PKC-zeta-dependent Sp1 phosphorylation: Identification of phosphorylated amino acids, demonstration of functional significance, generation and use of novel phospho-specific Sp1 antibodies

PKC-zeta 依赖性 Sp1 磷酸化：磷酸化氨基酸的鉴定、功能意义的论证、新型磷酸化特异性 Sp1 抗体的生成和使用

• 批准号: DP0345071
• 负责人: Prof Levon Khachigian
• 金额: $ 14.64万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-02-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0345071
• 关键词: PKC; zeta; dependent; Sp1; phosphorylation

Sp1 is a widely expressed transcription factor that controls the basal expression of virtually every mammalian gene, including that of PDGF-B. We recently reported that PDGF-B expression atypical protein kinase C-zeta phosphorylation of Sp1. Building on these seminal findings, this project will first, delineate the specific amino acid residues in the zinc finger region of Sp1 phosphorylated by PKC-zeta; second, demonstrate the functional importance of these site-specific modifications in the PKC-zeta-Sp1-PDGF-B system and the expression of other genes, and third, generate and use novel antibodies uniquely recognising phosphorylated Sp1 as molecular and diagnostic agents.

Sp1是一种广泛表达的转录因子，控制几乎所有哺乳动物基因的基础表达，包括PDGF-B。 我们最近报道，PDGF-B表达的非典型蛋白激酶C-zeta磷酸化的Sp1。基于这些开创性的发现，该项目将首先描述由PKC-zeta磷酸化的Sp1锌指区域中的特定氨基酸残基;其次，证明这些位点特异性修饰在PKC-zeta-Sp1-PDGF-B系统和其他基因表达中的功能重要性;第三，产生并使用独特识别磷酸化Sp1的新型抗体作为分子和诊断剂。