Induction mechanism of and protection mechanism against the peroxidation of lipids in plant cells.

植物细胞脂质过氧化的诱导机制和保护机制。

• 批准号: 10660060
• 负责人: YAMAMOTO Yoko
• 金额: $ 2.24万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660060/
• 关键词: Apoptosis-like cell death; Aluminum tolerance; Aluminum toxicity; Pea root; Glutathione peroxidase; Caffeoyl putresine; Lipid peroxidation; Cultured tobacco cells; ヌクレオチド二リン酸キナーゼ; リンゴ酸脱水素酵素; エンドウ; グルタチオン; 根

Aluminum (Al) is thought to be a major factor to inhibit plant root growth in acid soils. Molecular mechanisms of Al toxicity and also of Al tolerance were investigated in cultured tobacco cells and pea roots.In tobacco cells in nutrient medium containing Fe ion (II, III), Al stimulated the Fe-mediated peroxidation of lipids which caused apoptosis-like cell death including internucleosomal breakage of DNA. Two-types of Al-tolerant tobacco cell lines were isolated and investigated specificity of the tolerance phenotype. Since they showed tolerance also to various kinds of reagents (HィイD22ィエD2OィイD22ィエD2, t-butyl hydroperoxide, A23187) which induce the peroxidation of lipids, it seems that they acquired a tolerant mechanism against the peroxidation of lipids. ON of the cell line accumulated phenolics, and the major one was identified as caffeoyl putresine by LC/MS and NMR. Caffeoyl putresine protected tobacco cells from the peroxidation of lipids cause by a combination of Al and Fe (II). This cell line also exhibit an enzymatic activity similar to glutathione peroxidase in animal systems.In pea roots, Al also enhanced the peroxidation of lipids, but did not require the coexistence of Fe. The peroxidation of lipids was one of the early events caused by Al. The Al-enhanced peroxidation of lipids was not cause of the inhibition of root elongation during Al treatment, but was partly a cause of the inhibition of root re-growth in Al-free medium.The results indicate that Al enhances the peroxidation of lipids in both cultured plant cells and roots, and suggest that antioxidants such as caffeoyl putresine and glutathione peroxidase seem to protect plant cells from the Al-enhanced peroxidation of lipids.

铝是酸性土壤中抑制植物根系生长的主要因素。以烟草细胞和豌豆根为材料，研究了铝毒和铝耐性的分子机制，结果表明：在含Fe（Ⅱ，Ⅲ）的培养基中，铝可引起烟草细胞脂质过氧化反应，引起烟草细胞核小体间DNA断裂，从而引起烟草细胞凋亡。本研究分离了两种耐铝烟草细胞系，并对其耐铝表型的特异性进行了研究。由于它们还对各种诱导脂质过氧化的试剂（H2O D22 H2O D22 H2O D2，叔丁基过氧化氢，A23187）显示耐受性，因此似乎它们获得了对脂质过氧化的耐受机制。通过LC/MS和NMR分析，确定了细胞中主要的酚类物质为咖啡酰腐胺。咖啡酰腐胺保护烟草细胞免受Al和Fe（II）组合引起的脂质过氧化作用。该细胞系还表现出与动物系统中的谷胱甘肽过氧化物酶类似的酶活性。在豌豆根中，Al也增强了脂质的过氧化作用，但不需要Fe的共存。脂质过氧化是铝引起的早期事件之一，铝促进的脂质过氧化不是铝处理抑制根伸长的原因，但在无铝培养基中，铝促进的脂质过氧化是抑制根再生长的部分原因。结果表明，铝促进了植物细胞和根的脂质过氧化，并表明，抗氧化剂，如咖啡酰腐胺和谷胱甘肽过氧化物酶似乎保护植物细胞从铝增强脂质过氧化作用。

项目成果

Yamaguchi, Y.: "Cell death process initiated by a combination of aluminum and iron in suspension-cultured tobacco cells (Nicotiana tabacum) : Apoptosis-like cell death mediated by calcium and proteinase"Soil Sci. Plant Nutr.. 45. 647-657 (1999)

Yamaguchi, Y.：“悬浮培养的烟草细胞（烟草）中铝和铁的组合引发的细胞死亡过程：钙和蛋白酶介导的细胞凋亡样细胞死亡”土壤科学。

山本洋子: "植物栄養・肥料の事典(第7章:活性酸素ストレス、酸ストレス.分担執筆)"朝倉出版 (印刷中).

山本洋子：《植物营养与肥料百科全书（第7章：活性氧胁迫、酸性胁迫。合著者）》朝仓出版社（正在印刷中）。

Chang, Y-C.: "Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum) cells treated with a combination of aluminium and iron"Plant Cell Environ.. 22. 1009-1017 (1999)

Chang, Y-C.：“用铝和铁组合处理的培养烟草 (Nicotiana tabacum) 细胞中铝在细胞壁果胶中的积累”Plant Cell Environ.. 22. 1009-1017 (1999)

Yamaguchi, Y., et al.: "Cell death process initiated by a combination of aluminum and iron in suspension-cultured tobacco cells (Nicotiana tabacum) : Apotosis-like cell death mediated by calcium and proteinase"Soil Sci. Plant Nutr.. 45. 647-657 (1999)

Yamaguchi，Y.，等人：“悬浮培养的烟草细胞（烟草）中铝和铁的组合引发的细胞死亡过程：钙和蛋白酶介导的细胞凋亡样细胞死亡”土壤科学。

Yamaguchi, Y., et al.: "Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension-cultured tobacco cells"Physiol. Plant.. 105. 417-422 (1999)

Yamaguchi，Y.，等人：“谷胱甘肽对悬浮培养的烟草细胞中铝和铁的组合引起的细胞毒性的保护作用”Physiol。