Analyses of aluminum-induced cell death via mitochondrial dysfunction in plant cells.

分析铝通过植物细胞线粒体功能障碍诱导的细胞死亡。

• 批准号: 14540595
• 负责人: YAMAMOTO Yoko
• 金额: $ 1.92万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540595/
• 关键词: Aluminum ion; Mitochondria; Cell death; Sucrose-H^+ cotransporter; Calcium ion; Second messenger; Peroxidase; Plant Cells; アルミニウム; カルシウム; 細胞伸長阻害; ショ糖吸収阻害; タバコ培養細胞; 水吸収阻害; アルミニウム耐性; 活性酸素; 細胞骨格; 植物; 浸透圧

In acidic soils, aluminum(Al) ion is a major factor to limit plant growth. We have previously found that Al ion on the cell surface triggers mitochondrial dysfunction, reactive oxygen species(ROS) production and eventually cell death. The purpose of this research was to investigate the molecular details of the Al toxicity pathway, and to identify molecules and/or genes related to Al tolerance.As an important early event, we found that Al inhibits sucrose uptake via sucrose-H^+ cotransporter in cultured tobacco cells. Since the inhibition of sucrose uptake and the inhibition of water uptake were induced simultaneously by Al, we concluded that the Al-induced inhibition of sucrose uptake leads to the inhibition of water uptake and eventually the inhibition of cell elongation. In addition, since the inhibition of sucrose uptake leads to a decrease in the supply of carbon source to mitochondria, which seems to lead to mitochondrial dysfunction and ATP depletion, and finally cell death.We also found a possibility that transient changes of cytosolic calcium ion concentration are related to Al-stress responses. We finally found that the peroxidase gene overexpressed in Al-tolerant tobacco cells is Al-tolerant gene, and that ATP content is one of determining factors of Al tolerance in pea roots.

在酸性土壤中，铝（Al）离子是限制植物生长的主要因素。我们之前发现细胞表面的铝离子会引发线粒体功能障碍、活性氧（ROS）产生并最终导致细胞死亡。本研究的目的是研究铝毒性途径的分子细节，并鉴定与铝耐受性相关的分子和/或基因。作为一个重要的早期事件，我们发现铝通过培养烟草细胞中的蔗糖-H^+协同转运蛋白抑制蔗糖摄取。由于铝同时诱导蔗糖摄取的抑制和水摄取的抑制，因此我们得出结论，铝诱导的蔗糖摄取抑制导致水摄取的抑制，并最终抑制细胞伸长。此外，由于蔗糖摄取的抑制导致线粒体碳源供应减少，这似乎导致线粒体功能障碍和ATP耗竭，最终导致细胞死亡。我们还发现细胞质钙离子浓度的瞬时变化可能与铝应激反应有关。我们最终发现耐铝烟草细胞中过表达的过氧化物酶基因是耐铝基因，并且ATP含量是豌豆根系耐铝的决定因素之一。

项目成果

Kobayashi, Y. et al.: "Studies on the mechanism of aluminum tolerance in pea (Pisum sativum L.) using aluminum-tolerant cultivar 'Alaska' and aluminum-sensitive cultivar 'Hyogo'."Soil Science and Plant Nutrition. (in press).

Kobayashi, Y. 等人：“利用耐铝品种‘阿拉斯加’和铝敏感品种‘兵库’研究豌豆 (Pisum sativum L.) 的铝耐受机制。”土壤科学与植物营养。

Aluminum-induced alterations of cytosolic calcium concentration and its correlation with other aluminum responses in cultured tobacco cells.

铝诱导的细胞质钙浓度的变化及其与培养烟草细胞中其他铝反应的相关性。

• 发表时间: 2005
• 期刊: Plant nutrition for food security, human health and environmental protection, eds Li, C. J., et al., Tsinghua University Press, Beijing
• 作者: Tsuchiya;Y.;et al.
• 通讯作者: et al.

Yamamoto, Y. et al.: "Oxidative stress triggered by aluminum in plant roots."Plant and Soil. 255. 239-243 (2003)

Yamamoto, Y. 等人：“植物根部铝引发的氧化应激。”植物和土壤。

Studies on the mechanism of aluminum tolerance in pea (Pisum sativum L.) using aluminum-tolerant cultivar 'Alaska' and aluminum-sensitive cultivar 'Hyogo'

• DOI: 10.1080/00380768.2004.10408468
• 发表时间: 2004-04-01
• 期刊: SOIL SCIENCE AND PLANT NUTRITION
• 影响因子: 2
• 作者: Kobayashi, Y;Yamamoto, Y;Matsumoto, H
• 通讯作者: Matsumoto, H

Recent advances in the physiological and molecular mechanism of Al toxicity and tolerance in higher plants. In Advances in Plant Physiology Vol.5.(A Hemantaranjan (ed))

高等植物铝毒性和耐受性的生理和分子机制的最新进展。

• 发表时间: 2003
• 作者: Panda;S. et al.;Hideaki Matsumoto
• 通讯作者: Hideaki Matsumoto