Microhemodynamic properties and gas reactions of abnormal RBCs in narrow tubes

窄管内异常红细胞的微血流动力学特性和气体反应

• 批准号: 24650301
• 负责人: SAKAI Hiromi
• 金额: $ 2.5万
• 依托单位: Nara Medical University (2013)Waseda University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650301/
• 关键词: ヘモレオロジー; 赤血球凝集; 微小循環; 国際研究者交流、シンガポール; 検査・診断システム

Using a narrow capillary tube (inner diameter 25 um), the cell free layer (CFL) width nearby the wall was measured when perfused with abnormal erythrocytes of elevated aggregation. Aggregation of erythrocyte was induced by the addition of high molecular weight dextran. Increasing the aggregation level showed a wider CFL and an increased incidence of collision of erythrocytes to the tube wall. Contacts of erythrocytes on the endothelum during flow induces a strong shear stress that may influence on the production of NO. On the other hand, a wider CFL will decrease NO consumption by erythrocytes. It is also expected that a wider CFL would affect on the oxygen unloading from erythrocytes to a vascular wall. Increased CFL will enhance plasma skimming which may also induce heterogeneous blood supply and decreased tissue oxygenation. It is expected that the presence of sub-micron sized artificial red cells, which disperse in the cell free layer, will improve the tissue oxygenation.

采用内径为25 μ m的窄毛细管，测量了在灌注异常聚集性升高的红细胞时，近壁游离细胞层（CFL）的宽度。高分子量右旋糖酐的加入诱导红细胞聚集。增加聚集水平可使CFL变宽，红细胞与管壁碰撞的发生率增加。红细胞在血流过程中与内皮的接触会产生强烈的剪切应力，从而影响一氧化氮的产生。另一方面，更宽的CFL会减少红细胞对NO的消耗。更宽的CFL也会影响红细胞向血管壁的氧卸载。CFL的增加会增强血浆脱脂，这也可能导致血液供应不均匀和组织氧合降低。预计分散在无细胞层中的亚微米大小的人造红细胞的存在将改善组织氧合。

项目成果

Qunantification of cell-free layer width changes by erythrocyte aggregation in a 25-µm tube

通过 25 µm 管中红细胞聚集对无细胞层宽度变化进行定量

• 作者: B. Namgung;H. Sakai;S. Kim
• 通讯作者: S. Kim

人工赤血球(Hb小胞体)の新しい利用法の深求と、シンガポールにおける融合研究拠点の形成

深入探索人造红细胞（Hb内质网）新用途并在新加坡组建融合研究中心

• 发表时间: 2013
• 作者: B. Namgung;H. Sakai;S. Kim;酒井宏水
• 通讯作者: 酒井宏水

人工赤血球の新しい利用法の深求とシンガポールにおける融合研究拠点の形成

深入探索人造红细胞新用途并在新加坡组建融合研究中心

• 作者: Miura M;Nagasaka M;Ito O;Kawai Y;Kohzuki M;酒井宏水
• 通讯作者: 酒井宏水

Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics

细胞型血红蛋白氧载体（血红蛋白囊泡）作为输血替代品和氧治疗

• 发表时间: 2012
• 期刊: Current Drug Discovery Techonl.
• 作者: 中澤公孝;一寸木洋平;中澤公孝;中澤公孝;中澤公孝;中澤公孝;中澤公孝;H. Sakai
• 通讯作者: H. Sakai

奈良県立医科大学化学教室

奈良医科大学化学系