Genome analysis in Methanosarcina mazei GÖ1: Mechanisms for the adaptation to different carbon- and energy sources

Methanosarcina mazei Gà1 的基因组分析：适应不同碳源和能源的机制

• 批准号: 5313622
• 负责人: Professor Dr. Uwe Deppenmeier
• 金额: --
• 依托单位: Institut für Mikrobiologie und Biotechnologie (IfMB)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5313622?language=en
• 关键词: Genome; analysis; Methanosarcina; mazei; Mechanisms

The mesophilic organism Methanosarcina mazei belongs to the kingdom Archaea and is able to convert H2 + CO2, methanol, methylamines and acetate to methane. Depending on the nature of the carbon- and energy source the organism uses the CO2-reducing, the methylotrophic, or the aceticlastic pathway of methanogenesis. Based on the genomic sequence we would like to analyse which genes are active in a specific pathway, how these genes are regulated and which regulatory networks may be involved. To understand the dynamic nature of gene function the global expression pattern in response to environmental changes will be elucidated. For this purpose genome wide expression profiling will be performed by the use of high-density DNA standard cDNA synthesis from mRNA preparations obtained from cells grown on different substrates or from cells harvested at different growth phases. In the second part of the project defined mutants of Ms. mazei will be created by gene replacement to verify the function of distinct genes. In addition, direct enzymatic assays using cell lysates of Ms. mazei shall contribute to an understanding of cellular processes that directly and indirectly affect methanogenesis.

中温微生物Methanosarcina mazei属于古细菌王国，能够将H2 + CO2，甲醇，甲胺和乙酸转化为甲烷。根据碳和能量来源的性质，生物体采用二氧化碳还原、甲基化或醋酸化途径的甲烷生成。基于基因组序列，我们想分析哪些基因在特定途径中是活跃的，这些基因是如何被调节的，哪些调节网络可能参与其中。为了了解基因功能的动态性质，将阐明响应环境变化的全球表达模式。为此，基因组宽表达谱将通过使用高密度DNA标准cDNA合成来完成，这些mRNA制备来自生长在不同底物上的细胞或不同生长阶段收获的细胞。在项目的第二部分，将通过基因替换来创建Ms. mazei的定义突变体，以验证不同基因的功能。此外，使用Ms. mazei的细胞裂解物进行的直接酶分析将有助于理解直接和间接影响甲烷生成的细胞过程。