Fabrication of Hierarchic DNA Nano-Array based on Stretched DNA

基于拉伸 DNA 的分层 DNA 纳米阵列的制造

• 批准号: 16310079
• 负责人: IJIRO Kuniharu
• 金额: $ 9.92万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310079/
• 关键词: DNA; Single Molecule; Monolayer; DNA Array; LB Method; FISH Method; Nano Structure

The DNA micro-array method is very important fundamental technique. For high-through put analysis, the high density and high degree of integration of a DNA array is required. However, the DNA spots density less than 20 mm cannot be achieved by using conventional photolithography and spotter. Therefore we attempted to develop DNA arrangement technology with 100 nm distance by the FISH (Fluorescence In-situ Hybridization) method based on a stretched single DNA molecule as a template for the DNA array.We demonstrated that oligonucleotides whose sequences were 40mer and overlapped by 20mer were formed multi-assemblies. The structural change from a linear to a circle was dependent on metal cations. This result suggested that we could design DNA structures by adding cations. In order to control the DNA structures, we investigated the concentration of cations to induce structural changes and sequences to interact with cations specifically. Four sorts of oligonucleotides, which contained A-tract or not, or A-tract position was different, were prepared. Each of the multi-assemblies provided different electrophoretic gel patterns in TE, TE containing Na^+ or Mg^<2+>. It concluded that metal ion changed the structure of DNA multi-assemblies from linear to circular forms.

DNA微阵列技术是一项非常重要的基础技术。对于高通量分析，需要DNA阵列的高密度和高集成度。然而，小于20 mm的DNA斑点密度不能通过使用常规光刻和点样器来实现。因此，我们尝试以拉伸后的单DNA分子为模板，通过荧光原位杂交（FISH）技术，发展了100 nm距离的DNA阵列技术，证明了40 mer序列重叠20 mer的寡核苷酸可以形成多个组装体。从线性到圆形的结构变化依赖于金属阳离子。这一结果表明，我们可以通过添加阳离子来设计DNA结构。为了控制DNA的结构，我们研究了阳离子的浓度，以诱导结构的变化和序列与阳离子特异性地相互作用。制备了四种不同的寡核苷酸，包括A片段的存在和不存在，以及A片段的位置不同。每个多组装体在TE、含Na^+或Mg^<2+>的TE中提供不同的电泳凝胶图案。结果表明，金属离子使DNA多组装体的结构由线形变为环状。

项目成果

Fabrication of Silver Nanowires by Selective Electroless Plating of DNA Stretched Using the LB Method

• DOI: 10.1246/cl.2005.112
• 发表时间: 2005-01
• 期刊: Chemistry Letters
• 影响因子: 1.6
• 作者: Y. Hashimoto;Y. Matsuo;K. Ijiro

Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir-Blodgett method.

• DOI: 10.1016/j.colsurfb.2004.10.025
• 发表时间: 2005-02
• 期刊: Colloids and surfaces. B, Biointerfaces
• 作者: Y. Matsuo;K. Ijiro;M. Shimomura