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Comparative analysis of aceticlastic methanogenesis in Methanosarcina and Methanosaeta species

甲烷八叠球菌和甲烷八叠球菌乙酸产甲烷作用的比较分析

基本信息

批准号：
196331491
负责人：
Professor Dr. Uwe Deppenmeier
金额：
--
依托单位：
Institut für Mikrobiologie und Biotechnologie (IfMB)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/196331491?language=en
关键词：
Comparative analysis aceticlastic methanogenesis Methanosarcina

项目摘要

The major part of methane production on earth originates from the methyl group of acetate, but only the genera Methanosarcina (Ms.) and Methanosaeta (Mt.) are able to use acetate for methanogenesis and growth. Members of these genera are also of great importance for the productivity of biogas plants. The membrane-bound electron transport system and the energy conserving mechanism of Methanosaeta sp. are largely unknown. Even with the knowledge from the genome sequence of Mt. thermophila it cannot be explained how the utilization of acetate leads to a net synthesis of ATP. The overall goal of this project will be the comparison of acetate utilization in Ms. mazei and Mt. thermophila. The topics include the mechanism of acetate uptake and the function of the acetyl-CoA synthetases. Also of central importance is the pyrophosphatase from Mt. thermophila, which will be analysed with respect to possible regulatory effect of adenine nucleotides. In addition, we will analyze whether pyrophosphate, which is produced in the course of acetate activation, is used for energy conservation in Mt. thermophila. This organism only contains gene for the production of the F420H2 dehydrogenase and the heterodisulfide reductase. All other energy conserving oxidoreductases found in Methanosarcina species are obviously missing. It is also unclear how electrons from reduced ferredoxin are transferred into the respiratory chain for heterodisulfide reduction. To shed light on these issues we would like to analyze the electron transport chain of this organism in detail.

甲烷八叠球菌属（Methanosarcina（Ms.）和Methanosaeta（Mt.）能够利用乙酸盐进行产甲烷和生长。这些属的成员对沼气厂的生产力也非常重要。甲烷菌的膜结合电子传递系统和能量保存机制在很大程度上是未知的。即使有了来自Mt.因此，不能解释乙酸盐的利用如何导致ATP的净合成。本项目的总体目标是比较马泽女士和马泽山的醋酸盐利用率。嗜热菌主题包括乙酸吸收的机制和乙酰辅酶A合成酶的功能。同样重要的是来自Mt.嗜热菌，这将分析腺嘌呤核苷酸的可能的调节作用。此外，我们将分析在乙酸活化过程中产生的焦磷酸盐是否用于Mt.嗜热菌该微生物仅含有生产F420H2脱氢酶和异二硫还原酶的基因。在甲烷八叠球菌属物种中发现的所有其他节能氧化还原酶明显缺失。还不清楚还原铁氧还蛋白的电子如何转移到呼吸链中进行异二硫键还原。为了阐明这些问题，我们想详细分析这种生物体的电子传递链。