Effects of Night Work on the Immune Function

夜间工作对免疫功能的影响

• 批准号: 14570362
• 负责人: MORIKAWA Yuko
• 金额: $ 2.05万
• 依托单位: KANAZAWA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570362/
• 关键词: night work; shift work; immune function; Natural killer cell activity; lymphocyte surface antigen; circadian rhythm; nurse; stress; 自然免疫能; リンパ球分画; 看護師; 看護

To investigate the effects of shift work on immune parameters, such as lymphocyte subpopulations and natural killer cell activity, 105 nurses (28 males and 77 females) were studied. Blood samples of shift workers were collected from shift workers on two consecutive mornings. The first day's sampling was carried out in the morning just before the daytime shift, and sampling of the second day was carried out at the end of the night shift. For fixed daytime workers, blood samples were taken twice in the morning.(1)No significant differences were observed between shift workers and fixed day workers in the proportion of circulating lymphocyte subsets and NK cell activity from samples taken in the morning of the daytime work.(2)Shift workers exhibited higher level of the proportion of CD3 and CD4 in the morning after night time shift than daytime shift. NK cell activity and proportion of NK cells in the morning after night shift were lower than those in the morning of daytime shift. Female shift workers exhibited greater changes in CD4, 0D8, and CD16+56+ than male shift workers. Smokers showed greater changes in CD4 and CD 16+56+ than non-smokers. Fatigue feeling after night work related with an increase in the proportion of CD4 but with other immune parameters.(3)The difference of shift schedule before night shift did not show any difference in lymphocyte subsets and fatigue level.(4)The changes of immune function between daytime work and night work were larger in women than men. Smoking and fatigue related to immune function.These results suggest that night work has short term effects on diurnal rhythm of immune function. Even if these changes of immune function are limited to acute phase, repetition of modification of ordinal circadian rhythm of immune function might have long-term effects.

为了调查轮班工作对淋巴细胞亚群和自然杀伤细胞活性等免疫参数的影响，对 105 名护士（28 名男性和 77 名女性）进行了研究。连续两个早上从轮班工人身上采集血样。第一天的采样在白班前的早晨进行，第二天的采样在夜班结束时进行。对于固定白班工人，上午采血两次。（1）轮班工人与固定白班工人在白班上午采集的血液中循环淋巴细胞亚群比例和NK细胞活性无显着差异。（2）倒班工人夜班后早上的CD3和CD4比例高于白班。夜班后上午的NK细胞活性和NK细胞比例均低于白班上午。女性轮班工人比男性轮班工人表现出更大的 CD4、0D8 和 CD16+56+ 变化。吸烟者的 CD4 和 CD 16+56+ 变化比不吸烟者更大。夜班后的疲劳感与CD4比例增加有关，而与其他免疫参数有关。(3)夜班前的轮班时间差异，淋巴细胞亚群和疲劳程度没有差异。(4)白天工作和夜间工作之间免疫功能的变化，女性大于男性。吸烟和疲劳与免疫功能有关。这些结果表明夜间工作对免疫功能的昼夜节律有短期影响。即使免疫功能的这些变化仅限于急性期，重复改变免疫功能的昼夜节律可能会产生长期影响。