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Development of functional biomaterials using laminin active peptides

利用层粘连蛋白活性肽开发功能性生物材料

基本信息

批准号：
21K06563
负责人：
野水基義
金额：
$ 2.66万
依托单位：
Tokyo University of Pharmacy and Life Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

基底膜を模倣したバイオマテリアルの開発を目的として研究を行った。個体の発生や再生において細胞外環境を提供する基底膜を模倣したバイオマテリアルは、再生医療を目的とした組織工学において必須のものである。我々は長年にわたり基底膜の主要成分のラミニンの機能部位の解明を3000種類以上の合成ペプチドを用いて網羅的に行い、約100種類の活性ペプチドを同定してきた。また、活性ペプチドを高分子多糖マトリックスに結合させたペプチド-マトリックスが細胞培養の足場材料として有用であることを示してきた。本研究は、我々が同定したラミニン活性ペプチドを複数種類混合して固定化したペプチド-マトリックスの機能性バイオマテリアルとしての応用を目的としている。本研究ではまずこれまでの実験において活性のあった約100種類のペプチドの中でペプチド-マトリックスにした場合に顕著な活性を示すペプチドを再評価し、約10種類のペプチドを得た。現在、この10種類に関して様々な細胞を用いて細胞接着や培養の評価を行っている。また、インテグリンαvβ5に対して特異的に作用するArg-Gly-Asp配列を含むラミニンα5鎖のペプチドを同定し、これを用いたペプチド-マトリックスがiPS細胞の培養に応用してきた。今後の実験でさらに細胞培養に有効なペプチド-マトリックスをみいだし、機能性バイオマテリアルとして開発する。さらに、ラミニン由来のアミロイド線維形成ペプチドを同定し、これに活性ペプチドを付加することにより、新規機能性バイオマテリアルの開発を行った。これらバイオマテリアルは、iPS細胞やES細胞の培養および分化誘導、3次元培養や器官培養を可能にするもので、組織工学や再生医療の発展に大きく寄与するものである。

The basement membrane model was used for the purpose of the study. In the extracellular environment, the basement membrane model is available. The purpose of regenerative medicine is to ensure that the tissue engineering and science system must be optimized. Over the years, we have been working on the major components of the basement membrane, which are the main components of the basement membrane system. We have been working on the major components of the basement membrane, which are the main components of the basement membrane. We have been working over the years to identify the major components of the basement membrane, which are the main components of the basement membrane system, the most important components of the basement membrane. The materials used in the field of cell culture are used to show that they are useful. The purpose of this study is to determine the performance of the hybrid system, such as the immobilization of the immobilized system, the activation of the complex, the immobilization of the immobilized system, the activation of the complex, the immobilization of the immobilized system, the activation of the complex, the immobilization of the immobilized system, the activation of the complex, the immobilization of the immobilized system. The purpose of this study is to compare the results of this study. In this study, the results of this study show that there are about 10 percent of the data in this study. In this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of this study are as follows: in this study, the results of Now, in the last ten years, we have to use the same cell to make sure that we use the same cell to make sure that we have a problem with each other. The function of the special device, the function of the device, the Arg-Gly-Asp, the function, the function, the location, the In the future, there will be a wide range of cell culture programs, such as health care, functional training, and so on. The cause of the accident and the cause of the disease is that the agreement is the same, the activity is the same, and the new regulation mechanism is required to start the operation. The tissue engineering and regenerative medicine experiments have been carried out in the tissue engineering and regenerative medicine exhibition, the iPS cell culture, the iPS cell culture, the tissue engineering and regenerative medicine, and the tissue engineering, tissue engineering and regenerative medicine.