Fabrication of Hierarchic DNA Nano-Array based on Stretched DNA

点击翻译按钮获取中文摘要

The DNA micro-array method is very important fundamental technique. For high-through put analysis, the high density and high degree of integration of a DNA array is required. However, the DNA spots density less than 20 mm cannot be achieved by using conventional photolithography and spotter. Therefore we attempted to develop DNA arrangement technology with 100 nm distance by the FISH (Fluorescence In-situ Hybridization) method based on a stretched single DNA molecule as a template for the DNA array.We demonstrated that oligonucleotides whose sequences were 40mer and overlapped by 20mer were formed multi-assemblies. The structural change from a linear to a circle was dependent on metal cations. This result suggested that we could design DNA structures by adding cations. In order to control the DNA structures, we investigated the concentration of cations to induce structural changes and sequences to interact with cations specifically. Four sorts of oligonucleotides, which contained A-tract or not, or A-tract position was different, were prepared. Each of the multi-assemblies provided different electrophoretic gel patterns in TE, TE containing Na^+ or Mg^<2+>. It concluded that metal ion changed the structure of DNA multi-assemblies from linear to circular forms.