Microarray analysis to detect genes predicting organotropism of lung cancer metastasis.

肺癌易转移到多器官，如骨、肺、脑、肝、肾上腺等。我们应用微阵列分析发现影响肺癌转移嗜器官性的基因表达。使用人肺腺癌细胞系。在胸腺小鼠新鲜组织切片上培养细胞。然后，从器官切片表面收集增殖的癌细胞，再次在烧瓶中培养。然后从每个器官中获得条件细胞。微阵列分析应用于这些细胞，包括原始细胞，以检测每个器官的特定基因改变。将各器官条件细胞的基因表达与原细胞进行比较。使用非配对t检验检查器官特异性改变。我们检测到76个基因为肾上腺转移特异性改变，22个基因为肺转移，212个基因为肝转移，25个基因为肾转移，141个基因为骨转移，27个基因为肌肉转移。

Lung cancer easily metastasizes to multiple organs, such as bone, lung, brain, liver, and adrenal gland. We applied microarray analysis to find the gene expression influencing organotropism of lung cancer metastasis. Human lung adenocarcinoma cell lines were used. Cell were cultured on the fresh tissue slice of athymic mouse. After that, proliferated cancer cells were collected from the surface of organ slice and cultured in the flask again. Then the conditioned cells from each organ were obtained. Microarray analysis was applied to these cells including original cells in order to detect specific gene alteration in each organ. Gene expressions of conditioned cells obtained from each organ were compared with original cells. Organ specific alterations were checked using unpaired t-test. We detected 76 genes as adrenal metastasis specific alterations, 22 genes with lung, 212 with liver, 25 with kidney, 141 with bone, and 27 with muscle.