Genome engineering and gene expression analysis in a model plant Arabidopsis thaliana

为了重建拟南芥的核基因组，我们将起源于细菌期P1的LoxP序列引入到染色体中，并创建了30个染色体携带一个或两个LoxP拷贝的品系。这些LoxP位点通过交叉组合在基因组中，用Cre-recombinase诱导序列特异性重组。PCR和FISH分析显示，相互易位可能是由位于不同染色体上的两个LoxP位点之间的重组引起的。也有人认为，染色质结构的改变与相互易位相关，诱导表型变化。

In order to reconstruct the nuclear genome of Arabidopsis thaliana, we introduced the LoxP sequence, which originated from bacteriophase P1, into the chromosomes, and have created 30 lines carrying one or two copies of LoxP per chromosome. These LoxP sites were combined in the genome by crossing, and the sequence-specific recombination was induced with the Cre-recombinase. PCR and FISH analyses revealed that reciprocal translocations are possibly induced by recombination between two LoxP sites located on different chromosomes. It was also suggested that alteration of chromatin structure associated with the reciprocal translocation, induces phenotypic changes.