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Identification of Host Factors for Virus Particle Release Based on Yeast Genetics

Identification of Host Factors for Virus Particle Release Based on Yeast Genetics
基于酵母遗传学的病毒颗粒释放宿主因素的鉴定
批准号:
15390152
负责人:
MORIKAWA Yuko
金额:
$9.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2005

项目摘要

项目成果

MORIKAWA Yuko的其他基金

相关文献

中文摘要
翻译
我们先前已经表明酿酒酵母支持人类免疫缺陷病毒1型(HIV-1)Gag颗粒组装,表明酵母具有HIV-1 Gag颗粒所需的所有宿主因子,为了鉴定负责HIV-1 Gag转运和随后的颗粒出芽的宿主因子/机制,1)使用最近研究提出的一系列内体途径的酵母遗传突变体,我们检查了Gag运输途径; 2)确定了HIV-2Gag对酵母中颗粒组装的负面影响;和3)通过使用CytoTrap双杂交测定,试图鉴定负责Gag细胞内运输的新宿主因子。1)Gag运输的内体途径:使用一系列酵母内体遗传突变体,我们发现早期和晚期内体t-SNARE的缺陷显示出颗粒产生的严重减少,但令人惊讶的是,E类液泡蛋白分选分子在很大程度上被破坏。定义Ga中t-SNARE的角色 关于我们 g贩运,我们在人细胞中耗尽/过度表达它们的直系同源物。结果表明,t-SNARE是HIV-1 Gag转运的积极因素。2)HIV-2 Gag对酵母中颗粒组装的负面影响:我们还扩展了我们先前使用不同灵长类慢病毒Gag的研究,并表明酵母不支持HIV-2或猿猴免疫缺陷病毒(SIV)mac Gag颗粒的产生。研究表明,i)HIV-2 MA的N-末端一半的存在导致酵母中颗粒出芽失败; ii)Gag被转运至质膜; iii)在不存在盐的情况下,Gag容易从膜上解离; iv)因此,颗粒出芽被膜皱褶结构阻止。总之,这些数据表明酵母可能缺乏HIV-2和SIVmac Gag VLP出芽所必需的因子。3)负责Gag细胞内转运的新宿主因子的分离:我们使用酵母CytoTrap双杂交测定(其中蛋白质-蛋白质相互作用发生在质膜下的系统)并筛选人cDNA的表达文库(目前2-3个阳性)。少
英文摘要
We have previously shown that Saccharomyces cerevisiae supports human immunodeficiency virus type 1 (HIV-1) Gag particle assembly, indicating that yeast has all the host factors necessary for HIV-1 Gag particle, To identify the host factors/machinery responsible for HIV-1 Gag transport and subsequent particle budding, 1)using a series of yeast genetic mutants of endosomal pathways suggested by recent studies, we examined Gag trafficking pathways ; 2)defined the negative effect of HIV-2 Gag on particle assembly in yeast ; and 3)by use of CytoTrap two-hybrid assay, attempted to identify a novel host factor(s) responsible for intracellular transport of Gag.1)Endosomal pathways for Gag trafficking : Using a series of yeast genetic mutants of endosomal, we found that the defect of early and late endosomal t-SNAREs showed severe reduction in particle production but surprisingly, the class E vacuolar protein sorting molecules were largely dispensable. To define the roles of the t-SNAREs in Ga … More g trafficking, we depleted/over-expressed their orthologues in human cells. Results indicated that the t-SNAREs are positive factors for HIV-1 Gag transport.2)Negative effect of HIV-2 Gag on particle assembly in yeast : We also expanded our previous study using diverse primate lentiviral Gags and showed that yeast did not support production of HIV-2 or simian immunodeficiency virus (SIV) mac Gag particles. Studies revealed that, i)the presence of the N-terminal half of HIV-2 MA resulted in a failure of particle budding in yeast ; ii)the Gag was transported to the plasma membrane ; iii)the Gag was easily dissociated from the membrane in the absence of salt ; iv)as a result, particle budding was arrested with membrane ruffling structures. Together, these data suggest that yeast may lack a factor(s) necessary for HIV-2 and SIVmac Gag VLP budding.3)Isolation of a novel host factor(s) responsible for intracellular transport of Gag : We used yeast CytoTrap two-hybrid assay (a system in which protein-protein interactions occur underneath the plasma membrane) and screened an expression library of human cDNAs (2-3 positives at present). Less
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会议论文
Human immunodeficiency virus type 1 Gag assembly through assembly intermediates
人类免疫缺陷病毒 1 型 Gag 通过组装中间体进行组装
DOI: --
发表时间: 2004
期刊: J.Biol.Chem. 279
影响因子: --
作者: [Y.Morikawa, T.Goto, F.Momose]
通讯作者: F.Momose
DOI: --
发表时间:
期刊: Microbes and Infection (in press)
影响因子: --
作者: [S.Sakuragi, J.Sakuragi, Y.Morikawa, T.Shioda]
通讯作者: T.Shioda
Binding site for fungal b-lactose hymeglusin on cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase
胞质 3-羟基-3-甲基戊二酰辅酶 A 合酶上真菌 b-乳糖水凝素的结合位点
DOI: --
发表时间: 2004
期刊: Biochim.Biophys.Acta 1636
影响因子: --
作者: [H.Tomoda, N.Ohbayashi, Y.Morikawa, H.Kumagai, S.Omura.]
通讯作者: S.Omura.
The determinants of health literacy and social capital among workers and those effects on health
  • 批准号:
    18K10093
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.83万
  • 财政年份:
    2018
  • 负责人:
    MORIKAWA Yuko
  • 依托单位:
Molecular modification for MHC class I and II antigen presentation
  • 批准号:
    24659211
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
  • 财政年份:
    2012
  • 负责人:
    MORIKAWA Yuko
  • 依托单位:
Live-cell imaging of HIV replication component trafficking and assembly
  • 批准号:
    22390091
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.98万
  • 财政年份:
    2010
  • 负责人:
    MORIKAWA Yuko
  • 依托单位:
A study on the relation of shift work on hormonal change and diseases of prostate
  • 批准号:
    22590557
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.75万
  • 财政年份:
    2010
  • 负责人:
    MORIKAWA Yuko
  • 依托单位: