The effect of food factors on postprandial hyperlipidemia - the search for food factors and the clarification of those mechanisms -

近年来，餐后高脂血症已被认为是动脉粥样硬化发展的危险因素。四年前，我们评估了三种不同脂肪含量的蛋黄酱对高脂血症的影响。结果表明，摄入甘油三酯半能蛋黄酱后，小鼠血清和乳清中甘油三酯水平明显低于摄入甘油三酯半能蛋黄酱的小鼠。此外，摄入DG蛋黄酱后的乳杆菌甘油三酯水平高于摄入TG蛋黄酱后的水平。此外，我们还发现，脂肪负荷后的乳酸甘油三酯水平随着绿茶摄入量的增加而降低(p=0.0093)。第二年，除了绿茶，我们还评估了柠檬对餐后高脂血症的影响。结果表明，与单独摄入脂肪相比，摄入含有柠檬的脂肪显著降低了血浆乳糜蛋白TG水平的升高。此外，血浆甘油三酯水平的形成也被推迟。此外，柠檬类黄酮和柠檬fl…更多的乌头翁糖苷元显示出类似的结果。因此，这些发现表明柠檬类黄酮苷元抑制脂肪吸收。第三年，我们利用跨孔培养的人结肠癌细胞系Caco-2的单层细胞，研究了EGC和EGCG对棕榈酸在肠道上皮运输的影响。结果表明，EGCG对棕榈酸的吸收有明显的抑制作用，而EGC对其无明显影响。去年，我们测定了肠淋巴流量、淋巴甘油三酯和胆固醇水平，以证实柠檬对肠道脂肪吸收的抑制作用。结果发现，与对照组相比，柠檬黄酮苷元粉组的淋巴流率下降幅度更大。结论：富含绿茶和柠檬的黄酮类化合物抑制肠道脂肪吸收，可能有助于预防餐后高脂血症。较少

Recently, postprandial hyperlipidemia has been recognized to be a risk factor for atherosclerosis development. Four years ago, we evaluated the effect of three kinds of mayonnaise with differing fat contents on hyperlipidemia. The results showed that the serum and chylomicron TG levels after the ingestion of TG half-energy mayonnaise were significantly lower than those for the TG mayonnaise. In addition, the chylomicron TG levels after ingestion of the DG mayonnaise were higher than those for the TG mayonnaise. Moreover, we showed that the chylomicron TG levels after the fat load decreased with the intake of green tea (p=0.0093).The next year, in addition to green tea, we evaluated the effect of lemon on postprandial hyperlipidemia. The results showed that the intake of fat with lemon significantly reduced the rise of plasma chylomicron TG levels in comparison to the intake of fat alone. Moreover, the formation of plasma TG levels was delayed. In addition, lemon flavonoids and lemon fl … More avonoids aglycon showed similar results. Therefore, these findings suggested that lemon flavonoid aglycon inhibited fat absorption.The third year, we examined the effect of EGC and EGCg on the intestinal epithelial transport of palmitic acid, using a monolayer of the human colonic cell line Caco-2 grown in transwells, which is a common model of human intestinal absorption. The results showed that the absorption of palmitic acid was significantly inhibited by EGCg but not EGC.Last year, we measured the intestinal lymph flow and lymph TG and cholesterol levels in order to confirm how lemon inhibited intestinal fat absorption. As a result, we found that the lymph flow rate decreased to a greater extent in the lemon flavonoid aglycon powder group in comparison to the control group. In addition, the absorption volume of TG significantly decreased in the lemon flavonoid aglycon powder group.In conclusion, our results have shown that flavonoids rich in green tea and lemon inhibit intestinal fat absorption, which may contribute to the prevention of postprandial hyperlipidemia. Less