Identification of glioma specific genes for the molecular marker

分子标记的神经胶质瘤特异性基因的鉴定

• 批准号: 13671465
• 负责人: TODA Masahiro
• 金额: $ 2.18万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671465/
• 关键词: glioma; gene chip; SAGE; cancer; diagnosis; RT-PCR; EST; 脳; 網羅的解析; 中枢神経系

Malignant gliomas are proliferative and invasive with poor prognosis. Although the recent progress of therapeutics has been shown in radiotherapy, chemotherapy, and gene therapy, the prognosis of glioma has not be improved. In order to identify specific molecules that can be targets for therapy and diagnostic markers of glioma, we used Affymetrix DNA chip technology and NCBI (National Center for Biotechnology Information) database including EST (expressed sequence tags) and SAGE (serial analysis of gene expression). First, we analyzed the gene expression in 11 glioma tissues compared with the average of that in 3 normal brain tissues using DNA chip containing 11000 Unigene clones. Sixteen genes were found to be expressed more than 3 times in all glioma tissues analyzed than normal brain. We then analyzed the expression of these selected genes by EST and SAGE programs in NCBI database. Among the 16 genes, five genes including the known glioma antigens, WAF1 and Tenascin C, showed to be overexpressed in cancer. By RT-PCR analysis of these 5 selected genes, we found a gene which shows a significantly higher expression in gliomas compared with normal brains. Using quantitative RT-PCR system with specific primers for this gene, we plan to establish a quick diagnosis method of glioma in the clinic

恶性胶质瘤具有增殖性和侵袭性，预后差。尽管在放射治疗、化疗和基因治疗方面取得了新的进展，但脑胶质瘤的预后并未得到改善。为了找出可以作为胶质瘤治疗和诊断标记物的特定分子，我们使用了Affymetrix DNA芯片技术和NCBI(国家生物技术信息中心)数据库，包括EST(表达序列标签)和SAGE(基因表达序列分析)。首先，我们利用包含11000个Unigene克隆的基因芯片分析了11例脑胶质瘤组织和3例正常脑组织的基因表达。在所有分析的胶质瘤组织中，有16个基因的表达高于正常脑组织3倍以上。然后利用NCBI数据库中的EST和SAGE程序对所选基因的表达进行分析。在这16个基因中，包括已知的胶质瘤抗原WAF1和Tenascin C在内的5个基因在癌症中过度表达。通过对这5个基因的RT-PCR分析，我们发现了一个在胶质瘤中表达明显高于正常脑组织的基因。我们计划利用针对该基因的特异性引物，建立一种临床快速诊断脑胶质瘤的方法。

项目成果

Toda M, Iizuka Y, Kawase T, Uyemura K, Kawakami Y: "Immuno-viral therapy of brain tumors by combination of viral therapy with cancer vaccination using a replication-conditional HSV"Cancer Gene Ther. 9. 356-364 (2002)

Toda M、Iizuka Y、Kawase T、Uyemura K、Kawakami Y：“使用复制条件 HSV 将病毒疗法与癌症疫苗接种相结合，对脑肿瘤进行免疫病毒疗法”Cancer Gene Ther。

戸田正博, 戸山芳昭: "樹状細胞"臨床整形外科. 137・6. 732-733 (2002)

户田正宏、富山义明：《树突状细胞》《临床骨科》137・6（2002）。

Mikimi Y, Toda M, Watanabe M, Nakamura M, Toyama Y, Kawakami Y: "A simple and reliable behavioral analysis of locomotor function after spinal cord injury in mice"J Neurosurg. 97. 142-147 (2002)

Mikimi Y、Toda M、Watanabe M、Nakamura M、Toyama Y、Kawakami Y：“小鼠脊髓损伤后运动功能的简单可靠的行为分析”J Neurosurg。

Toda,M, Iizuka,Y, Yu,W, Imai,T, Ikeda,E, Yoshida,K, Kawase,T, Kawakami,Y, Okano,H, Uyemura,K: "Expression of the neural RNA-binding protein Musashil in human gliomas"Glia. 34. 1-7 (2001)

Toda,M, Iizuka,Y, Yu,W, Imai,T, Ikeda,E, Yoshida,K, Kawase,T, Kawakami,Y, Okano,H, Uyemura,K：“神经 RNA 结合蛋白 Musashil 的表达

Toda M, et al.: "Expression of the neural RNA-binding protein musashil in human gliomas"Glia. 34. 1-7 (2001)

Toda M 等人：“神经 RNA 结合蛋白 musashil 在人类神经胶质瘤中的表达”Glia。