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Morphological analysis of the amygdalostriatal pathway in the control of emotional movements

控制情绪运动的杏仁纹状体通路的形态学分析

基本信息

批准号：
12680732
负责人：
TSUMORI Toshiko
金额：
$ 2.3万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680732/
关键词：
amygdala striatum substantia nigra pars reticulata rat

项目摘要

It has been known that the ventrolateral part of the striatum (CPvl) receives cortical fibers from the orofacial motor area and send projection fibers to the dorsolateral part of the substantia nigra pars reticulata (SNdl), which contains many neurons projecting to the premotor neuron pools for the orofacial motor nuclei. Recently, we found that the CPvl also received amygdaloid fibers from the rostral part of the basolateral nucleus (BLr). In the present study, we examined the possible pathway from the BLr to the SNdl via the CPvl by using the combined anterograde and retrograde tracing technique, and the anterograde tracing combined with immunohistochemistry for chemical markers of the striatonigral neurons. After ipsilateral injections of biotinylated dextran amine (BDA) into the BLr and cholera toxin B subunit (CTb) into the SNdl, a prominent overlapping distribution of BDA-labeled fibers and CTb-labeled neuronal cell bodies was found in the CPvl at the level of the decussation of the anterior commissure. In this region, electron microscopic observations revealed that the BDA-labeled axon terminals containing densely-packed, round synaptic vesicles made asymmetrical synaptic contacts mostly with the dendritic spines of striatal neurons, some of which were labeled with CTb. Furthermore, we demonstrated that the BDA-labeled BLr fibers formed synapses with the CPvl neurons showing immunoreactivity for preprotachykinin A and preprodynorphin, both of which are chemical makers for the striatonigral neurons. Thus, the BLr-CPvl-SNdl pathway revealed by the present study may be involved in the control of emotional/orofacial movements.

众所周知，纹状体的腹外侧部分（CPvl）接收来自口面运动区的皮质纤维，并将投射纤维发送到黑质网状部（SNdl）的背外侧部分，其中包含许多投射到口面运动核的运动前神经元池的神经元。最近，我们发现CPvl还接收来自基底外侧核（BLr）的喙部的杏仁纤维。在本研究中，我们利用顺行和逆行联合示踪技术，以及顺行示踪与免疫组织化学相结合的纹状体黑质神经元化学标记物，检查了从 BLr 经 CPvl 到 SNdl 的可能途径。同侧将生物素化葡聚糖胺 (BDA) 注射到 BLr 并将霍乱毒素 B 亚基 (CTb) 注射到 SNdl 后，在前连合交叉水平的 CPvl 中发现 BDA 标记的纤维和 CTb 标记的神经元细胞体的显着重叠分布。在该区域，电子显微镜观察显示，BDA 标记的轴突末端含有密集的圆形突触小泡，主要与纹状体神经元的树突棘形成不对称的突触接触，其中一些树突棘被 CTb 标记。此外，我们证明BDA标记的BLr纤维与CPvl神经元形成突触，显示出前速激肽A和前强啡肽的免疫反应性，这两者都是纹状体黑质神经元的化学标记物。因此，本研究揭示的BLr-CPvl-SNdl通路可能参与情绪/口面部运动的控制。