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Molecular analysis of the developmental mechanism of periodontal and oral diseases by the genome analysis of oral biofilm.

通过口腔生物膜的基因组分析对牙周和口腔疾病的发生机制进行分子分析。

基本信息

批准号：
17390485
负责人：
HAMADA Shigeyuki
金额：
$ 9.79万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390485/
关键词：
Porpyromonas gingival is fimbriae vesicle Streptococcus mutans glucose side chain genome Multilocus sequencing typing

项目摘要

Dental plaque has the properties of a biofilm, similar to other biofilms found in the body and the environment. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Oral biofilms are very heterogeneous in structure. However, the relationship between the genomic information and the development of oral and periodontal diseases are still ambiguous. In this study, we focused on two theme, (1) Analysis of genomic variation of oral streptococci (2) Pathogenic mechanism of Porphyromonas gingivalis against oral epithelial cells.(1) Analysis of genomic variation of oral streptococciS. mutans strains have been classified into four serotypes (c, e, f, and k). However, little is known about the S. mutans population, including the clonal relationships among strains of S. mutans. We have developed a multilocus sequence typing (MLST) scheme for S. mutans. Eight housekeeping gene fragments were sequenced … More from each of 102 S. mutans isolates. We identified 92 sequence types in these 102 isolates, indicating that S. mutans contains a diverse population. No geographic specificity was identified. However, the distribution of the collagen-binding protein gene (cnm) and direct evidence of mother-to-child transmission were clearly evident. We also performed the whole genome sequencing of the S. mutans NN2025 strain, and its genome had an extensive genomic arrangement across the replicore.(2) Pathogenic mechanism of Porphyromonas gingivalis against oral epithelial cells.Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence. Less

牙菌斑具有生物膜的性质，类似于身体和环境中发现的其他生物膜。现代分子生物学技术已经在牙齿生物膜中鉴定出大约1000种不同的细菌，是可以培养的细菌的两倍。口腔生物膜在结构上是非常不均匀的。然而，基因组信息与口腔和牙周疾病的发生发展之间的关系仍然是模糊的。本研究主要围绕两个方面展开：（1）口腔链球菌的基因组变异分析;（2）牙龈卟啉单胞菌对口腔上皮细胞的致病机制。(1)口腔链球菌基因组变异分析变异株被分为四种血清型（C、E、F和K）。然而，人们对S.变形链球菌种群的关系，包括菌株间的克隆关系。变异人我们建立了一个多位点序列分型（MLST）方案。变异体对8个管家基因片段进行了测序 ...更多信息 102个S。变异株我们在这102株分离株中鉴定出92种序列类型，表明S。变异人有着多样化的种群未发现地理特异性。然而，胶原结合蛋白基因（cnm）的分布和母婴传播的直接证据是显而易见的。我们还进行了全基因组测序的S。变异株NN 2025的基因组在整个复制区有广泛的基因组排列。(2)牙龈卟啉单胞菌（Porphyromonas gingivalis）对口腔上皮细胞的致病机制牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周致病菌，根据其菌毛fimA基因的多样性，将其菌毛分为6种基因型。结果表明，具有Ⅱ型菌毛的牙龈卟啉单胞菌比Ⅰ型菌毒力更强。用II型fimA取代I型fimA增强了细菌对上皮细胞的粘附/侵袭，而用I型fimA取代导致效率降低。这些结果表明，II型菌毛是牙龈卟啉单胞菌毒力的关键决定因素。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Inhibitory effects of tumor necrosis factor-alpha on migration of human periodontal ligament cells.

DOI：
10.1902/jop.2006.050192
发表时间：
2006-05
期刊：
Journal of periodontology
影响因子：
4.3
作者：
A. Takemura;I. Nakagawa;S. Kawai;H. Inaba;Takahiro Kato;S. Hamada;A. Amano
通讯作者：
A. Takemura;I. Nakagawa;S. Kawai;H. Inaba;Takahiro Kato;S. Hamada;A. Amano

Invasion of epithelial cells and proteolytic of cellular focal adhesion components by distinct types of Porphyromonas gingivalis

不同类型牙龈卟啉单胞菌对上皮细胞的侵袭和细胞粘着斑成分的蛋白水解