Investigation of the Mechanism of the Production and the Evaluation as a Cell Culture Support of Polymer Porous Medium with Controlled 3-Dimensional Structure

本研究主要研究了超临界二氧化碳中聚合制备聚合物多孔介质的方法。1.聚合物多孔介质三维结构的控制在超临界二氧化碳中进行了苯乙烯和丙烯腈的交联聚合，研究了聚合物多孔介质三维结构的控制方法。聚合在各种压力、温度和浓度条件下进行。研究了聚合条件与多孔介质粒径、一次粒子粒径、比表面积、细孔孔径分布的关系。2.聚合物多孔介质的制备机理推测聚合物在聚合场中的溶解度影响聚合物多孔介质的制备机理。基于直到聚合物从聚合溶液中沉积出来的时间，定量显示聚合物的溶解度。 关于我们 3.搅拌式超临界聚合反应器制备聚合物多孔介质研究了搅拌式超临界聚合反应器制备聚合物多孔介质的工业化生产。建立了苯乙烯多孔介质的制备工艺。研究了搅拌桨转速对聚合反应速率和结构的影响。4.聚合物粒子在多孔介质中的成型及多孔介质的表面处理、洗涤研究了用超临界二氧化碳处理聚合物粒子在多孔介质上成型的方法。研究了多孔介质的表面处理和超临界CO2清洗。5.人前列腺癌细胞在多孔介质中的培养以聚苯乙烯和聚丙烯腈多孔介质为载体进行了人前列腺癌细胞的培养。在所有的多孔介质中，细胞都在向多孔介质内部增加，证实了本研究中的聚合物多孔介质可以将其用作细胞的培养支持物。少

In this study, the production of the polymer porous medium by polymerization in supercritical carbon dioxide was investigated.1.Control of 3-dimensional structure of the polymer porous mediumThe cross linked polymerization of styrene and acrylics nitril was performed in supercritical carbon dioxide, and the control method of the 3-dimensional structure of the polymer porous medium was investigated. Polymerization was performed on various conditions of pressure, temperature and concentration. The relation between the polymerization conditions and the diameter of the porous medium, the diameter of a primary particle, specific surface area and the diameter distribution of fine pores was shown.2.Mechanism of the polymer porous medium productionIt is guessed that the solubility of the polymer in polymerizing field influences on the mechanism of polymer porous medium production. Based on time until polymer deposits out of polymerization solution, the solubility of polymer was shown quantitat … More ively, and the influence polymerization condition on structure was explained.3.Production of the polymer porous medium using stirred type supercritical polymerization reactorThe industrial production of the polymer porous medium using stirred type supercritical polymerization reactor was considered. The procedure which produces a styrene porous medium was established. The influence of the impeller rotations number on the rate of polymerization and structure was shown.4.Molding to polymer porous medium of the polymer particles and surface treatment, washing of the porous mediumThe method of molding polymer particles on the porous medium by treating with supercritical carbon dioxide was discussed. Furthermore, the surface treatment and washing of the polymer porous medium using supercritical carbon dioxide were studied.5.Cultivation of human prostatic cancer cell using polymer porous mediumThe human prostatic cancer cell culture using the polystyrene and the poly acrylics nitrile porous medium as a culture support was performed. In all porous mediums, the cell was increasing to the inside of the porous medium, and it was confirmed that the polymer porous medium in this study can use it as a cell a culture support. Less