Analysis of radiation-induced somatic mutation by using a gene knockout

使用基因敲除分析辐射诱导的体细胞突变

基本信息

  • 批准号:
    16310035
  • 负责人:
  • 金额:
    $ 8.32万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2006
  • 项目状态:
    已结题

项目摘要

DNA double-strand break (DSB), which is efficiently induced by ionizing radiation, represents the most potentially serious damage to genome maintenance. There are at least two pathways which can repair DSBs: non-homologous end-joining (NHEJ) and homologous recombination (HR). The Nbs1 gene is known to code for a protein involved in the hereditary cancer-prone disease, Nijmegen breakage syndrome, and act in regulation of DSB response. This gene is conserved in animals and fungi, but no plant homolog is known. We reported the cloning of a homolog of Nbs1 isolated from higher plants. The Nbs1 proteins from both Arabidopsis thaliana and Oryza sativa are smaller in size than animal or yeast Nbs1, but both contain the conserved Nbs1 conserved domains in orientations similar to what is seen in animal Nbs1. The OsNbs1 protein interacted not only with plant Mre11, but also with animal Mre11. These results suggest that plant Nbs1 could participate in a Rad50/Mre11/Nbs1 complex, and could be essential for the regulation of DNA recombination and DNA damage responses. We also trying to establish a novel detection system for somatic mutation combined with the gene targeting of a specific protein that functions in DSB response. These investigation is in progress.
DNA双链断裂(DSB)是电离辐射诱导的一种重要的基因组损伤。至少有两种途径可以修复DSB:非同源末端连接(NHEJ)和同源重组(HR)。已知Nbs 1基因编码一种与遗传性癌症易感性疾病Nijmegen断裂综合征有关的蛋白质,并参与DSB反应的调节。该基因在动物和真菌中是保守的,但没有已知的植物同源物。我们报道了一个从高等植物中分离的Nbs 1同源基因的克隆。来自拟南芥和水稻的Nbs 1蛋白在大小上比动物或酵母Nbs 1小,但两者都含有保守的Nbs 1保守结构域,其方向与在动物Nbs 1中所见的相似。OsNbs 1蛋白不仅与植物Mre 11相互作用,而且与动物Mre 11相互作用。这些结果表明,植物Nbs 1可以参与Rad 50/Mre 11/Nbs 1复合体,并可能是必要的DNA重组和DNA损伤反应的调节。我们还试图建立一种新的检测系统的体细胞突变结合基因打靶的一个特定的蛋白质,在DSB反应的功能。这些调查正在进行中。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TAUCHI Hiroshi其他文献

TAUCHI Hiroshi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TAUCHI Hiroshi', 18)}}的其他基金

Analysis of radiation-induced DNA double strand break repair during cell cycle
细胞周期中辐射诱导的 DNA 双链断裂修复分析
  • 批准号:
    25550023
  • 财政年份:
    2013
  • 资助金额:
    $ 8.32万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Study on DNA repair mechanism and mutagenesis using novel cell lines
利用新型细胞系研究DNA修复机制和诱变
  • 批准号:
    19310035
  • 财政年份:
    2007
  • 资助金额:
    $ 8.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on repair mechanism of DMA double-strand breaks induced by ionizing radiation using a gene targeting technique
基因打靶技术研究电离辐射诱导的DMA双链断裂修复机制
  • 批准号:
    14580560
  • 财政年份:
    2002
  • 资助金额:
    $ 8.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Role of NBS1 in Repair of DNA damage Induced by Ionizing Radiation
NBS1 在修复电离辐射引起的 DNA 损伤中的作用
  • 批准号:
    12680543
  • 财政年份:
    2000
  • 资助金额:
    $ 8.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Albumin hitchhiking siRNAs for gene targeting in aged brain
白蛋白搭便车 siRNA 用于老年大脑基因靶向
  • 批准号:
    10611521
  • 财政年份:
    2022
  • 资助金额:
    $ 8.32万
  • 项目类别:
Albumin hitchhiking siRNAs for gene targeting in aged brain
白蛋白搭便车 siRNA 用于老年大脑基因靶向
  • 批准号:
    10467737
  • 财政年份:
    2022
  • 资助金额:
    $ 8.32万
  • 项目类别:
Basic research on nucleic acids therapeutics for IL-12p40 gene targeting
IL-12p40基因靶向核酸治疗的基础研究
  • 批准号:
    20K16082
  • 财政年份:
    2020
  • 资助金额:
    $ 8.32万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
A new bladder cancer model based on tissue reprogramming and gene targeting
基于组织重编程和基因靶向的新膀胱癌模型
  • 批准号:
    10084281
  • 财政年份:
    2020
  • 资助金额:
    $ 8.32万
  • 项目类别:
A new bladder cancer model based on tissue reprogramming and gene targeting
基于组织重编程和基因靶向的新膀胱癌模型
  • 批准号:
    9896122
  • 财政年份:
    2020
  • 资助金额:
    $ 8.32万
  • 项目类别:
Multiplex gene targeting with CRISPR/Cas9 using single guide RNAs
使用单引导 RNA 通过 CRISPR/Cas9 进行多重基因打靶
  • 批准号:
    540963-2019
  • 财政年份:
    2019
  • 资助金额:
    $ 8.32万
  • 项目类别:
    University Undergraduate Student Research Awards
Development of conditional and inducible gene targeting tools in rabbits
兔子条件性和诱导性基因打靶工具的开发
  • 批准号:
    9317588
  • 财政年份:
    2017
  • 资助金额:
    $ 8.32万
  • 项目类别:
Core B: Transgenic and Gene-Targeting Institutional Facility
核心 B:转基因和基因靶向机构设施
  • 批准号:
    10215556
  • 财政年份:
    2017
  • 资助金额:
    $ 8.32万
  • 项目类别:
Exploring function of mu opioid receptor splice variants in rat by gene targeting
通过基因打靶探索大鼠μ阿片受体剪接变体的功能
  • 批准号:
    9312277
  • 财政年份:
    2016
  • 资助金额:
    $ 8.32万
  • 项目类别:
Mechanistic Study and Gene Targeting to Block HIV Assembly by IN-Binding Protein
通过 IN 结合蛋白阻断 HIV 组装的机制研究和基因靶向
  • 批准号:
    9377499
  • 财政年份:
    2016
  • 资助金额:
    $ 8.32万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了