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Development of functional Ti implants with release of growth factors

开发释放生长因子的功能性钛植入物

基本信息

批准号：
16390557
负责人：
YOSHIDA Yasuhiro
金额：
$ 8.83万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390557/
关键词：
implant titanium polymer growth factor XPS SPR QCM phosphorylation ポリリン酸

项目摘要

In order to develop functional Ti implants with release of growth factors, biodegradable materials as a carrier have to adhere strongly to Ti surface. Lactide copolymers synthesized in this study hardly adhered to TI surface.Ti plates were treated with 0.1 and 1 N hydrochloric acid, 37wt% phosphoric acid or kept untreated. The tensile bond strength of the composite cement to the untreated and pre-treated Ti plates was determined without and after 20,000 thermo-cycles. XPS was used to chemically analyze the effect of the three Ti pre-treatments, as well as the interaction of 10-methacryloxydecyl dihydrogen phosphate (10-MDP) with Ti treated with 1 N HCl. Although no significant difference in immediate tensile bond strength was measured, thermo-cycling significantly decreased the bond strength of all experimental groups except for Ti treated with 1 N HCl. XPS demonstrated that H_3PO_4 was strongly adsorbed on the Ti surface. These results clearly suggest that phosphorylation was effective to get strong attachment to the Ti surface. Ti pre-treated with 1 N HCl improved the adsorption of 10-MDP as compared to untreated Ti.Cleaned Ti disks were immersed into three different concentrations of polyphosphoric acid solution (0.1,1 and 10 wt%) for 24 hours at 37℃. Ti immersed in distilled water for 24 hours at 37℃ served as control. Degrees of cell attachment (1,3,5 hours after cell seed) and proliferation (1,3,5 and 7 days after cell seed) on each treated Ti disk were evaluated by MTS assay. A significantly higher cell attachment was found on Ti treated with polyphosphoric acid in contrast to untreated Ti (control) for all three culture periods. MTS assay also revealed that cell proliferation levels significantly increased following a polyphosphoric-acid dose dependency. It was concluded that polyphosphoric-acid treatment of Ti enhanced the attachment and proliferation of hBMSCs.

为了开发具有生长因子释放功能的钛植入物，生物可降解材料作为载体必须牢固地粘附在钛表面。用0.1和1 N盐酸、37wt%磷酸处理钛板或不处理钛板，所得的丙交酯共聚物几乎不粘附钛板。在没有和经过20，000次热循环后，测定复合骨水泥与未处理和预处理钛板的拉伸粘合强度。XPS用于化学分析三种Ti预处理的效果，以及10-甲基丙烯酰氧基癸基磷酸二氢酯（10-MDP）与用1 N HCl处理的Ti的相互作用。虽然没有显着差异，立即拉伸粘结强度进行了测量，热循环显着降低的粘结强度的所有实验组，除了钛处理1 N盐酸。XPS分析表明，H_3PO_4在Ti表面有较强的吸附。这些结果清楚地表明磷酸化有效地获得与Ti表面的强附着。用1 N HCl预处理的钛片对10-MDP的吸附性能有明显的提高。将钛浸入37℃蒸馏水中24小时作为对照。通过MTS试验评价细胞在每个处理的钛片上的附着程度（细胞接种后1、3、5小时）和增殖程度（细胞接种后1、3、5和7天）。一个显着更高的细胞附着被发现与未处理的Ti（对照）相比，在所有三个培养期的Ti与多磷酸。MTS试验还显示，细胞增殖水平显着增加后，多聚磷酸剂量依赖性。结果表明，多聚磷酸处理的钛促进了hBMSCs的贴壁和增殖。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nano-technological control of biomaterial-hard tissue interfaces

生物材料-硬组织界面的纳米技术控制

DOI：
发表时间：
2005
期刊：
Journal of Hard Tissue Biology 14(2)
影响因子：
0
作者：
Lewis JB;Yoshida.Y.et al.
通讯作者：
Yoshida.Y.et al.

Effect of 4-MET- and 10-MDP-based primers on resin bonding to titanium

DOI：
10.4012/dmj.25.120
发表时间：
2006-03-01
期刊：
DENTAL MATERIALS JOURNAL
影响因子：
2.5
作者：
Tsuchimoto, Youhei;Yoshida, Yasuhiro;Kuboki, Takuo
通讯作者：
Kuboki, Takuo

Effect of surface pre-treatment on durability of resin-based cements bonded titanium.

表面预处理对树脂基水泥粘结钛耐久性的影响。

DOI：
发表时间：
2006
期刊：
Dental Materials 22
影响因子：
0
作者：
Tsuchimoto;Y.;Yoshida;Y.;Takeuchi;M.;Mine;A.;Yatani;H.;Tagawa;Y.;Van Meerbeek;B.;Suzuki;K.;Kuboki;T.
通讯作者：
T.

Adsorption of polyphosphoric acid to titanium surface and its effect on hBMSC attachment.

多磷酸在钛表面的吸附及其对 hBMSC 附着的影响。