The effect of food factors on postprandial hyperlipidemia - the search for food factors and the clarification of those mechanisms -

最近，餐后高脂血症被认为是动脉粥样硬化发展的一个危险因素。四年前，我们评估了三种不同脂肪含量的蛋黄酱对高脂血症的影响。结果表明：TG半能蛋黄酱饲喂后血清和乳糜微粒TG水平显著低于TG半能蛋黄酱饲喂后血清和乳糜微粒TG水平；此外，食用DG蛋黄酱后乳糜微粒TG水平高于TG蛋黄酱。此外，我们发现脂肪负荷后乳糜微粒TG水平随着摄入绿茶而降低（p=0.0093）。第二年，除了绿茶，我们还评估了柠檬对餐后高脂血症的影响。结果表明，与单独摄入脂肪相比，柠檬脂肪摄入显著降低了血浆乳糜微粒TG水平的升高。此外，血浆TG水平的形成被推迟。此外，柠檬黄酮类化合物和柠檬脂多糖也有相似的结果。因此，这些结果表明柠檬类黄酮多糖抑制脂肪吸收。第三年，我们研究了EGC和EGCg对棕榈酸肠上皮运输的影响，使用了生长在transwells中的单层人结肠细胞系Caco-2，这是一种常见的人类肠道吸收模型。结果表明，EGCg对棕榈酸的吸收有明显的抑制作用，而EGC对棕榈酸的吸收没有明显的抑制作用。去年，我们测量了肠道淋巴流量、淋巴TG和胆固醇水平，以证实柠檬如何抑制肠道脂肪吸收。结果，我们发现柠檬类黄酮多糖粉组的淋巴流速比对照组下降得更大。此外，柠檬类黄酮多糖粉组TG的吸收量显著降低。综上所述，我们的研究结果表明，绿茶和柠檬中富含的黄酮类化合物可以抑制肠道脂肪的吸收，这可能有助于预防餐后高脂血症。少

Recently, postprandial hyperlipidemia has been recognized to be a risk factor for atherosclerosis development. Four years ago, we evaluated the effect of three kinds of mayonnaise with differing fat contents on hyperlipidemia. The results showed that the serum and chylomicron TG levels after the ingestion of TG half-energy mayonnaise were significantly lower than those for the TG mayonnaise. In addition, the chylomicron TG levels after ingestion of the DG mayonnaise were higher than those for the TG mayonnaise. Moreover, we showed that the chylomicron TG levels after the fat load decreased with the intake of green tea (p=0.0093).The next year, in addition to green tea, we evaluated the effect of lemon on postprandial hyperlipidemia. The results showed that the intake of fat with lemon significantly reduced the rise of plasma chylomicron TG levels in comparison to the intake of fat alone. Moreover, the formation of plasma TG levels was delayed. In addition, lemon flavonoids and lemon fl … More avonoids aglycon showed similar results. Therefore, these findings suggested that lemon flavonoid aglycon inhibited fat absorption.The third year, we examined the effect of EGC and EGCg on the intestinal epithelial transport of palmitic acid, using a monolayer of the human colonic cell line Caco-2 grown in transwells, which is a common model of human intestinal absorption. The results showed that the absorption of palmitic acid was significantly inhibited by EGCg but not EGC.Last year, we measured the intestinal lymph flow and lymph TG and cholesterol levels in order to confirm how lemon inhibited intestinal fat absorption. As a result, we found that the lymph flow rate decreased to a greater extent in the lemon flavonoid aglycon powder group in comparison to the control group. In addition, the absorption volume of TG significantly decreased in the lemon flavonoid aglycon powder group.In conclusion, our results have shown that flavonoids rich in green tea and lemon inhibit intestinal fat absorption, which may contribute to the prevention of postprandial hyperlipidemia. Less