Investigation of effects of pest controlling methods including alternative fumigants of methyl bromide on DNA of museum objects.

调查害虫控制方法（包括甲基溴替代熏蒸剂）对博物馆物品 DNA 的影响。

• 批准号: 15300297
• 负责人: KIGAWA Rika
• 金额: $ 7.87万
• 依托单位: Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300297/
• 关键词: Cultural properties; DNA analysis; Fumigants; Biodeterioration

Treatments for pest eradication on museum objects are often indispensable. But such treatments sometimes cause adverse effects on museum objects. When we look at natural specimens, DNA analysis for taxonomic studies have become increasing common. But some conservators had become concerned about the possibility that some fumigants may adversely affect DNA molecules of natural specimens. Thus we investigated the effects of fumigants which have been used for museum collections on DNA of especially natural specimens in this research. The effects of several treatments for pest eradication on natural specimen DNA were examined using freeze-dried mushroom and freeze-dried muscle. The data clearly showed that the fumigants methyl bromide, methyl bromide/ ethylene oxide mixed gas, ethylene oxide, propylene oxide, and methyl iodide all caused significant degradation of specimen DNA, even with a single fumigation. Subsequent steps at amplification of certain DNA fragments by PCR (polymerase chain reaction) were difficult in these samples. There were more problems in amplifying larger DNA fragments than smaller fragments. The main cause of the decreased efficiency of PCR was thought to be the degradation of template DNA. On the other hand, thermal methods (heating and low temperature), 60 percent carbon dioxide treatment, and fumigation by sulfuryl fluoride seemed scarcely to affect the DNA molecules of the specimens, allowing subsequent PCR to be successfully performed. Direct sequencing of the PCR products was also performed to check the possibility of modifications to the DNA sequences. When the PCR products were amplified to the sufficient amount for the direct sequencing, the DNA sequences were normal in our examination of the few gene regions.

博物馆展品的虫害根除处理往往是必不可少的。但这样的处理有时会对博物馆的物品产生不利影响。当我们观察自然标本时，用于分类学研究的DNA分析变得越来越普遍。但是一些保护人员开始担心一些熏蒸剂可能会对自然标本的DNA分子产生不利影响。因此，我们调查的熏蒸剂已被用于博物馆收藏品的DNA，特别是自然标本在这项研究中的影响。以冻干蘑菇和冻干肌肉为材料，研究了几种杀虫处理对天然样品DNA的影响。数据清楚地表明，熏蒸剂甲基溴，甲基溴/环氧乙烷混合气体，环氧乙烷，环氧丙烷，碘甲烷都造成了显着的降解标本DNA，即使是一个单一的熏蒸。在这些样品中，通过PCR（聚合酶链反应）扩增某些DNA片段的后续步骤是困难的。扩增较大的DNA片段比扩增较小的DNA片段存在更多的问题。PCR效率下降的主要原因是模板DNA的降解。另一方面，热方法（加热和低温），60%的二氧化碳处理和硫酰氟熏蒸似乎几乎不影响样本的DNA分子，使随后的PCR成功地进行。还进行PCR产物的直接测序以检查DNA序列修饰的可能性。当PCR产物扩增到足以直接测序的量时，在我们对少数基因区域的检查中，DNA序列是正常的。

项目成果

燻蒸剤等各種殺虫処理が資料DNAに及ぼす影響

熏蒸剂等各种杀虫剂处理对物质 DNA 的影响

• 发表时间: 2003
• 期刊: 文化財保存修復学会第25回大会研究発表要旨集 25
• 作者: 木川りか;三浦定俊;後出秀聡;木村広
• 通讯作者: 木村広

Effects of Pest Eradication Such as Various Fumigants on DNA of Natural Specimens

各种熏蒸剂等灭害剂对天然标本DNA的影响

• 发表时间: 2003
• 期刊: Bunkazaihozonshufuku gakkai, 25^<th> annual meeting, abstract of papers 25
• 作者: Rika Kigawa;Hideaki Nochide;Hiroshi Kimura;Sadatoshi Miura
• 通讯作者: Sadatoshi Miura

燻蒸剤等各種殺虫処理が標本のタンパク質に及ぼす影響

熏蒸剂等各种杀虫处理对标本蛋白质的影响

• 发表时间: 2005
• 期刊: 日本文化財科学会第22回大会研究発表要旨集 22
• 作者: 木川りか;Tom Strang;Gregory Young
• 通讯作者: Gregory Young

Kigawa, R., Nochide, H., Kimura, H., Miura, S.: "Effects of various fumigants, thermal methods and carbon dioxide treatment on DNA extraction and amplification : A case study on freeze-dried mushroom and freeze-dried muscle specimens"Collection Forum. 18(

Kikawa, R.、Nochide, H.、Kimura, H.、Miura, S.：“各种熏蒸剂、热方法和二氧化碳处理对 DNA 提取和扩增的影响：冻干蘑菇和冻干蘑菇的案例研究

燻蒸剤等各種殺虫殺菌処理がタンパク質材質(標本、膠、絹)に及ぼす影響の検討

检查熏蒸剂等各种杀虫和灭菌处理对蛋白质材料（标本、胶水、丝绸）的影响

• 发表时间: 2006
• 期刊: 文化財保存修復学会第28回大会研究発表要旨集 28
• 作者: 木川りか;早川典子;Tom Strang;Gregory Young;木村広
• 通讯作者: 木村広