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Molecular analysis of rye-drived midget chromosomes by microdissection

Molecular analysis of rye-drived midget chromosomes by microdissection
通过显微切割对黑麦驱动的侏儒染色体进行分子分析
批准号:
13660007
负责人:
MURATA Minoru
金额:
$2.3万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002

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中文摘要
翻译
用紫外激光对(谷类)-中国春的小染色体进行显微解剖,并用DOP (degeneredoligonucleotide - primed)-PCR扩增DNA。荧光原位杂交和基因组Southern杂交清楚地表明,扩增的DNA来源于侏儒染色体。序列分析表明,扩增的DNA包含低拷贝序列、反转录转座子样序列和小卫星序列。从侏儒染色体中。其中一些与已知基因的同源性相对较高,但似乎没有一个与线粒体和/或叶绿体功能有关。
英文摘要
The midget chromosomes in (cereale)-Chinese Spring were microdissected by UV-laser, and the DNA was amplified by using DOP (Degenerate Oligonucleotide-Primed)-PCR. Fluorescence in situ and genomic Southern hybridizations clearly showed that the amplified DNA originated from the midget chromosome. Sequence analysis revealed that low -copy sequences as well as retrotransposon-like and minisatellite sequences were involved in the amplified DNA. In from the midget chromosomes. Some of them had relatively high homologies to those of known genes, but none seems to be related to the mitochondrial and/or chloroplastic functions.
期刊论文(6)
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会议论文
Cheng, Z.-J., Murata, M.: "A novel repetitive DNA sequences in the centromeric regions of A- and B-genome chromosomes in wheat"Ikusyugaku Kenkyu. 3(Suppl.2). 61 (2001)
Cheng, Z.-J., Murata, M.:“小麦 A 和 B 基因组染色体着丝粒区域的新型重复 DNA 序列”Ikusyugaku Kenkyu。
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通讯作者:
Ohdan, T., Murata, M.: "Cloning of DNA fragments from wheat chromosomes by microdissection"Gene and Genetic Systems. 76・6. 449 (2001)
Ohdan, T., Murata, M.:“通过显微切割从小麦染色体中克隆 DNA 片段”基因和遗传系统 76·6 (2001)。
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程治軍, 村田 稔: "コムギA, Bゲノム染色体のセントロメアに局在する新規反復配列"育種学雑誌 別冊. 3巻2号. 64 (2001)
Jigun Hodo,Minoru Murata:“位于小麦 A 和 B 基因组染色体着丝粒的新型重复序列”《育种杂志》,第 3 卷,第 2 期,64(2001 年)
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