Molecular analysis of rye-drived midget chromosomes by microdissection
Molecular analysis of rye-drived midget chromosomes by microdissection
批准号:
13660007
负责人:
MURATA Minoru
金额:
$2.3万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002
中文摘要
用紫外激光对(谷类)-中国春的小染色体进行显微解剖,并用DOP (degeneredoligonucleotide - primed)-PCR扩增DNA。荧光原位杂交和基因组Southern杂交清楚地表明,扩增的DNA来源于侏儒染色体。序列分析表明,扩增的DNA包含低拷贝序列、反转录转座子样序列和小卫星序列。从侏儒染色体中。其中一些与已知基因的同源性相对较高,但似乎没有一个与线粒体和/或叶绿体功能有关。
英文摘要
The midget chromosomes in (cereale)-Chinese Spring were microdissected by UV-laser, and the DNA was amplified by using DOP (Degenerate Oligonucleotide-Primed)-PCR. Fluorescence in situ and genomic Southern hybridizations clearly showed that the amplified DNA originated from the midget chromosome. Sequence analysis revealed that low -copy sequences as well as retrotransposon-like and minisatellite sequences were involved in the amplified DNA. In from the midget chromosomes. Some of them had relatively high homologies to those of known genes, but none seems to be related to the mitochondrial and/or chloroplastic functions.
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Cheng, Z.-J., Murata, M.: "A novel repetitive DNA sequences in the centromeric regions of A- and B-genome chromosomes in wheat"Ikusyugaku Kenkyu. 3(Suppl.2). 61 (2001)
Cheng, Z.-J., Murata, M.:“小麦 A 和 B 基因组染色体着丝粒区域的新型重复 DNA 序列”Ikusyugaku Kenkyu。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Ohdan, T., Murata, M.: "Cloning of DNA fragments from wheat chromosomes by microdissection"Gene and Genetic Systems. 76・6. 449 (2001)
Ohdan, T., Murata, M.:“通过显微切割从小麦染色体中克隆 DNA 片段”基因和遗传系统 76·6 (2001)。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
程治軍, 村田 稔: "コムギA, Bゲノム染色体のセントロメアに局在する新規反復配列"育種学雑誌 別冊. 3巻2号. 64 (2001)
Jigun Hodo,Minoru Murata:“位于小麦 A 和 B 基因组染色体着丝粒的新型重复序列”《育种杂志》,第 3 卷,第 2 期,64(2001 年)
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Ohdan, T., Murata, M.: "Cloning of DNA fragments from wheat chromosomes by microdissection"Gene and Genetic Systems. 76.6. 449 (2001)
Ohdan, T., Murata, M.:“通过显微切割从小麦染色体中克隆 DNA 片段”基因和遗传系统。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
共 6 条
Genome engineering and gene expression analysis in a model plant Arabidopsis thaliana
-
批准号:22310129
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.48万
-
财政年份:2010
-
负责人:MURATA Minoru
-
依托单位:
Study on the structure of nonnegative solutions for parabolic equations and the perturbation theory of elliptic operators
-
批准号:21540164
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.58万
-
财政年份:2009
-
负责人:MURATA Minoru
-
依托单位:
Asymptotic analysis of heat kernels and Green functions and its applications
-
批准号:17340034
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$4.42万
-
财政年份:2005
-
负责人:MURATA Minoru
-
依托单位:
A synthetic study of positive solutions to elliptic and parabolic partial differential equations
-
批准号:13440042
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$4.67万
-
财政年份:2001
-
负责人:MURATA Minoru
-
依托单位:
A versatile study of positive solutions to parabolic and elliptic partial differential equations
-
批准号:11440039
-
项目类别:Grant-in-Aid for Scientific Research (B).
-
资助金额:$1.6万
-
财政年份:1999
-
负责人:MURATA Minoru
-
依托单位:
Construction of chromosome -specific DNA libraries by laser-microdissection-Molecular analysis of homoeologous group 1 chromosomes in wheat.
-
批准号:11660005
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.37万
-
财政年份:1999
-
负责人:MURATA Minoru
-
依托单位:
Structure of positive solutions to second order elliptic and parabolic partial differential equations
-
批准号:09440049
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$3.65万
-
财政年份:1997
-
负责人:MURATA Minoru
-
依托单位:
MOLECULAR STUDIES ON NUCLEAR GENE (S) INTERACTING RYE CYTOPLASM ORDERING OF YAC CLONES TO IDENTIFY THE GENE (S)
-
批准号:06660007
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1994
-
负责人:MURATA Minoru
-
依托单位:
MOLECULAR STUDIES ON NUCLEAR GENE(S) INTERACTING WITH RYE CYTOPLASM Construction of YAC library with telomere sequences
-
批准号:04660006
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.41万
-
财政年份:1992
-
负责人:MURATA Minoru
-
依托单位:
Molecular Studies on Nuclear Gene(s) Interacting with Rye Cytoplasm
-
批准号:02660007
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.09万
-
财政年份:1990
-
负责人:MURATA Minoru
-
依托单位:
海外基金