Glyco-engineering based on enzymatic polymer reactions

基于酶促聚合物反应的糖工程

• 批准号: 17350053
• 负责人: SHODA Shin-ichiro
• 金额: $ 8.45万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17350053/
• 关键词: Enzymatic polymer reaction; Sulfation; Phosphorylation; Sulfatase; Phosphatase; Cellulose biding domain; Phytase; Chitin biding domain; 糖加水分解酵素; オリゴ糖; 水溶性脱水縮合剤; 糖供与体; 糖受容体; 配糖化反応; N-イソブロヒルアクリルアミド; 熱応答性高分子; 糖オルトエステル; グリコシル化; アセチルエステラーゼ

1. Development of New Method for Direct Activation of the Anomeric Hydroxy GroupDirect synthesis of various important intermediates, sugar oxazolines, 1,6-anhydro sugars, 1-thioglycosides, and glycosyl azides, has been achieved in aqueous media by using a dimethylimidazolinium salt as condensation agent.2. Facile Synthesis of Sugar Orthoesters and Screening of HydrolasesA new method for synthesis of sugar orthoesters promoted by potassium fluoride has been developed. Enzymes that recognize a sugar orthoester have been screened.3. Synthesis of Cyclic Sugar Carbonate and Its Chemical BehaviorA cyclic sugar carbonate was prepared and its chemical behavior, for example, the stability against acid or base, reactivity towards various nucleophiles, has been investigated.4. Development of Enzymatic Polymer ReactionsA new method for modifying polysaccharide chains by enzymes has been developed. The sulfation and phosphorylation of chitooligosaccharides and amylase has been demonstrated.5. Acceleration of Enzymatic Polymer ReactionsIn order to facilitate the above enzymatic polymer reaction, the enzyme catalysts have been modified by introducing a biding domain for the corresponding polysaccharides.

1.异头羟基直接活化新方法的开发利用二甲基咪唑啉盐作为缩合剂，在水介质中直接合成了糖恶唑啉、1，6-脱水糖、1-硫代糖苷和糖基叠氮化物等重要中间体.糖原酸酯的简易合成及水解酶的筛选研究了以氟化钾为促进剂合成糖原酸酯的新方法。已经筛选出识别糖原酸酯的酶。环状糖碳酸酯的合成及其化学性质研究了环状糖碳酸酯的合成及其化学性质，如对酸、碱的稳定性，对各种亲核试剂的反应性等.酶促高分子反应的研究开发了一种利用酶修饰多糖链的新方法。研究了壳寡糖和淀粉酶的硫酸化和磷酸化反应.酶促聚合反应的加速为了促进上述酶促聚合反应，通过引入相应多糖的结合结构域来修饰酶催化剂。

项目成果

Colorimetric Assay for Evaluating Glycosyl Fluoride-hydrolyzing Activity of Glycosidase by Using Alizarin Complexon Reagent

茜素络合剂比色法评价糖苷酶的糖基氟水解活性

• 发表时间: 2007
• 期刊: Chem. Lett 36
• 作者: Matsumoto;K.;Y.Matsumura;O.Onomura;O.Onomura;Toshio Masuda;Toshiki Kono;T.Masuda;T.Kono;Yosuke Taniguchi;Yuichi Shida;Toshikazu Sakaguchi;Toru Katsumata;Toshikazu Sakaguchi;Y.Taniguchi;Y.Shida;T.Sakaguchi;T.Katsumata;T.Masuda;T.Masuda;Toru Katsumata;Toshikazu Sakaguchi;Yosuke Taniguchi;Yuichi Shida;Yuichi Shida;Toshikazu Sakaguchi;Roy D.Raharjo;Y.Shida;T.Sakaguchi;R.D.Raharjo;Yuichi Shida;Toshikazu Sakaguchi;Roy D.Raharjo;Toshikazu Sakaguchi;Toshio Masuda;増田俊夫;Tomonari Tanaka;Tomonari Tanaka;Shin-ichiro Shoda
• 通讯作者: Shin-ichiro Shoda

Chemo-enzymatic synthesis of novel oligo-N-acetyllactosamine derivatives having a β (1-4)-β (1-6) repeating unit by using transition state analogue substrate

使用过渡态类似物底物化学酶法合成具有 β (1-4)-β (1-6) 重复单元的新型低聚-N-乙酰基乳糖胺衍生物

• 发表时间: 2006
• 期刊: Cellulose 13
• 作者: 石原正規;小林厚志;野口真人;正田晋一郎;S.Shoda
• 通讯作者: S.Shoda

糖オキサゾリン誘導体の一段階製造方法

一种糖恶唑啉衍生物的一步生产方法

• 发表时间: 2008

アンヒドロ糖及びその製造方法

脱水糖及其制造方法

• 发表时间: 2007

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄