation and postoperative period. The decrease was greater in the patients with abdominal surgery than in those with orthopedic and neurosurgical operations. In 24 women undergoing elective abdominal hysterectomy, the effects of Magnesium sulfate administration on the postoperative analgesic requirements were studied. The amount of postoperative morphine in the Magnesium administrated group was smaller than control group.Male Wistar rats were allocated to six treatment groups : Gr 1(saline), Gr 2a (Magnesium 2mmol/kg), Gr 2b (Magnesium 4mmol/kg), Gr 3(morphine 2mg/kg), Gr 4a (morphine 2mg/kg + Magnesium 2mmol/kg), Gr4b (morphine 2mg/kg +Magnesium 4mmol/kg). Although CPT at 5Hz in Gr 3, 4a, and 4b increased, there were no intergroup differences.In another study, male Wistar rats were allocated to 4 treatment groups : Gr C (saline), Gr M1 (Magnesium 30mg/kg), Gr M5 (Magnesium 150mg/kg), Gr M10 (Magnesium 300mg/kg). Each rat then received an S.C. injection of histamine into the hind paw. Sections of the lumber spinal cord were immunostained with anti c-fos antibody ; the numbers of fos-like immunoreactive cells (FLI) in every specific lamina were counted. In the sections of L3 and L4 level, the numbers of FLI labeled neurons for Gr M5 and M10 were less than Gr C and M1.These findings suggested that Magnesium could be an interest adjuvant for postoperative pain management and have effects on spinal neurons, some of which directly receive noxious inputs.

观察镁对人术后疼痛、大鼠电流感觉阈值(CPT)和组胺诱发大鼠脊髓c-fos蛋白表达的影响，并观察210例择期手术患者围手术期血清离子镁的变化。血清离子镁在术中、术后逐渐降低。腹部手术患者的降幅比骨科和神经外科手术患者更大。在24例择期剖腹子宫切除术患者中，观察了硫酸镁对术后镇痛效果的影响。雄性Wistar大鼠随机分为6组：GR 1(生理盐水)、Gr 2a(镁2 mm o l/kg)、Gr 2b(镁4 mm o l/kg)、Gr 3(吗啡2 mg/kg)、Gr 4a(吗啡2 mg/kg+镁2 mm o l/kg)、Gr4b(吗啡2 mg/kg+镁4 mm o l/kg)。在另一项研究中，雄性Wistar大鼠被分成4个处理组：Gr C(生理盐水)、Gr M1(镁30 mg/kg)、Gr M5(镁150 mg/kg)、Gr M10(镁300 mg/kg)。然后，每只大鼠都被皮下注射组胺到后爪。取腰髓切片，用c-fos抗体进行免疫组织化学染色，计数各特定节段的Fos样免疫反应细胞(Fli)数。在L3和L4节段，GrM5和M10的FLI标记神经元数量少于GrC和M1。提示镁可能是术后疼痛处理的一种兴趣佐剂，并对部分直接接受伤害性输入的脊髓神经元产生影响。

项目成果

Shimada J, et al: "Change of ionized blood magnesium in perioperative period"J Anesth 13 Sippl : 199. (1999)

Shimada J 等人：“围手术期离子血镁的变化”J Anesth 13 Sippl：199。(1999)

島田二郎ほか: "マグネシウムの持続投与がアロディニアの軽減に有効であった帯状疱疹の一例"日本ペインクリニック学会誌. 7. 347 (2000)

Jiro Shimada 等人：“连续施用镁可有效缓解异常性疼痛的带状疱疹病例”日本疼痛临床医师学会杂志 7. 347 (2000)。

Shimada J, Murakawa M, Isomine S, Sato K, Aoki K: "Interaction of ionized magnesium and vecuronium in anesthetized patients"Anethesiology 91(3A) : A1041. (1999)

Shimada J、Murakawa M、Isomine S、Sato K、Aoki K：“麻醉患者中离子镁和维库溴铵的相互作用”麻醉学 91(3A)：A1041。

島田二郎ほか: "帝王切開術後鎮痛における硫酸マグネシウムの効果"日本ペインクリニック学会誌. 5. 369 (1998)

Jiro Shimada 等人：“硫酸镁对剖宫产术后镇痛的影响”日本疼痛临床学会杂志 5. 369 (1998)。

島田二郎ほか: "周術期におけるイオン化マグネシウムの変動"J Anesth. 13. S199 (1999)

Jiro Shimada 等人：“围手术期离子镁的变化”J Anesth 13. S199 (1999)