Genome engineering and gene expression analysis in a model plant Arabidopsis thaliana

为了重建拟南芥的核基因组，我们将起源于噬菌体P1的loxP序列引入到染色体中，并创建了30个每条染色体携带一到两个拷贝的loxP的品系。这些loxP位点通过杂交结合到基因组中，并用Cre重组酶诱导序列特异性重组。PCR和FISH分析表明，相互易位可能是由位于不同染色体上的两个loxP位点重组引起的。也有人认为，与相互易位相关的染色质结构的改变，导致了表型的变化。

In order to reconstruct the nuclear genome of Arabidopsis thaliana, we introduced the LoxP sequence, which originated from bacteriophase P1, into the chromosomes, and have created 30 lines carrying one or two copies of LoxP per chromosome. These LoxP sites were combined in the genome by crossing, and the sequence-specific recombination was induced with the Cre-recombinase. PCR and FISH analyses revealed that reciprocal translocations are possibly induced by recombination between two LoxP sites located on different chromosomes. It was also suggested that alteration of chromatin structure associated with the reciprocal translocation, induces phenotypic changes.