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Investigation of the Mechanism of the Production and the Evaluation as a Cell Culture Support of Polymer Porous Medium with Controlled 3-Dimensional Structure

可控三维结构聚合物多孔介质的生产机理研究及其作为细胞培养支持物的评价

基本信息

批准号：
17360381
负责人：
NISHI Kazuhiko
金额：
$ 5.25万
依托单位：
Yokohama National University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

In this study, the production of the polymer porous medium by polymerization in supercritical carbon dioxide was investigated.1.Control of 3-dimensional structure of the polymer porous mediumThe cross linked polymerization of styrene and acrylics nitril was performed in supercritical carbon dioxide, and the control method of the 3-dimensional structure of the polymer porous medium was investigated. Polymerization was performed on various conditions of pressure, temperature and concentration. The relation between the polymerization conditions and the diameter of the porous medium, the diameter of a primary particle, specific surface area and the diameter distribution of fine pores was shown.2.Mechanism of the polymer porous medium productionIt is guessed that the solubility of the polymer in polymerizing field influences on the mechanism of polymer porous medium production. Based on time until polymer deposits out of polymerization solution, the solubility of polymer was shown quantitat … More ively, and the influence polymerization condition on structure was explained.3.Production of the polymer porous medium using stirred type supercritical polymerization reactorThe industrial production of the polymer porous medium using stirred type supercritical polymerization reactor was considered. The procedure which produces a styrene porous medium was established. The influence of the impeller rotations number on the rate of polymerization and structure was shown.4.Molding to polymer porous medium of the polymer particles and surface treatment, washing of the porous mediumThe method of molding polymer particles on the porous medium by treating with supercritical carbon dioxide was discussed. Furthermore, the surface treatment and washing of the polymer porous medium using supercritical carbon dioxide were studied.5.Cultivation of human prostatic cancer cell using polymer porous mediumThe human prostatic cancer cell culture using the polystyrene and the poly acrylics nitrile porous medium as a culture support was performed. In all porous mediums, the cell was increasing to the inside of the porous medium, and it was confirmed that the polymer porous medium in this study can use it as a cell a culture support. Less

本文研究了在超临界二氧化碳条件下聚合法制备聚合物多孔介质。高分子多孔介质三维结构的控制在超临界二氧化碳中进行了苯乙烯和丙烯酸腈的交联聚合，研究了高分子多孔介质三维结构的控制方法。聚合反应在不同的压力、温度和浓度条件下进行。得到了聚合条件与多孔介质直径、初级颗粒直径、比表面积和细孔直径分布的关系。聚合物多孔介质的产生机理推测聚合物在聚合场中的溶解度影响聚合物多孔介质的产生机理。以聚合物析出聚合溶液的时间为基础，定量表征了聚合物的溶解度，并说明了聚合条件对聚合物结构的影响。采用搅拌式超临界聚合反应器生产聚合物多孔介质研究了搅拌式超临界聚合反应器工业化生产聚合物多孔介质的方法。建立了苯乙烯多孔介质的制备工艺。研究了叶轮转速对聚合速率和结构的影响。对聚合物多孔介质的聚合物颗粒的成型及表面处理、多孔介质的洗涤进行了探讨，探讨了超临界二氧化碳处理在多孔介质上成型聚合物颗粒的方法。此外，还研究了超临界二氧化碳对聚合物多孔介质的表面处理和洗涤。高分子多孔介质培养人前列腺癌细胞用聚苯乙烯和聚丙烯腈多孔介质作为培养载体进行了人前列腺癌细胞的培养。在所有的多孔介质中，细胞都向多孔介质的内部增加，证实了本研究的聚合物多孔介质可以作为细胞的培养载体。少