Microhemodynamic properties and gas reactions of abnormal RBCs in narrow tubes

使用狭窄的毛细管（内径25 μ m），当用升高聚集的异常红细胞灌注时，测量壁附近的无细胞层（CFL）宽度。高分子右旋糖酐诱导红细胞聚集。增加聚集水平显示更宽的CFL和增加的红细胞碰撞管壁的发生率。流动过程中红细胞与内皮细胞的接触会产生很强的切应力，这可能会影响NO的产生。另一方面，较宽的CFL会减少红细胞对NO的消耗。还预期更宽的CFL将影响从红细胞到血管壁的氧卸载。CFL增加将增强血浆撇除，这也可能诱导不均匀的血液供应和降低的组织氧合。预期分散在无细胞层中的亚微米尺寸的人造红细胞的存在将改善组织氧合。

Using a narrow capillary tube (inner diameter 25 um), the cell free layer (CFL) width nearby the wall was measured when perfused with abnormal erythrocytes of elevated aggregation. Aggregation of erythrocyte was induced by the addition of high molecular weight dextran. Increasing the aggregation level showed a wider CFL and an increased incidence of collision of erythrocytes to the tube wall. Contacts of erythrocytes on the endothelum during flow induces a strong shear stress that may influence on the production of NO. On the other hand, a wider CFL will decrease NO consumption by erythrocytes. It is also expected that a wider CFL would affect on the oxygen unloading from erythrocytes to a vascular wall. Increased CFL will enhance plasma skimming which may also induce heterogeneous blood supply and decreased tissue oxygenation. It is expected that the presence of sub-micron sized artificial red cells, which disperse in the cell free layer, will improve the tissue oxygenation.