Resolution of the wound healing mechanism of dertin-pulp complex and application of the knowledge on it to regenerative medicine

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In this subject, aiming at development of dentin regeneration therapy and its technology, various experiments were conducted, and the following results were obtained.In vitro experiments, it became clear that alginic acid gel is useful as scaffold for dentin regeneration, that neurotrophic factor affects differentiation and mineralization of a pulp cells, that tenascin inhibits cell adhesion in pulp cells and cell adhesion is adjusted through the change of tenascin expression regulated by the growth factor, that expression of DSPP mRNA becomes strong and the amount of matrix increases when BMP-2 is added onto the pulp cells, and that PDGF participates in dentin formation in the wound healing process of pulp tissue, etc. On the other hand, in vivo experiments, it became clear that phosphophorynlcollagen complex serves as effective carrier of BMP-2, that the combined use of CCl2 laser and adhesive composite resin in direct pulp capping is effective, that pulp tissue contains the substance and cell which have hard tissue formation activity, that calcium phosphate cement are excellent in biocompatibility and it can be applied as pulp capping material, that rFIP-2B mRNA expression increases in the wound healing process in pulp cells and the promoter of this gene is activated by wound stimulus, that pulp stem cell introducing method is effective in dentin regeneration, that EMD promotes dentin regeneration when the environment of wound part of pulp tissue is optimum, that metallothionein participates in proliferation and differentiation of pulp cells in wound healing of pulp tissue after cavity preparation, and that cell death control is very important in wound healing process of pulp tissue, etc.Further experiments for developing technology which guides effective regeneration of dentin is to be continued after this research subject is over.