STUDY OF BLOOD AND BONE MARROW CELLS IN MINIATURE SWINE

为探讨小型猪作为新型实验动物的可行性，从饲养管理、采血技术、骨髓穿刺技术等方面对小型猪进行了研究。在繁殖技术、血液和骨髓取样方面没有问题。在此基础上，对小型猪的血液学指标进行了测定，并对血液和骨髓细胞进行了形态学分析，以了解小型猪的特点，为小型猪的医学研究提供依据。Howell-Jolly小体常被观察到。血小板计数为33000/ul，与猴相似。外周血和骨髓细胞形态基本与大鼠相似，但无核，与犬和猴相同。中性粒细胞中过氧化物酶活性较高，嗜酸性粒细胞中酯酶活性较低，从而研究了小型猪血液和骨髓细胞的形态学特征，为小型猪的研究提供了依据。

To investigate the usefulness of miniature swine for a new laboratory animals, management for breeding and techniques for blood sampling and bone marrow puncture were applied. There ware no problems in techniques for breeding, and blood and bone marrow sampling. Suitable site for blood sampling was jugular vein and 3rd sternal for bone marrow puncture.Based on these results, measurement for hematological parameters and morphological analysis of blood and bone marrow cells were conducted to find out the characteristics of this animal for medical research.Erythrocyte counts of miniature swine was higher than other large animals, and smaller in size. Howell-Jolly body was usually observed. Platelet count was 33000/ul which was resembling to that of monkeys. Morphology of peripheral blood and bone marrow cells were generally similar to those in rats without nuclear shape that was same as dogs and monkeys. High peroxidase activity in neutrophils and low esterase activity in eosinophils were also detected.Thus, morphological characteristics of blood and bone marrow cells in miniature swine were investigated and these data would be useful for research employing this animal.