Functional Development of Nociceptors in the Oral Mucosa

口腔粘膜伤害感受器的功能发育

• 批准号: 12671800
• 负责人: TODA Kazuo
• 金额: $ 1.98万
• 依托单位: Nagasaki University (2001)Tokyo Medical and Dental University (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671800/
• 关键词: nociceptor; subtype; development; jaw-nerve preparation; high threshold; A-delta fiber; C-fiber; mechanonociceptor

Functional Development of the oral mucosal nociceptors was investigated using in vitro Jawnerve preparation which was recently developed by ours. A total of 36 male Wistar albino rats weighing 250-350 g were tested. The animals were housed in conventional acrylic cages with soft bedding under a 14 h light/10 h dark cycle. Only one recording was obtained from each animal and the animal was sacrificed immediately after experiment with an intracardiac injection of thiamylal sodium (100 mg/kg, Isozol^【○!R】, Yoshitomi Pharmacy, Osaka, Japan). All experiments were approved by the Animal Care Welfare Committee Nagasaki University.In the oral mucosa, four subtypes of nociceptors were found: A-delta high threshold mechanonociceptor, A-delta polymodal nociceptor, C high threshold mechanonociceptor, C polymodal nocieptor. These nociceptors were maturated by 3-5 weeks after birth. The ratio of polymodal nociceptors innervated by A-delta or C afferent fiber was decreased during development.

本实验采用我们新近研制的离体颌神经标本，研究了口腔粘膜伤害感受器的功能发育。共测试了36只体重为250-350 g的雄性Wistar白化病大鼠。将动物饲养在具有柔软垫料的传统丙烯酸笼中，14 h光照/10 h黑暗循环。从每只动物中仅获得一次记录，并且在实验后立即心内注射甲硫氨酰钠（100 mg/kg，Isozol）处死动物。R]，Yoshitomi Pharmacy，Osaka，Japan）。在口腔粘膜中发现4种伤害感受器亚型：A-δ高阈值机械感受器、A-δ多模态伤害感受器、C高阈值机械感受器、C多模态伤害感受器。这些伤害感受器在出生后3-5周成熟。在发育过程中，由A-δ或C传入纤维支配的多模态伤害性感受器的比例降低。

项目成果

Sato T, Toda K, Miyamoto T, Okada Y, Fujiyama R: "The origin of slow potentials on the tongue surface induced by frog glossopharyngeal efferent fiber stimulation"Chemical Senses. 25. 583-589 (2000)

Sato T、Toda K、Miyamoto T、Okada Y、Fujiyama R：“青蛙舌咽传出纤维刺激引起的舌头表面慢电位的起源”化学感官。

Toda K: "Nociceptors in the oral mucosa"Journal of Neurosciences for Pain Research. 2. 5-11 (2000)

Toda K：“口腔粘膜中的伤害感受器”疼痛研究神经科学杂志。

戸田一雄: "痛みのメカニズム-最近の神経生理学的知見より"日本歯科麻酔学会雑誌. 29. 89-94 (2001)

Kazuo Toda：“疼痛机制 - 来自最近的神经生理学发现”日本牙科麻醉学会杂志 29. 89-94 (2001)。

Toda K. et al.: "Gravity stimulation changes withdrawal reflex in rats"Grav. Space Biol. Bull.. 14. 28-31 (2000)

Toda K. 等人：“重力刺激改变了大鼠的撤退反射”Grav。

Toda K.: "Properties of nucleus raphe magnus neurons in relation to acupuncturte analgesia"Deutsch Ztschr Akup.. 44. 209-209 (2001)

Toda K.：“中缝大核神经元与针灸镇痛相关的特性”Deutsch Ztschr Akup.. 44. 209-209 (2001)