"Analysis of oxygen releasing behavior of hemoglobin-vesicles (nano-particles) and the mechanism of oxygenation of ischemic tissue."

“分析血红蛋白囊泡（纳米颗粒）的释氧行为和缺血组织的氧合机制。”

• 批准号: 16300162
• 负责人: SAKAI Hiromi
• 金额: $ 9.54万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300162/
• 关键词: Artificial Red Cells; Microcirculation; Hemoglobin; Ischemia; Oxygen release; Hemorheology; Artificial Blood; Liposome; レオロジー制御; 輸血代替

The purpose of this project was to clarify the mechanism and optimization of oxygenation of ischemic tissues by a suspension of hemoglobin-vesicles (HbV). One advantage of HbV is that O_2 affinity (P_<50>) of Hb can be easily regulated by manipulating the allosteric effectors coencapsulated in HbV. One concept is that the low P_<50> HbV would retain O_2 in the normal tissue while unloading O_2 to a targeted hypoxic tissue. HbV (P_<50> =9 mmHg) enhanced oxygenation of an ischemic part of hamster dorsal skin flap where tissue O_2 tension was significantly reduced. Injection of HbV increased the plasma viscosity, thus enhance the vasorelaxation and the resulting recovery of blood flow and oxygen delivery via collateral artery. In another experiment, low P_<50> HbV showed slower O_2 unloading in the occluded arterioles in comparison with normal P_<50> HbV. The contribution of the low P_<50> HbV as the source of O_2 supply exceeds that of RBC even with a small dosage of HbV, indicating the possible utilization of HbV for O_2 therapeutics. These findings indicates that manipulation of the physicochemical properties of HbV enables possible use of tailor-made artificial oxygen carriers for many clinical indications.

该项目的目的是阐明通过血红蛋白帮助的（HBV）的悬浮液来阐明缺血组织的氧合的机理和优化。 HBV的一个优点是，HB的O_2亲和力（P_ <50>）可以通过操纵HBV中的变构效应子来轻松调节。一个概念是，低P_ <50> HBV将在正常组织中保留O_2，同时将O_2卸载到靶向的低氧组织中。 HBV（P_ <50> = 9 mmHg）增强了仓鼠背皮肤皮瓣的缺血部分，其中组织O_2张力显着降低。注射HBV增加了血浆粘度，从而增强了血管延缓，并通过侧支动脉恢复了血流和氧气的递送。在另一个实验中，与正常的P_ <50> HBV相比，低p_ <50> HBV显示在阻塞动脉中卸载较慢的O_2。低p_ <50> HBV作为O_2供应源的贡献也超过了RBC的源，即使HBV少量剂量，也表明HBV可能利用HBV用于O_2 Therapeatics。这些发现表明，对HBV的物理化学特性操纵可以使量身定制的人工氧载体用于许多临床适应症。

项目成果

Blood substitutes

• DOI: 10.1111/j.1469-185x.1937.tb01222.x
• 发表时间: 1937-01-01
• 期刊: BIOLOGICAL REVIEWS OF THE CAMBRIDGE PHILOSOPHICAL SOCIETY
• 作者: Amberson, WR