Prediction of radiosensitivity using phosphorylation of histone H2AX in human tumor cell lines

利用人类肿瘤细胞系中组蛋白 H2AX 的磷酸化预测放射敏感性

• 批准号: 21591608
• 负责人: SASAI Keisuke
• 金额: $ 2.83万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591608/
• 关键词: リン酸化H2AX; DNA二重鎖切断; 放射線感受性予測; DNAプロイディ; 細胞生存率; アポプトーシス; DNA ploidy; 放射線感受性; 蛍光免疫染色

To develop a new predictive assay for radiosensitivity, we tested the relationship between cell survival and expression of phosphorylation of histone H2AX(γH2AX) foci using 7 different human cell lines. There was not a good correlation between the γH2AX foci frequency and surviving fraction after irradiation. When we normalized theγH2AX foci frequency by DNA content, and introduced the frequency of apoptosis to the analysis, there was a good correlation between the cell survival and the combined frequency of apoptosis and γH2AX foci in 6 cell lines except for one.

为了建立一种新的放射敏感性预测方法，我们使用7个不同的人类细胞系，测试了组蛋白H_2AX(γH_2AX)焦点的磷酸化表达与细胞存活的关系。γ-H_2AX焦点频率与照射后存活分数之间无明显相关性。将γH_2AX焦点频率归一化，并引入细胞凋亡率进行分析，除1株细胞外，其余6个细胞系的细胞存活率与细胞凋亡率和γH_2AX焦点频率的组合有很好的相关性。

项目成果

Long-term outcomes of three-dimensional conformal radiation therapy combined with neoadjuvant hormonal therapy in Japanese patients with locally advanced prostate cancer.

三维适形放射治疗联合新辅助激素治疗日本局部晚期前列腺癌患者的长期结果。

• 发表时间: 2011
• 期刊: Int J Clin Oncol.
• 作者: Sakamoto M;Mizowaki T;Mitsumori M;Takayama K;Sasai K;Norihisa Y;Kamoto T;Nakamura E;Ogawa O;Hiraoka M
• 通讯作者: Hiraoka M

Elevation of serumcarcinoembryonic antigen level in a patient with hypothyroidism after radiation therapy for cervical esophageal cancer

宫颈食管癌放射治疗后甲减患者血清癌胚抗原水平升高

• 发表时间: 2010
• 期刊: Int J Clin Oncol
• 影响因子: 3.3
• 作者: Kawaguchi G;Abe E;Sasamoto R;Sasai K.
• 通讯作者: Sasai K.

Full-dose capecitabine with loradiotherapy : one of the treatment options for inoperable T4 breast cancer

全剂量卡培他滨联合劳放射治疗：无法手术的 T4 乳腺癌的治疗选择之一

• 发表时间: 2011
• 期刊: Jpn J Radiol. 2
• 作者: Hirowatari H;Karasawa K;Izawa H;Ito K;Sasai K;Furuya T;Ozawa S;Arakawa A;Orihata G;Saito M.
• 通讯作者: Saito M.

Effect of dose fractionation on pulmonary complications during total body irradiation

剂量分割对全身照射期间肺部并发症的影响

• 发表时间: 2011
• 期刊: J Radiat Resarch
• 作者: Izawa H;Hirowatari H;Yahata Y;Hamano Y;Ito K;Saito AI;Yamamoto H;Miura K;Karasawa K;Sasai K.
• 通讯作者: Sasai K.

The predominant expression of hepatocyte nuclear factor 4α (HNF4α) in thyroid transcription factor-1 (TTF-1)-negative pulmonary adenocarcinoma

• DOI: 10.1111/j.1365-2559.2011.03764.x
• 发表时间: 2011-02-01
• 期刊: HISTOPATHOLOGY
• 影响因子: 6.4
• 作者: Kunii, Ryosuke;Jiang, Shuying;Naito, Makoto
• 通讯作者: Naito, Makoto