Fabrication of DNA conjugated gold nanoparticle for single electron devices

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DNA-based nanoparticle assembly is powerful tool for fabrication of nanostructures such as nano-patterning, whereas it is required to prepare nanoparticles in which a discrete and known number of single-stranded oligonucleotides are attached by thiol. We attempted to polymerize single primer-template DNA bound to Au nanoparticles by DNA polymerase, Klenow Fragment exo- (KF-), depending on reaction time. The AFM observation and gel electrophoresis suggest that KF- extended the short primer template bound to Au nanoparticle.